Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004368167> ?p ?o ?g. }
- W2004368167 endingPage "62" @default.
- W2004368167 startingPage "54" @default.
- W2004368167 abstract "Abstract Pluripotent cells can be isolated from the human blastocyst and maintained in culture as self-renewing, undifferentiated, human ESCs (hESCs). These cells are a valuable model of human development in vitro and are the focus of substantial research aimed at generating differentiated populations for cellular therapies. The extracellular markers that have been used to characterize hESCs are primarily carbohydrate epitopes on proteoglycans or sphingolipids, such as stage-specific embryonic antigen (SSEA)-3 and -4. The expression of SSEA-3 and -4 is tightly regulated during preimplantation development and on hESCs. Although this might imply a molecular function in undifferentiated cells, it has not yet been tested experimentally. We used inhibitors of sphingolipid and glycosphingolipid (GSL) biosynthesis to block the generation of SSEA-3 and -4 in hESCs. Depletion of these antigens and their precursors was confirmed using immunostaining, flow cytometry, and tandem mass spectroscopy. Transcriptional analysis, immunostaining, and differentiation in vitro and in teratomas indicated that other properties of pluripotency were not noticeably affected by GSL depletion. These experiments demonstrated that the GSLs recognized as SSEA-3 and -4 do not play critical functional roles in maintaining the pluripotency of hESCs, but instead suggested roles for this class of molecules during cellular differentiation." @default.
- W2004368167 created "2016-06-24" @default.
- W2004368167 creator A5002672605 @default.
- W2004368167 creator A5003227513 @default.
- W2004368167 creator A5010898686 @default.
- W2004368167 creator A5012354760 @default.
- W2004368167 creator A5049246835 @default.
- W2004368167 creator A5069003187 @default.
- W2004368167 creator A5087689048 @default.
- W2004368167 creator A5091696771 @default.
- W2004368167 date "2006-09-28" @default.
- W2004368167 modified "2023-10-02" @default.
- W2004368167 title "The Cell Surface Glycosphingolipids SSEA-3 and SSEA-4 Are Not Essential for Human ESC Pluripotency" @default.
- W2004368167 cites W1491772324 @default.
- W2004368167 cites W1518310590 @default.
- W2004368167 cites W1532405552 @default.
- W2004368167 cites W1599568627 @default.
- W2004368167 cites W1771512800 @default.
- W2004368167 cites W1975065220 @default.
- W2004368167 cites W1975141582 @default.
- W2004368167 cites W1980243879 @default.
- W2004368167 cites W1980588089 @default.
- W2004368167 cites W1981088384 @default.
- W2004368167 cites W1982501879 @default.
- W2004368167 cites W1989780518 @default.
- W2004368167 cites W2001582279 @default.
- W2004368167 cites W2001743581 @default.
- W2004368167 cites W2005296007 @default.
- W2004368167 cites W2010557052 @default.
- W2004368167 cites W2013975443 @default.
- W2004368167 cites W2022399725 @default.
- W2004368167 cites W2030983339 @default.
- W2004368167 cites W2032315776 @default.
- W2004368167 cites W2036087370 @default.
- W2004368167 cites W2042699407 @default.
- W2004368167 cites W2056707698 @default.
- W2004368167 cites W2061397088 @default.
- W2004368167 cites W2065268746 @default.
- W2004368167 cites W2076091755 @default.
- W2004368167 cites W2076534093 @default.
- W2004368167 cites W2080227154 @default.
- W2004368167 cites W2086943348 @default.
- W2004368167 cites W2088088839 @default.
- W2004368167 cites W2091790925 @default.
- W2004368167 cites W2091996572 @default.
- W2004368167 cites W2094557730 @default.
- W2004368167 cites W2097543443 @default.
- W2004368167 cites W2101650913 @default.
- W2004368167 cites W2106244437 @default.
- W2004368167 cites W2107575419 @default.
- W2004368167 cites W2113415712 @default.
- W2004368167 cites W2127031535 @default.
- W2004368167 cites W2133501984 @default.
- W2004368167 cites W2136006096 @default.
- W2004368167 cites W2138442302 @default.
- W2004368167 cites W2148048540 @default.
- W2004368167 cites W2149523673 @default.
- W2004368167 cites W2155062057 @default.
- W2004368167 cites W2157885594 @default.
- W2004368167 cites W2158343596 @default.
- W2004368167 cites W2410060723 @default.
- W2004368167 cites W4211181668 @default.
- W2004368167 doi "https://doi.org/10.1634/stemcells.2006-0232" @default.
- W2004368167 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17008424" @default.
- W2004368167 hasPublicationYear "2006" @default.
- W2004368167 type Work @default.
- W2004368167 sameAs 2004368167 @default.
- W2004368167 citedByCount "100" @default.
- W2004368167 countsByYear W20043681672012 @default.
- W2004368167 countsByYear W20043681672013 @default.
- W2004368167 countsByYear W20043681672014 @default.
- W2004368167 countsByYear W20043681672015 @default.
- W2004368167 countsByYear W20043681672016 @default.
- W2004368167 countsByYear W20043681672017 @default.
- W2004368167 countsByYear W20043681672018 @default.
- W2004368167 countsByYear W20043681672019 @default.
- W2004368167 countsByYear W20043681672020 @default.
- W2004368167 countsByYear W20043681672021 @default.
- W2004368167 countsByYear W20043681672022 @default.
- W2004368167 countsByYear W20043681672023 @default.
- W2004368167 crossrefType "journal-article" @default.
- W2004368167 hasAuthorship W2004368167A5002672605 @default.
- W2004368167 hasAuthorship W2004368167A5003227513 @default.
- W2004368167 hasAuthorship W2004368167A5010898686 @default.
- W2004368167 hasAuthorship W2004368167A5012354760 @default.
- W2004368167 hasAuthorship W2004368167A5049246835 @default.
- W2004368167 hasAuthorship W2004368167A5069003187 @default.
- W2004368167 hasAuthorship W2004368167A5087689048 @default.
- W2004368167 hasAuthorship W2004368167A5091696771 @default.
- W2004368167 hasBestOaLocation W20043681671 @default.
- W2004368167 hasConcept C104317684 @default.
- W2004368167 hasConcept C107459253 @default.
- W2004368167 hasConcept C143948831 @default.
- W2004368167 hasConcept C145103041 @default.
- W2004368167 hasConcept C147483822 @default.
- W2004368167 hasConcept C148738053 @default.
- W2004368167 hasConcept C153911025 @default.
- W2004368167 hasConcept C195616568 @default.