Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004304118> ?p ?o ?g. }
- W2004304118 endingPage "445" @default.
- W2004304118 startingPage "433" @default.
- W2004304118 abstract "Ndel1 has been implicated in a variety of dynein-related processes, but its specific function is unclear. Here we describe an experimental approach to evaluate a role of Ndel1 in dynein-dependent microtubule self-organization using Ran-mediated asters in meiotic Xenopus egg extracts. We demonstrate that extracts depleted of Ndel1 are unable to form asters and that this defect can be rescued by the addition of recombinant N-terminal coiled-coil domain of Ndel1. Ndel1-dependent microtubule self-organization requires an interaction between Ndel1 and dynein, which is mediated by the dimerization fragment of the coiled-coil. Full rescue by the coiled-coil domain requires LIS1 binding, and increasing LIS1 concentration partly rescues aster formation, suggesting that Ndel1 is a recruitment factor for LIS1. The interactions between Ndel1 and its binding partners are positively regulated by phosphorylation of the unstructured C terminus. Together, our results provide important insights into how Ndel1 acts as a regulated scaffold to temporally and spatially regulate dynein." @default.
- W2004304118 created "2016-06-24" @default.
- W2004304118 creator A5004401237 @default.
- W2004304118 creator A5030692365 @default.
- W2004304118 creator A5046085173 @default.
- W2004304118 creator A5055855625 @default.
- W2004304118 creator A5072633316 @default.
- W2004304118 creator A5080893332 @default.
- W2004304118 date "2011-01-31" @default.
- W2004304118 modified "2023-10-12" @default.
- W2004304118 title "The N-terminal coiled-coil of Ndel1 is a regulated scaffold that recruits LIS1 to dynein" @default.
- W2004304118 cites W1481912803 @default.
- W2004304118 cites W1512975085 @default.
- W2004304118 cites W1531700940 @default.
- W2004304118 cites W1565504254 @default.
- W2004304118 cites W1966723276 @default.
- W2004304118 cites W1972377722 @default.
- W2004304118 cites W1985169370 @default.
- W2004304118 cites W1988573682 @default.
- W2004304118 cites W1998728980 @default.
- W2004304118 cites W2002694151 @default.
- W2004304118 cites W2007764809 @default.
- W2004304118 cites W2013655553 @default.
- W2004304118 cites W2014982026 @default.
- W2004304118 cites W2026353278 @default.
- W2004304118 cites W2033062977 @default.
- W2004304118 cites W2033589415 @default.
- W2004304118 cites W2036623899 @default.
- W2004304118 cites W2037835429 @default.
- W2004304118 cites W2047308178 @default.
- W2004304118 cites W2064062498 @default.
- W2004304118 cites W2067890396 @default.
- W2004304118 cites W2072278366 @default.
- W2004304118 cites W2075366096 @default.
- W2004304118 cites W2085102956 @default.
- W2004304118 cites W2095790288 @default.
- W2004304118 cites W2104988846 @default.
- W2004304118 cites W2105851524 @default.
- W2004304118 cites W2106492464 @default.
- W2004304118 cites W2108590463 @default.
- W2004304118 cites W2110488004 @default.
- W2004304118 cites W2117492959 @default.
- W2004304118 cites W2120375547 @default.
- W2004304118 cites W2128647989 @default.
- W2004304118 cites W2130217713 @default.
- W2004304118 cites W2133326351 @default.
- W2004304118 cites W2145615636 @default.
- W2004304118 cites W2146982832 @default.
- W2004304118 cites W2164372658 @default.
- W2004304118 cites W2164582507 @default.
- W2004304118 cites W4246573955 @default.
- W2004304118 cites W935855719 @default.
- W2004304118 doi "https://doi.org/10.1083/jcb.201011142" @default.
- W2004304118 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3101096" @default.
- W2004304118 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21282465" @default.
- W2004304118 hasPublicationYear "2011" @default.
- W2004304118 type Work @default.
- W2004304118 sameAs 2004304118 @default.
- W2004304118 citedByCount "75" @default.
- W2004304118 countsByYear W20043041182012 @default.
- W2004304118 countsByYear W20043041182013 @default.
- W2004304118 countsByYear W20043041182014 @default.
- W2004304118 countsByYear W20043041182015 @default.
- W2004304118 countsByYear W20043041182016 @default.
- W2004304118 countsByYear W20043041182017 @default.
- W2004304118 countsByYear W20043041182018 @default.
- W2004304118 countsByYear W20043041182019 @default.
- W2004304118 countsByYear W20043041182020 @default.
- W2004304118 countsByYear W20043041182021 @default.
- W2004304118 countsByYear W20043041182022 @default.
- W2004304118 countsByYear W20043041182023 @default.
- W2004304118 crossrefType "journal-article" @default.
- W2004304118 hasAuthorship W2004304118A5004401237 @default.
- W2004304118 hasAuthorship W2004304118A5030692365 @default.
- W2004304118 hasAuthorship W2004304118A5046085173 @default.
- W2004304118 hasAuthorship W2004304118A5055855625 @default.
- W2004304118 hasAuthorship W2004304118A5072633316 @default.
- W2004304118 hasAuthorship W2004304118A5080893332 @default.
- W2004304118 hasBestOaLocation W20043041181 @default.
- W2004304118 hasConcept C104317684 @default.
- W2004304118 hasConcept C106987784 @default.
- W2004304118 hasConcept C20418707 @default.
- W2004304118 hasConcept C2779535977 @default.
- W2004304118 hasConcept C53645450 @default.
- W2004304118 hasConcept C54355233 @default.
- W2004304118 hasConcept C55988834 @default.
- W2004304118 hasConcept C62478195 @default.
- W2004304118 hasConcept C86803240 @default.
- W2004304118 hasConcept C95444343 @default.
- W2004304118 hasConceptScore W2004304118C104317684 @default.
- W2004304118 hasConceptScore W2004304118C106987784 @default.
- W2004304118 hasConceptScore W2004304118C20418707 @default.
- W2004304118 hasConceptScore W2004304118C2779535977 @default.
- W2004304118 hasConceptScore W2004304118C53645450 @default.
- W2004304118 hasConceptScore W2004304118C54355233 @default.
- W2004304118 hasConceptScore W2004304118C55988834 @default.
- W2004304118 hasConceptScore W2004304118C62478195 @default.
- W2004304118 hasConceptScore W2004304118C86803240 @default.