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• W2004302574 abstract "S-100 was shown to regulate the in vitro assembly of brain microtubule proteins (MTPs) in a Ca2+-mediated way by acting on both the nucleation and the elongation of microtubules (MTs). Here data will be shown suggesting that S-100 binds to tubulin. The binding is time-, temperature-, Ca2+-, and pH-dependent, and saturable with respect to S-100. At pH 6.75, the saturation curve is biphasic, displaying a high affinity component (dissociation constant, Kd1,∼0.1 μM) and a low affinity component (Kd2 ∼ 3.8 μM). At pH 6.75, as the free Ca2+ concentration raises from 0 to 100 μM, the overall binding capacity increases from 0.065 to 0.66 mol S-100/mol tubulin dimer. This finding, together with the observation that the S-100 effect on MTP assembly is Ca2+-dependent at that pH, suggests that the S-100-induced inhibition of MTP assembly depends on S-100 binding to the low affinity sites on the tubulin molecule. The S-100 binding to tubulin is pH-dependent: as the pH raises from 6.75 to 8.3, both binding components are affected, the major changes consisting of an increase in the binding capacity and a decrease in the overall affinity. Moreover, as the pH raises, Ca2+ is no longer required for S-100 to bind to tubulin. S-100 also interacts with a component of whole MTPs (probably tubulin, on the basis of the above results). No S-100 binding to microtubule-associated proteins (MAPs) could be evidenced by the techniques employed in this study. On the contrary, some competition between S-100 and MAPs for binding sites or tubulin seems to occur." @default.
• W2004302574 created "2016-06-24" @default.
• W2004302574 creator A5065739247 @default.
• W2004302574 date "1987-08-01" @default.
• W2004302574 modified "2023-10-01" @default.
• W2004302574 title "Quantitative analysis of the interaction between S-100 proteins and brain tubulin" @default.
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• W2004302574 doi "https://doi.org/10.1016/0143-4160(87)90004-2" @default.
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