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- W2004357150 abstract "Applying various restriction enzymes on a specially designed 1.5 kb DNA fragment revealed that the inhibitory effects of PUVA treatment on restriction endonuclease activities are caused by recognition inhibition. In this study, Restriction enzymes which have a 5'-TpA sequence at the cleaving site (Kpn I, Xba I, Pme I, and Dra I), and non-cleaving site (Pac I) in recognition sites, or have two 5'-TpA sequences at the recognition site and a non-specific sequence between recognition and cleaving site (BciV I) were inhibited by PUVA treatment. Most of the other restriction enzymes used in this study which do not have a 5'-TpA sequence at their restriction site were not inhibited by PUVA treatment, although a 5'-TpA sequence is located adjacent (Sma I) or very close (BamH I, Sac I and Pst I) to the recognition and cleaving site for them." @default.
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- W2004357150 date "2003-09-01" @default.
- W2004357150 modified "2023-09-24" @default.
- W2004357150 title "Inhibition of restriction enzyme's DNA sequence recognition by PUVA treatment" @default.
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- W2004357150 doi "https://doi.org/10.1093/nass/3.1.297" @default.
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