FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004243431> ?p ?o ?g. }

• W2004243431 endingPage "557" @default.
• W2004243431 startingPage "545" @default.
• W2004243431 abstract "1. In human U373 MG astrocytoma cells agonist-induced increases in intracellular Ca2+ ([Ca2+]i) are rapidly returned towards prestimulated levels. Examination of the effect of histamine and substance P on [Ca2+]i in thapsigargin-treated cells has allowed a mechanism contributing to this effect to be characterized. 2. Histamine and substance P stimulated [3H]-inositol monophosphate ([3H]-IP1) accumulation in U373 MG cells. Concentration-response curves of [3H]-IP1 accumulation in suspensions of U373 MG cells in HEPES buffer containing 30 mM Li+ yielded best-fit EC50 values of 19.1+/-1.5 microM for histamine and 5.7+/-1.3 nM for substance P. 3. In confluent monolayers of fura-2 loaded U373 MG cells perfusion with 100 microM histamine resulted in a transient 597+/-50 nM increase in [Ca2+]i. The best-fit EC50 for histamine was 4.6+/-2.2 microM. The initial, transient, histamine response was often followed by further small transient increases in [Ca2+]i. 4. Treatment of U373 MG cells with 5 microM thapsigargin, followed by the readdition of 1.8 mM Ca2+ to the perfusion buffer, resulted in a steady-state level of [Ca2+]i 97+/-5 nM above pretreated levels (measured 400 s after readdition of Ca2+). Perfusion of histamine (100 microM, 100 s) caused a rapid decline in the thapsigargin-induced steady state level of [Ca2+]i. This effect of histamine was normally reversible upon washout. The best-fit EC50, for the histamine response was 0.8+/-0.2 microM. Substance P (10 nM, 100s) also caused a reduction in thapsigargin-induced steady-state levels of [Ca2+]i. 5. Neither 100 microM histamine nor 10 nM substance P inhibited the rate of quench of fura-2 fluorescence by Mn2+ in U373 MG cells pretreated with 5 microM thapsigargin, indicating that the depressant effect on steady-state raised [Ca2+]i was probably not due to a block of Ca2+ entry. 6. The depressant effect of histamine on [Ca2+]i was blocked by 1 microM mepyramine, and was partially reduced by pre-incubation with 1 microM staurosporine (61+/-7% reduction) and with Ro 31-8220 (24+/-10% and 50+/-6% reduction by 1 and 10 microM Ro 31-8220, respectively). Pre-incubation with H-89 did not alter the depressant effect of histamine. 7. Neither 1 microM staurosporine nor 10 microM KN-62 inhibited the binding of [3H]-mepyramine to guinea-pig cerebellar membranes, whereas it was reduced by 17+/-1% and 55+/-2% by 1 and 10 microM Ro 31-8220, respectively. However, [3H]-IP1 accumulation stimulated by histamine in U373 MG cells was not inhibited by 1 or 10 microM Ro 31-8220 and in 2 out of 3 experiments there was a significant potentiation of the response to histamine with both concentrations of Ro 31-8220. Staurosporine, 1 microM, similarly potentiated the response to 100 microM histamine in 3 out of 4 experiments. KN-62 (10 microM) did not stimulate histamine-induced [3H]-IP1 accumulation. 8. In HEPES buffer to which no Ca2+ had been added, histamine stimulated a transient 451+/-107 nM increase in [Ca2+]i. Pretreatment with 1 microM and 10 microM Ro 31-8220 did not significantly alter the initial peak response to histamine, but slowed the rate at which histamine-induced increases in [Ca2+]i were returned to prestimulated levels. Pretreatment with KN-62 had no significant effect on the response to histamine, but consistently inhibited the secondary slower phase of the decline in [Ca2+]i. H-89 did not alter the histamine response. 9. The effect of histamine in stimulating Ca2+ extrusion was not confined to U373 MG cells, since 100 microM histamine also caused a rapid decrease in steady-state levels of [Ca2+]i in thapsigargin-treated human HeLa cells. 10. The results indicate that agonists which increase [Ca2+]i via activation of phosphoinositide metabolism can also stimulate a homeostatic mechanism which acts to reduce [Ca2+]i. The balance of the evidence indicates that in U373 MG cells the latter effect most likely involves a PKC-mediated stimulation of a Ca2+-extrusion pump." @default.
• W2004243431 created "2016-06-24" @default.
• W2004243431 creator A5006367039 @default.
• W2004243431 creator A5030179478 @default.
• W2004243431 creator A5033997788 @default.
• W2004243431 creator A5064072631 @default.
• W2004243431 date "1998-02-01" @default.
• W2004243431 modified "2023-09-27" @default.
