FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004402071> ?p ?o ?g. }

• W2004402071 endingPage "698" @default.
• W2004402071 startingPage "688" @default.
• W2004402071 abstract "Background/Aims: In a previous study, we have demonstrated that primary human hepatocytes in culture are susceptible for Plasmodium falciparum sporozoite invasion and for development of parasites into exoerythrocytic forms. In a separate study we demonstrated the involvement of two human liver plasma membrane proteins (55 kD and 20 kD) in the invasion of P. falciparum sporozoites in vitro. In this study we have unravelled the nature of the 55 kD protein. Methods: For the identification of this protein, a 53–58 kD membrane protein fraction from human liver was isolated, radioactively labelled, incubated with sporozoites and cross-linked. After reduction of the cross-linker, the released proteins were mixed with unlabelled 53–58 kD protein fraction and separated on two-dimensional SDS-PAGE. Autoradiography showed a single spot corresponding to a protein of 55 kD and pI of 5.7–5.8. Results: Amino acid sequencing revealed the 55 kD protein as carboxylesterase. The biological activity of purified human liver carboxylesterase and of an anti-serum against carboxylesterase on sporozoite invasion in vitro was evaluated. Human carboxylesterase as well as a rabbit antiserum against carboxylesterase inhibited the invasion of P. falciparum sporozoites into primary human hepatocytes in culture. A number of carboxylesterase cDNA clones were isolated from a human liver cDNA library. Sequence analysis revealed two different iso-types. Immunoaffinity purified recombinant human carboxylesterase was shown also to inhibit the invasion of sporozoites into primary human hepatocytes. Immunocytochemical analysis of the localisation of carboxylesterase in primary cultures of human hepatocytes using specific antibodies, showed its presence inside the hepatocytes and on the membrane. Conclusions: Carboxylesterase plays a role in the invasion process of P. falciparum sporozoites into human hepatocytes in vitro. The implications of these findings are further discussed." @default.
• W2004402071 created "2016-06-24" @default.
• W2004402071 creator A5000220586 @default.
• W2004402071 creator A5011594748 @default.
• W2004402071 creator A5021852823 @default.
• W2004402071 creator A5041185165 @default.
• W2004402071 creator A5080124451 @default.
• W2004402071 date "1997-10-01" @default.
• W2004402071 modified "2023-09-23" @default.
• W2004402071 title "Identification of human liver carboxylesterase as one of the proteins involved in Plasmodium falciparum malaria sporozoite invasion in primary cultures of human hepatocytes" @default.
• W2004402071 cites W139230029 @default.
• W2004402071 cites W1499308279 @default.
• W2004402071 cites W1526168422 @default.
• W2004402071 cites W1606423922 @default.
• W2004402071 cites W1931740302 @default.
• W2004402071 cites W1941199801 @default.
• W2004402071 cites W1969542805 @default.
• W2004402071 cites W1975224651 @default.
• W2004402071 cites W1989303926 @default.
• W2004402071 cites W1991236676 @default.
• W2004402071 cites W2006199737 @default.
• W2004402071 cites W2013742184 @default.
• W2004402071 cites W2029171425 @default.
• W2004402071 cites W2036887760 @default.
• W2004402071 cites W2045571578 @default.
• W2004402071 cites W2050065468 @default.
• W2004402071 cites W2051812340 @default.
• W2004402071 cites W2057457599 @default.
• W2004402071 cites W2063880266 @default.
• W2004402071 cites W2068593367 @default.
• W2004402071 cites W2073024879 @default.
• W2004402071 cites W2075810925 @default.
• W2004402071 cites W2101498837 @default.
• W2004402071 cites W2119825175 @default.
• W2004402071 cites W2155731278 @default.
• W2004402071 cites W2164320858 @default.
• W2004402071 cites W2321807405 @default.
• W2004402071 doi "https://doi.org/10.1016/s0168-8278(97)80086-4" @default.
• W2004402071 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9365045" @default.
• W2004402071 hasPublicationYear "1997" @default.
• W2004402071 type Work @default.
• W2004402071 sameAs 2004402071 @default.
• W2004402071 citedByCount "3" @default.
• W2004402071 crossrefType "journal-article" @default.
• W2004402071 hasAuthorship W2004402071A5000220586 @default.
• W2004402071 hasAuthorship W2004402071A5011594748 @default.
• W2004402071 hasAuthorship W2004402071A5021852823 @default.
• W2004402071 hasAuthorship W2004402071A5041185165 @default.
• W2004402071 hasAuthorship W2004402071A5080124451 @default.
• W2004402071 hasConcept C104317684 @default.
• W2004402071 hasConcept C153911025 @default.
• W2004402071 hasConcept C181199279 @default.
• W2004402071 hasConcept C187882448 @default.
• W2004402071 hasConcept C189482833 @default.
• W2004402071 hasConcept C202751555 @default.
• W2004402071 hasConcept C203014093 @default.
• W2004402071 hasConcept C2778048844 @default.
• W2004402071 hasConcept C2778325069 @default.
• W2004402071 hasConcept C2778371730 @default.
• W2004402071 hasConcept C40767141 @default.
• W2004402071 hasConcept C55493867 @default.
• W2004402071 hasConcept C86803240 @default.
• W2004402071 hasConceptScore W2004402071C104317684 @default.
• W2004402071 hasConceptScore W2004402071C153911025 @default.
• W2004402071 hasConceptScore W2004402071C181199279 @default.
• W2004402071 hasConceptScore W2004402071C187882448 @default.
• W2004402071 hasConceptScore W2004402071C189482833 @default.
• W2004402071 hasConceptScore W2004402071C202751555 @default.
• W2004402071 hasConceptScore W2004402071C203014093 @default.
• W2004402071 hasConceptScore W2004402071C2778048844 @default.
• W2004402071 hasConceptScore W2004402071C2778325069 @default.
• W2004402071 hasConceptScore W2004402071C2778371730 @default.
• W2004402071 hasConceptScore W2004402071C40767141 @default.
• W2004402071 hasConceptScore W2004402071C55493867 @default.
• W2004402071 hasConceptScore W2004402071C86803240 @default.
• W2004402071 hasIssue "4" @default.
• W2004402071 hasLocation W20044020711 @default.
• W2004402071 hasLocation W20044020712 @default.
• W2004402071 hasOpenAccess W2004402071 @default.
• W2004402071 hasPrimaryLocation W20044020711 @default.
• W2004402071 hasRelatedWork W1557866953 @default.
• W2004402071 hasRelatedWork W1981509814 @default.
• W2004402071 hasRelatedWork W2042689205 @default.
• W2004402071 hasRelatedWork W2051697791 @default.
• W2004402071 hasRelatedWork W2359741505 @default.
• W2004402071 hasRelatedWork W2361409233 @default.
• W2004402071 hasRelatedWork W2363550221 @default.
• W2004402071 hasRelatedWork W2382744586 @default.
• W2004402071 hasRelatedWork W2390750958 @default.
• W2004402071 hasRelatedWork W262341251 @default.
• W2004402071 hasVolume "27" @default.
• W2004402071 isParatext "false" @default.
• W2004402071 isRetracted "false" @default.
• W2004402071 magId "2004402071" @default.
• W2004402071 workType "article" @default.