FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004238946> ?p ?o ?g. }

• W2004238946 endingPage "134" @default.
• W2004238946 startingPage "128" @default.
• W2004238946 abstract "A capillary electrophoresis–mass spectrometry (CE–MS) method using sheath liquid electrospray ionization interfacing was studied and optimized for the analysis of intact basic proteins. To prevent protein adsorption, capillaries with a noncovalent positively charged coating were utilized. Capillaries were coated by subsequent rinsing with solutions of Polybrene, dextran sulfate and Polybrene. The coating proved to be fully compatible with MS detection, causing no background signals and ionization suppression. The composition of the sheath liquid and BGE was optimized using the model proteins α-chymotrypsinogen A, ribonuclease A, lysozyme and cytochrome c. A sheath liquid of isopropanol–water–acetic acid (75:25:0.1, v/v/v) at 2 μL min−1 resulted in optimal signal intensities for most proteins, but caused dissociation of the heme group of cytochrome c. Optimum protein responses were obtained with a BGE of 50 mM acetic acid (pH 3.0), which allowed a baseline separation of the test protein mixture. Several minor impurities present in the mixture could be detected and provisionally identified using accurate mass and a protein modification database. The selectivity of the CE–MS system was investigated by the analysis of acetylated lysozyme. Eight highly related species, identified as non-acetylated lysozyme and lysozyme acetylated in various degrees, could be distinguished. The CE–MS system showed good reproducibility yielding interday (three weeks period) RSDs for migration time and peak area within 2% and 10%, respectively. With the CE–MS system, determination coefficients (R2) for protein concentration and peak area were higher than 0.996, whereas detection limits were between 11 and 19 nM." @default.
• W2004238946 created "2016-06-24" @default.
• W2004238946 creator A5005199763 @default.
• W2004238946 creator A5046661376 @default.
• W2004238946 creator A5070362884 @default.
• W2004238946 date "2010-09-01" @default.
• W2004238946 modified "2023-09-30" @default.
• W2004238946 title "Capillary electrophoresis–mass spectrometry of intact basic proteins using Polybrene–dextran sulfate–Polybrene-coated capillaries: System optimization and performance" @default.
• W2004238946 cites W1964636831 @default.
• W2004238946 cites W1965319823 @default.
• W2004238946 cites W1965819236 @default.
• W2004238946 cites W1970878676 @default.
• W2004238946 cites W1973976628 @default.
• W2004238946 cites W1977343606 @default.
• W2004238946 cites W1980597381 @default.
• W2004238946 cites W1980949566 @default.
• W2004238946 cites W1991670322 @default.
• W2004238946 cites W1992071827 @default.
• W2004238946 cites W1995917146 @default.
• W2004238946 cites W1999365897 @default.
• W2004238946 cites W2003436145 @default.
• W2004238946 cites W2005818688 @default.
• W2004238946 cites W2007523183 @default.
• W2004238946 cites W2012441370 @default.
• W2004238946 cites W2017559093 @default.
• W2004238946 cites W2020976582 @default.
• W2004238946 cites W2026477120 @default.
• W2004238946 cites W2043898424 @default.
• W2004238946 cites W2059134849 @default.
• W2004238946 cites W2062515117 @default.
• W2004238946 cites W2075602114 @default.
• W2004238946 cites W2078612921 @default.
• W2004238946 cites W2080976113 @default.
• W2004238946 cites W2082338683 @default.
• W2004238946 cites W2084885918 @default.
• W2004238946 cites W2087101979 @default.
• W2004238946 cites W2088655304 @default.
• W2004238946 cites W2094150300 @default.
• W2004238946 cites W2094275179 @default.
• W2004238946 cites W2094407843 @default.
• W2004238946 cites W2095261877 @default.
• W2004238946 cites W2110634737 @default.
• W2004238946 cites W2122337426 @default.
• W2004238946 cites W2170117210 @default.
• W2004238946 cites W4232289206 @default.
• W2004238946 cites W4240046864 @default.
• W2004238946 doi "https://doi.org/10.1016/j.aca.2010.08.032" @default.
• W2004238946 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20869514" @default.
• W2004238946 hasPublicationYear "2010" @default.
• W2004238946 type Work @default.
• W2004238946 sameAs 2004238946 @default.
• W2004238946 citedByCount "57" @default.
• W2004238946 countsByYear W20042389462012 @default.
• W2004238946 countsByYear W20042389462013 @default.
• W2004238946 countsByYear W20042389462014 @default.
• W2004238946 countsByYear W20042389462015 @default.
• W2004238946 countsByYear W20042389462016 @default.
• W2004238946 countsByYear W20042389462018 @default.
• W2004238946 countsByYear W20042389462019 @default.
• W2004238946 countsByYear W20042389462021 @default.
• W2004238946 countsByYear W20042389462022 @default.
• W2004238946 countsByYear W20042389462023 @default.
• W2004238946 crossrefType "journal-article" @default.
• W2004238946 hasAuthorship W2004238946A5005199763 @default.
• W2004238946 hasAuthorship W2004238946A5046661376 @default.
• W2004238946 hasAuthorship W2004238946A5070362884 @default.
• W2004238946 hasConcept C113196181 @default.
• W2004238946 hasConcept C162356407 @default.
• W2004238946 hasConcept C185592680 @default.
• W2004238946 hasConcept C2776673659 @default.
• W2004238946 hasConcept C2776966407 @default.
• W2004238946 hasConcept C28859421 @default.
• W2004238946 hasConcept C2900643 @default.
• W2004238946 hasConcept C29311851 @default.
• W2004238946 hasConcept C40684141 @default.
• W2004238946 hasConcept C43617362 @default.
• W2004238946 hasConcept C55493867 @default.
• W2004238946 hasConcept C88142848 @default.
• W2004238946 hasConceptScore W2004238946C113196181 @default.
• W2004238946 hasConceptScore W2004238946C162356407 @default.
• W2004238946 hasConceptScore W2004238946C185592680 @default.
• W2004238946 hasConceptScore W2004238946C2776673659 @default.
• W2004238946 hasConceptScore W2004238946C2776966407 @default.
• W2004238946 hasConceptScore W2004238946C28859421 @default.
• W2004238946 hasConceptScore W2004238946C2900643 @default.
• W2004238946 hasConceptScore W2004238946C29311851 @default.
• W2004238946 hasConceptScore W2004238946C40684141 @default.
• W2004238946 hasConceptScore W2004238946C43617362 @default.
• W2004238946 hasConceptScore W2004238946C55493867 @default.
• W2004238946 hasConceptScore W2004238946C88142848 @default.
• W2004238946 hasFunder F4320334893 @default.
• W2004238946 hasIssue "1" @default.
• W2004238946 hasLocation W20042389461 @default.
• W2004238946 hasLocation W20042389462 @default.
• W2004238946 hasOpenAccess W2004238946 @default.
• W2004238946 hasPrimaryLocation W20042389461 @default.
• W2004238946 hasRelatedWork W1980842581 @default.
• W2004238946 hasRelatedWork W2005037027 @default.

• next