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• W2004372657 abstract "During passage through the epididymis, sperm interact with secreted epididymal proteins that promote maturation, including the acquisition of motility and fertilization competence. Viewed previously as distinct from sperm maturation, host defence capabilities are now recognized functions of the human epididymis 2 (HE2) family of sperm-binding proteins. We analysed the potent dose and time-dependent bactericidal activity of recombinant HE2alpha, HE2beta1 and HE2beta2 and found that the full-length proteins (10 microg/ml or approximately 1 microM) caused more than a 50% decrease in Escherichia coli colony forming units within 15 min. By contrast, human beta-defensin-1, at a similar concentration, required more than 90 min to exhibit similar antibacterial activity. The epididymis-specific lipocalin, LCN6, failed to kill bacteria. Higher concentrations (25-100 microg/ml) of HE2 proteins and a longer duration of treatment resulted in near total inhibition of bacterial growth. The C-terminal peptides of HE2alpha, HEbeta1 and HEbeta2 proteins exhibited antibacterial activity similar to their full-length counterparts, indicating that the antibacterial activity of HE2 proteins resides in these C-terminal regions. Antibacterial activities of HE2 proteins and peptides were slightly inhibited by NaCl concentrations of up to 150 mM, while human beta-defensin-1 activity was nearly eliminated. Reduction and alkylation of disulphide bonds in HE2 proteins and their C-terminal peptides abolished their antibacterial activity. Consistent with the ability to kill bacteria, full-length HE2 proteins and C-terminal peptides caused rapid dose-dependent permeabilization of outer and cytoplasmic E. coli membranes. A much longer exposure time was required for human beta-defensin-1-mediated permeabilization of membranes, suggesting a possible difference in mode of action compared with the HE2 antibacterial peptides." @default.
• W2004372657 created "2016-06-24" @default.
• W2004372657 creator A5000414493 @default.
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• W2004372657 date "2003-06-01" @default.
• W2004372657 modified "2023-09-26" @default.
• W2004372657 title "Antibacterial properties of the sperm-binding proteins and peptides of human epididymis 2 (HE2) family; salt sensitivity, structural dependence and their interaction with outer and cytoplasmic membranes of Escherichia coli" @default.
• W2004372657 cites W1514460208 @default.
• W2004372657 cites W1662185404 @default.
• W2004372657 cites W1671715542 @default.
• W2004372657 cites W1714792208 @default.
• W2004372657 cites W190321304 @default.
• W2004372657 cites W1964611156 @default.
• W2004372657 cites W1971891360 @default.
• W2004372657 cites W1989754171 @default.
• W2004372657 cites W1990519548 @default.
• W2004372657 cites W1995412010 @default.
• W2004372657 cites W1997683664 @default.
• W2004372657 cites W2003308630 @default.
• W2004372657 cites W2004373761 @default.
• W2004372657 cites W2005284755 @default.
• W2004372657 cites W2009459441 @default.
• W2004372657 cites W2014035436 @default.
• W2004372657 cites W2014669328 @default.
• W2004372657 cites W2017268899 @default.
• W2004372657 cites W2017709657 @default.
• W2004372657 cites W2022021179 @default.
• W2004372657 cites W2022028604 @default.
• W2004372657 cites W2023604719 @default.
• W2004372657 cites W2027359153 @default.
• W2004372657 cites W2028501275 @default.
• W2004372657 cites W2032368629 @default.
• W2004372657 cites W2054602599 @default.
• W2004372657 cites W2060300255 @default.
• W2004372657 cites W2065766479 @default.
• W2004372657 cites W2067812655 @default.
• W2004372657 cites W2075104520 @default.
• W2004372657 cites W2075322040 @default.
• W2004372657 cites W2084263146 @default.
• W2004372657 cites W2085929853 @default.
• W2004372657 cites W2091604858 @default.
• W2004372657 cites W2096283909 @default.
• W2004372657 cites W2097098774 @default.
• W2004372657 cites W2098083781 @default.
• W2004372657 cites W2101110341 @default.
• W2004372657 cites W2103302348 @default.
• W2004372657 cites W2107803079 @default.
• W2004372657 cites W2108246317 @default.
• W2004372657 cites W2108983151 @default.
• W2004372657 cites W2111584103 @default.
• W2004372657 cites W2111667046 @default.
• W2004372657 cites W2114812888 @default.
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• W2004372657 cites W2130479394 @default.
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• W2004372657 cites W2130885148 @default.
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• W2004372657 cites W2137832760 @default.
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• W2004372657 cites W2143966366 @default.
• W2004372657 cites W2148067829 @default.
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• W2004372657 doi "https://doi.org/10.1042/bj20030225" @default.
• W2004372657 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1223422" @default.
• W2004372657 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12628001" @default.
• W2004372657 hasPublicationYear "2003" @default.
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