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- W2004321965 abstract "Rabbit T lymphocytes with cytotoxic activity for mouse target cells can be maintained in culture for several weeks by periodically restimulating with fresh mouse cells or by culturing with a source of TCGF. The former procedure greatly enriches the cultured cells for cytotoxic activity but has the disadvantage of introducing cellular debris. Cell growth and cytotoxic activity waned after 4-6 weeks using either procedure. However, T lymphocyte lines (TLL) could be isolated from limiting dilution cultures containing mitomycin C-treated mouse 'feeder' cells and a source of TCGF. These cells grew out with a frequency of one in 50-150 and approximately 15% of these growing cells had cytotoxic activity. Some cytotoxic cells lost their lytic activity after 4-5 months but others have persisted for over 7 months. Some TLL have now been growing for over 20 months." @default.
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- W2004321965 date "1983-01-01" @default.
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- W2004321965 title "In vitro studies of the rabbit immune system. IX. Extended culture of rabbit cytotoxic cells and the generation of TCGF-dependent T lymphocyte lines" @default.
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- W2004321965 doi "https://doi.org/10.1016/s0022-1759(83)80026-x" @default.
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