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- W2004309887 abstract "A new method for determining the degree of N-acetylation of chitosan is proposed, which is based on ultraviolet (u.v.) absorbance at 220 nm of acetamide groups. Using the molar absorption coefficient of the acetamide group of N-acetyl-d-glucosamine and N,N′-diacetyl-d-chitobiose, the degree of N-acetylation of chitosan could be calculated. This principle was applied on high pressure liquid chromatography with an aqueous gel permeation support and a.u.v. detector. It was found that u.v. absorption peak areas in chromatograms were proportional to the concentration of acetamide groups and by this relationship the degree of N-acetylation could be estimated. This method is very useful for the characterization of chitosan because not only the degree of N-acetylation but also molecular weight can be measured at a time. This work indicates that the method is quite useful for tracing the time course of reactions between chitosan and reagents (acetic anhydride and others) because low molecular weight compounds are swept out from the main peak of chitosan in the chromatogram and the separation and purification of a reaction product can be omitted." @default.
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- W2004309887 date "1986-06-01" @default.
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- W2004309887 title "Studies on chitosan: 1. Determination of the degree of N-acetylation of chitosan by ultraviolet spectrophotometry and gel permeation chromatography" @default.
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- W2004309887 doi "https://doi.org/10.1016/0141-8130(86)90022-x" @default.
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