• W2004243431 title "Dual effects of histamine and substance P on intracellular calcium levels in human U373 MG astrocytoma cells: role of protein kinase C" @default.
• W2004243431 cites W145796029 @default.
• W2004243431 cites W1483631853 @default.
• W2004243431 cites W1484685406 @default.
• W2004243431 cites W1511104911 @default.
• W2004243431 cites W1591067042 @default.
• W2004243431 cites W1599213556 @default.
• W2004243431 cites W1641731911 @default.
• W2004243431 cites W1773438712 @default.
• W2004243431 cites W1840375545 @default.
• W2004243431 cites W1862817748 @default.
• W2004243431 cites W1874327812 @default.
• W2004243431 cites W1953600165 @default.
• W2004243431 cites W1964792148 @default.
• W2004243431 cites W1969728996 @default.
• W2004243431 cites W1973410410 @default.
• W2004243431 cites W1978621039 @default.
• W2004243431 cites W1980166477 @default.
• W2004243431 cites W1980436095 @default.
• W2004243431 cites W1983205633 @default.
• W2004243431 cites W1983442088 @default.
• W2004243431 cites W1992347253 @default.
• W2004243431 cites W1999508322 @default.
• W2004243431 cites W2004334599 @default.
• W2004243431 cites W2021820002 @default.
• W2004243431 cites W2034575236 @default.
• W2004243431 cites W2041468211 @default.
• W2004243431 cites W2043970026 @default.
• W2004243431 cites W2044070752 @default.
• W2004243431 cites W2045405389 @default.
• W2004243431 cites W2049361557 @default.
• W2004243431 cites W2056584404 @default.
• W2004243431 cites W2063787926 @default.
• W2004243431 cites W2067959660 @default.
• W2004243431 cites W2070753369 @default.
• W2004243431 cites W2071752363 @default.
• W2004243431 cites W2074002103 @default.
• W2004243431 cites W2075097757 @default.
• W2004243431 cites W2084154028 @default.
• W2004243431 cites W2084860232 @default.
• W2004243431 cites W2086524338 @default.
• W2004243431 cites W2093373525 @default.
• W2004243431 cites W2093703320 @default.
• W2004243431 cites W2106820006 @default.
• W2004243431 cites W2113579215 @default.
• W2004243431 cites W2114036886 @default.
• W2004243431 cites W2117227310 @default.
• W2004243431 cites W2133019280 @default.
• W2004243431 cites W2133509146 @default.
• W2004243431 cites W2141260882 @default.
• W2004243431 cites W2342095386 @default.
• W2004243431 cites W2799503982 @default.
• W2004243431 cites W38907838 @default.
• W2004243431 cites W4211131955 @default.
• W2004243431 cites W4249424055 @default.
• W2004243431 doi "https://doi.org/10.1038/sj.bjp.0701620" @default.
• W2004243431 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1565181" @default.
• W2004243431 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9504396" @default.
• W2004243431 hasPublicationYear "1998" @default.
• W2004243431 type Work @default.
• W2004243431 sameAs 2004243431 @default.
• W2004243431 citedByCount "14" @default.
• W2004243431 countsByYear W20042434312016 @default.
• W2004243431 countsByYear W20042434312022 @default.
• W2004243431 crossrefType "journal-article" @default.
• W2004243431 hasAuthorship W2004243431A5006367039 @default.
• W2004243431 hasAuthorship W2004243431A5030179478 @default.
• W2004243431 hasAuthorship W2004243431A5033997788 @default.
• W2004243431 hasAuthorship W2004243431A5064072631 @default.
• W2004243431 hasBestOaLocation W20042434312 @default.
• W2004243431 hasConcept C1122143 @default.
• W2004243431 hasConcept C126322002 @default.
• W2004243431 hasConcept C134018914 @default.
• W2004243431 hasConcept C153911025 @default.
• W2004243431 hasConcept C170493617 @default.
• W2004243431 hasConcept C181199279 @default.
• W2004243431 hasConcept C185592680 @default.
• W2004243431 hasConcept C2777146706 @default.
• W2004243431 hasConcept C2777427919 @default.
• W2004243431 hasConcept C2778470358 @default.
• W2004243431 hasConcept C519063684 @default.
• W2004243431 hasConcept C55493867 @default.
• W2004243431 hasConcept C71924100 @default.
• W2004243431 hasConcept C79879829 @default.
• W2004243431 hasConcept C86803240 @default.
• W2004243431 hasConcept C98539663 @default.
• W2004243431 hasConceptScore W2004243431C1122143 @default.
• W2004243431 hasConceptScore W2004243431C126322002 @default.
• W2004243431 hasConceptScore W2004243431C134018914 @default.
• W2004243431 hasConceptScore W2004243431C153911025 @default.

• next