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• W2004353555 endingPage "80" @default.
• W2004353555 startingPage "71" @default.
• W2004353555 abstract "β2-ARs (β2-adrenoceptors) become desensitized rapidly upon recruitment of cytosolic β-arrestin. PDE4D5 (family 4 cAMP-specific phosphodiesterase, subfamily D, isoform 5) can be recruited in complex with β-arrestin, whereupon it regulates PKA (cAMP-dependent protein kinase) phosphorylation of the β2-AR. In the present study, we have used novel technology, employing a library of overlapping peptides (25-mers) immobilized on cellulose membranes that scan the entire sequence of β-arrestin 2, to define the interaction sites on β-arrestin 2 for binding of PDE4D5 and the cognate long isoform, PDE4D3. We have identified a binding site in the β-arrestin 2 N-domain for the common PDE4D catalytic unit and two regions in the β-arrestin 2 C-domain that confer specificity for PDE4D5 binding. Alanine-scanning peptide array analysis of the N-domain binding region identified severely reduced interaction with PDE4D5 upon R26A substitution, and reduced interaction upon either K18A or T20A substitution. Similar analysis of the β-arrestin 2 C-domain identified Arg286 and Asp291, together with the Leu215–His220 region, as being important for binding PDE4D5, but not PDE4D3. Transfection with wild-type β-arrestin 2 profoundly decreased isoprenaline-stimulated PKA phosphorylation of the β2-AR in MEFs (mouse embryo fibroblasts) lacking both β-arrestin 1 and β-arrestin 2. This effect was negated using either the R26A or the R286A mutant form of β-arrestin 2 or a mutant with substitution of an alanine cassette for Leu215–His220, which showed little or no PDE4D5 binding, but was still recruited to the β2-AR upon isoprenaline challenge. These data show that the interaction of PDE4D5 with both the N- and C-domains of β-arrestin 2 are essential for β2-AR regulation." @default.
• W2004353555 created "2016-06-24" @default.
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• W2004353555 date "2007-04-26" @default.
• W2004353555 modified "2023-09-23" @default.
• W2004353555 title "Mapping binding sites for the PDE4D5 cAMP-specific phosphodiesterase to the N- and C-domains of β-arrestin using spot-immobilized peptide arrays" @default.
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• W2004353555 doi "https://doi.org/10.1042/bj20070005" @default.
• W2004353555 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1868836" @default.
• W2004353555 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17288540" @default.
• W2004353555 hasPublicationYear "2007" @default.
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