FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004364394> ?p ?o ?g. }

• W2004364394 endingPage "261" @default.
• W2004364394 startingPage "255" @default.
• W2004364394 abstract "B cell epitopes of the major house dust mite allergen Der f 2 from Dermatophagoides farinae were analysed using deletion mutants of Der f 2 expressed as fusion proteins in Escherichia coli. The reactivities of these partial Der f 2 molecules to human anti-mite IgE antibodies in atopic patients and to murine anti-Der f 2 monoclonal antibodies (mAbs) were examined by immunoblotting. A C-terminal deletion mutant of Der f 2, 1–123, had almost the same reactivity to human IgE as the whole Der f 2 (1–129) and an N-terminal deletion mutant of Der f 2 (25–129) still had weak reactivity. On the other hand, in two deleted Der f 2 molecules, 1–120 and 30–129, reactivity was lost in spite of long overlapping sequences. These results suggest that the human IgE antibodies to Der f 2 in atopic patient sera recognize the conformational structures dependent on the tertiary structure of Der f 2, including disulfide bond formations, rather than the contiguous sequences of amino acids. The sequences 1–24, 25–29 and 121–123 were revealed as the minimum N-and C- terminal amino acid sequences required for IgE binding. Contrastingly, all three murine mAbs bound to the smaller deletion mutants, 1–90 and 67–129, suggesting that the cores of the epitopes for these mAbs exist in the 24 amino acid sequence of Der f 2, 67–90 overlapping the sequential human IgE epitope on Der p 2, the equivalent allergen from Dermatophagoides pteronyssinus. These findings are important for the understanding of the antigenic structure of Der f 2 and for the manipulation of the allergen for immunotherapy." @default.
• W2004364394 created "2016-06-24" @default.
• W2004364394 creator A5015488402 @default.
• W2004364394 creator A5036869700 @default.
• W2004364394 creator A5053000890 @default.
• W2004364394 creator A5065515771 @default.
• W2004364394 creator A5080139718 @default.
• W2004364394 date "1997-02-01" @default.
• W2004364394 modified "2023-10-18" @default.
• W2004364394 title "Determination of the N- and C-terminal sequences required to bind human IgE of the major house dust mite allergen Der f 2 and epitope mapping for monoclonal antibodies" @default.
• W2004364394 cites W1487177241 @default.
• W2004364394 cites W1512922956 @default.
• W2004364394 cites W1528328673 @default.
• W2004364394 cites W1626813947 @default.
• W2004364394 cites W1679527748 @default.
• W2004364394 cites W1888308862 @default.
• W2004364394 cites W1910526658 @default.
• W2004364394 cites W1925788296 @default.
• W2004364394 cites W1973368746 @default.
• W2004364394 cites W2004306315 @default.
• W2004364394 cites W2005579644 @default.
• W2004364394 cites W2009048532 @default.
• W2004364394 cites W2021659405 @default.
• W2004364394 cites W2024249282 @default.
• W2004364394 cites W2028932256 @default.
• W2004364394 cites W2029693770 @default.
• W2004364394 cites W2032462421 @default.
• W2004364394 cites W2038226520 @default.
• W2004364394 cites W2039109411 @default.
• W2004364394 cites W2047695535 @default.
• W2004364394 cites W2054079378 @default.
• W2004364394 cites W2060349968 @default.
• W2004364394 cites W2074639833 @default.
• W2004364394 cites W2078072837 @default.
• W2004364394 cites W2079218165 @default.
• W2004364394 cites W2081676212 @default.
• W2004364394 cites W2082543043 @default.
• W2004364394 cites W2087887110 @default.
• W2004364394 cites W2088680288 @default.
• W2004364394 cites W2090687924 @default.
• W2004364394 cites W2092289268 @default.
• W2004364394 cites W2100837269 @default.
• W2004364394 cites W2101108802 @default.
• W2004364394 cites W2134889315 @default.
• W2004364394 cites W2140152586 @default.
• W2004364394 cites W2155750360 @default.
• W2004364394 cites W2164025040 @default.
• W2004364394 cites W2169581200 @default.
• W2004364394 cites W2339514173 @default.
• W2004364394 cites W41460957 @default.
• W2004364394 cites W75178188 @default.
• W2004364394 doi "https://doi.org/10.1016/s0161-5890(97)00020-5" @default.
• W2004364394 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9224967" @default.
• W2004364394 hasPublicationYear "1997" @default.
• W2004364394 type Work @default.
• W2004364394 sameAs 2004364394 @default.
• W2004364394 citedByCount "43" @default.
• W2004364394 countsByYear W20043643942012 @default.
• W2004364394 countsByYear W20043643942013 @default.
• W2004364394 countsByYear W20043643942015 @default.
• W2004364394 countsByYear W20043643942016 @default.
• W2004364394 countsByYear W20043643942017 @default.
• W2004364394 countsByYear W20043643942019 @default.
• W2004364394 countsByYear W20043643942020 @default.
• W2004364394 countsByYear W20043643942022 @default.
• W2004364394 crossrefType "journal-article" @default.
• W2004364394 hasAuthorship W2004364394A5015488402 @default.
• W2004364394 hasAuthorship W2004364394A5036869700 @default.
• W2004364394 hasAuthorship W2004364394A5053000890 @default.
• W2004364394 hasAuthorship W2004364394A5065515771 @default.
• W2004364394 hasAuthorship W2004364394A5080139718 @default.
• W2004364394 hasConcept C104317684 @default.
• W2004364394 hasConcept C141105273 @default.
• W2004364394 hasConcept C143065580 @default.
• W2004364394 hasConcept C153911025 @default.
• W2004364394 hasConcept C159654299 @default.
• W2004364394 hasConcept C167625842 @default.
• W2004364394 hasConcept C185592680 @default.
• W2004364394 hasConcept C195616568 @default.
• W2004364394 hasConcept C203014093 @default.
• W2004364394 hasConcept C207480886 @default.
• W2004364394 hasConcept C2778166374 @default.
• W2004364394 hasConcept C2779673145 @default.
• W2004364394 hasConcept C2780510475 @default.
• W2004364394 hasConcept C2781241564 @default.
• W2004364394 hasConcept C515207424 @default.
• W2004364394 hasConcept C542903549 @default.
• W2004364394 hasConcept C55493867 @default.
• W2004364394 hasConcept C86803240 @default.
• W2004364394 hasConceptScore W2004364394C104317684 @default.
• W2004364394 hasConceptScore W2004364394C141105273 @default.
• W2004364394 hasConceptScore W2004364394C143065580 @default.
• W2004364394 hasConceptScore W2004364394C153911025 @default.
• W2004364394 hasConceptScore W2004364394C159654299 @default.
• W2004364394 hasConceptScore W2004364394C167625842 @default.
• W2004364394 hasConceptScore W2004364394C185592680 @default.
• W2004364394 hasConceptScore W2004364394C195616568 @default.
• W2004364394 hasConceptScore W2004364394C203014093 @default.

• next