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- W2004258204 abstract "Summary We investigated mitosis in amoebae of Protostelium mycophaga by video microscopy of live cells, by indirect immunofluorescence with antibodies against tubulins, and by transmission electron microscopy of ultrathin sections. Amoebae in interphase usually contain two microtubule centers (MCs) on opposite sides of the nucleus, from which microtubules (MTs) radiate into the cytoplasm. During prophase these MTs shorten to form two asters between which the mitotic spindle develops during prometaphase. Concomitantly, the nucleolus fragments, the numerous small chromosomes orient amphitelically in the spindle and congress to the spindle equator, and the asters diminish further until metaphase. The spindle is open and acentric, but with complex spindle pole bodies. Each sister-chromatid is attached to a single MT by a tiny, layered kinetochore. During anaphase and telophase, asters develop anew and enlarge to become the elaborate MT cytoskeletons of the daughter cells. Anaphase lasted 2 min on average (s.d. = 0.6 min, n = 4), during which the chromosomes moved poleward with a mean velocity of 4.0 μm/min (s.d. = 0.8 μm/min, n = 5). The intermingling of kinetochore MTs and the numerous non-kinetochore MTs allows for a sliding interaction between them, but depolymerisation-driven chromosome movement is also possible. The spindle elongated at a mean rate of 5.9 μm/min (s.d. = 2.2 μm/min, n = 5), and the mean elongation factor was 2.4 in live cells. In immunofluorescence preparations the longest spindles were 3.5 times longer than the average metaphase spindle. Spindle elongation thus requires the growth of interzonal MTs that assemble as several bundles from an ample pool of tubulin. At the end of telophase the nuclear envelope is reconstructed from membrane vesicles and flattened cisternae that appose to the masses of decondensed chromosomes and nucleolar material." @default.
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- W2004258204 title "Mitosis in Amoebae of the cellular slime mold (Mycetozoan) Protostelium mycophaga" @default.
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- W2004258204 doi "https://doi.org/10.1016/s0932-4739(11)80438-4" @default.
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