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- W2004367452 abstract "Taxol and vincristine belong to a group of anticancer drugs that target microtubules, subsequently arresting cells at the mitotic phase of the cell cycle and inducing programmed cell death. The BCL2 ( bcl‐2 ) family of genes is of known implication in apoptosis induced by various stimuli, among which BCL2L12, a new member of the family, cloned by our group. For further insights into the mechanisms and molecular targets implicated and modified as a result of apoptosis induced by these two mitosis‐arresting drugs, we studied the possible alterations, at the mRNA level, of various apoptosis‐related genes ( BCL2, BAX, BCL2L12, CASPASE‐3, FAS ) after leukemia cell (HL‐60) treatment with these drugs. The kinetics of cell toxicity were evaluated by the MTT [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] method, trypan blue staining, and cell proliferation efficiency; apoptosis induction was assayed by endonucleosomal cleavage of DNA (DNA laddering); and the expression levels of the genes were analysed by RT‐PCR, using gene‐specific primers. The percentage of nonviable cells was upregulated with increasing cell exposure time and drug concentrations to both taxol and vincristine. Distinct modulations of apoptosis‐related genes at the mRNA level were also observed, mainly concerning BCL2 and BCL2L12 along apoptosis induction. Our results indicate and support the hypothesis that the apoptosis‐related genes BCL2 and BCL2L12 respond similarly to treatment of the human, acute, myelocytic leukemia HL60 cells with the anticancer drugs vincristine and taxol though in a drug‐specific and time‐dependent manner." @default.
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- W2004367452 date "2009-08-01" @default.
- W2004367452 modified "2023-09-24" @default.
- W2004367452 title "Molecular Response of HL-60 Cells to Mitotic Inhibitors Vincristine and Taxol Visualized with Apoptosis-Related Gene Expressions, Including the New Member BCL2L12" @default.
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- W2004367452 doi "https://doi.org/10.1111/j.1749-6632.2009.04912.x" @default.
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