Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004262287> ?p ?o ?g. }
- W2004262287 endingPage "7610" @default.
- W2004262287 startingPage "7607" @default.
- W2004262287 abstract "Okadaic acid (OA) and dinophysistoxin-1 (DTX1) cause diarrheic shellfish poisoning. This article examines the biochemical interactions of the two toxins with novel okadaic acid binding proteins (OABPs) 2.1 and 2.3, originally isolated from the marine sponge Halichondria okadai. First, recombinant OABPs 2.1 and 2.3 were expressed in Escherichia coli BL21 (DE3) cells. Binding assays using [24-3H]OA and the recombinant OABP 2.1 or 2.3 demonstrated the dissociation constant Kd of 1.30 ± 0.56 nM and 1.54 ± 0.35 nM, respectively. Binding of [24-3H]okadaic acid to recombinant OABP2.1 was almost equally replaced with OA and DTX1. OA-induced cytotoxicity in mouse leukemia P388 cells was inhibited in the presence of the recombinant OABPs 2.1 and 2.3 with an EC50 of 92 ± 8.4 nM and 87 ± 13 nM, respectively. These results suggest that the blockage of OA-induced cytotoxicity by OABPs 2.1 and 2.3 may be involved in regulating symbiotic relationships present in the sponge H. okadai." @default.
- W2004262287 created "2016-06-24" @default.
- W2004262287 creator A5008772730 @default.
- W2004262287 creator A5027654165 @default.
- W2004262287 creator A5031965892 @default.
- W2004262287 creator A5034295734 @default.
- W2004262287 creator A5048373541 @default.
- W2004262287 creator A5054073636 @default.
- W2004262287 creator A5059663164 @default.
- W2004262287 date "2010-11-01" @default.
- W2004262287 modified "2023-10-03" @default.
- W2004262287 title "Binding of diarrheic shellfish poisoning toxins to okadaic acid binding proteins purified from the sponge Halichondria okadai" @default.
- W2004262287 cites W1528602399 @default.
- W2004262287 cites W1606849762 @default.
- W2004262287 cites W1722608155 @default.
- W2004262287 cites W1854389394 @default.
- W2004262287 cites W1960573418 @default.
- W2004262287 cites W1965297430 @default.
- W2004262287 cites W1972309401 @default.
- W2004262287 cites W1987479686 @default.
- W2004262287 cites W1992171735 @default.
- W2004262287 cites W1999672224 @default.
- W2004262287 cites W2022729618 @default.
- W2004262287 cites W2042917468 @default.
- W2004262287 cites W2048050065 @default.
- W2004262287 cites W2065188354 @default.
- W2004262287 cites W2081054598 @default.
- W2004262287 cites W2085257512 @default.
- W2004262287 cites W2089003647 @default.
- W2004262287 cites W2089969587 @default.
- W2004262287 cites W2102424000 @default.
- W2004262287 cites W2120553816 @default.
- W2004262287 cites W2144943660 @default.
- W2004262287 cites W2159332152 @default.
- W2004262287 cites W2314795980 @default.
- W2004262287 cites W2566668713 @default.
- W2004262287 cites W383713885 @default.
- W2004262287 doi "https://doi.org/10.1016/j.bmc.2010.08.043" @default.
- W2004262287 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20863709" @default.
- W2004262287 hasPublicationYear "2010" @default.
- W2004262287 type Work @default.
- W2004262287 sameAs 2004262287 @default.
- W2004262287 citedByCount "11" @default.
- W2004262287 countsByYear W20042622872013 @default.
- W2004262287 countsByYear W20042622872014 @default.
- W2004262287 countsByYear W20042622872015 @default.
- W2004262287 countsByYear W20042622872017 @default.
- W2004262287 countsByYear W20042622872019 @default.
- W2004262287 countsByYear W20042622872022 @default.
- W2004262287 countsByYear W20042622872023 @default.
- W2004262287 crossrefType "journal-article" @default.
- W2004262287 hasAuthorship W2004262287A5008772730 @default.
- W2004262287 hasAuthorship W2004262287A5027654165 @default.
- W2004262287 hasAuthorship W2004262287A5031965892 @default.
- W2004262287 hasAuthorship W2004262287A5034295734 @default.
- W2004262287 hasAuthorship W2004262287A5048373541 @default.
- W2004262287 hasAuthorship W2004262287A5054073636 @default.
- W2004262287 hasAuthorship W2004262287A5059663164 @default.
- W2004262287 hasConcept C104317684 @default.
- W2004262287 hasConcept C109316439 @default.
- W2004262287 hasConcept C153911025 @default.
- W2004262287 hasConcept C170493617 @default.
- W2004262287 hasConcept C178666793 @default.
- W2004262287 hasConcept C181199279 @default.
- W2004262287 hasConcept C185592680 @default.
- W2004262287 hasConcept C202751555 @default.
- W2004262287 hasConcept C2776953658 @default.
- W2004262287 hasConcept C2777367657 @default.
- W2004262287 hasConcept C2778849931 @default.
- W2004262287 hasConcept C2910844273 @default.
- W2004262287 hasConcept C40767141 @default.
- W2004262287 hasConcept C547475151 @default.
- W2004262287 hasConcept C55493867 @default.
- W2004262287 hasConcept C59822182 @default.
- W2004262287 hasConcept C74998103 @default.
- W2004262287 hasConcept C86803240 @default.
- W2004262287 hasConceptScore W2004262287C104317684 @default.
- W2004262287 hasConceptScore W2004262287C109316439 @default.
- W2004262287 hasConceptScore W2004262287C153911025 @default.
- W2004262287 hasConceptScore W2004262287C170493617 @default.
- W2004262287 hasConceptScore W2004262287C178666793 @default.
- W2004262287 hasConceptScore W2004262287C181199279 @default.
- W2004262287 hasConceptScore W2004262287C185592680 @default.
- W2004262287 hasConceptScore W2004262287C202751555 @default.
- W2004262287 hasConceptScore W2004262287C2776953658 @default.
- W2004262287 hasConceptScore W2004262287C2777367657 @default.
- W2004262287 hasConceptScore W2004262287C2778849931 @default.
- W2004262287 hasConceptScore W2004262287C2910844273 @default.
- W2004262287 hasConceptScore W2004262287C40767141 @default.
- W2004262287 hasConceptScore W2004262287C547475151 @default.
- W2004262287 hasConceptScore W2004262287C55493867 @default.
- W2004262287 hasConceptScore W2004262287C59822182 @default.
- W2004262287 hasConceptScore W2004262287C74998103 @default.
- W2004262287 hasConceptScore W2004262287C86803240 @default.
- W2004262287 hasIssue "21" @default.
- W2004262287 hasLocation W20042622871 @default.
- W2004262287 hasLocation W20042622872 @default.
- W2004262287 hasOpenAccess W2004262287 @default.