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• W2004403763 abstract "Very few silver grains were seen on the luteal cell surface and none intracellularly after incubation for 2 h at 4 degrees C with 10 nM [3H]prostaglandin (PG) E1 or [3H]PGF2 alpha. However, incubation at 22 degrees C or 38 degrees C for 2 h resulted in association of numerous grains on the luteal cell surface as well as in several intracellular organelles. Qualitatively, the grain distribution was similar at 22 degrees C and 38 degrees C, but quantitatively there were fewer grains at 22 degrees C than at 38 degrees C. In addition to luteal cells, the grains were also found on erythrocytes, platelets, fibroblasts and capillary endothelial cells in luteal tissue incubated with [3H]PGE1. Grain association with non-luteal cells was never seen with [3H]PGF2 alpha. Coincubation of [3H]PGE1 or [3H]PGF2 alpha with only corresponding excess unlabeled PGs resulted in complete disappearance of silver grains from all the subcellular organelles. In autoradiographs, grains were seen on plasma membranes, nuclei, lysosomes, mitochondria, rough and smooth endoplasmic reticulum and Golgi. Three-step mask analysis, which effectively corrects for radiation spread, revealed that the grains found on mitochondria and rough endoplasmic reticulum in the case of [3H]PGE1, and those found on mitochondria, rough and smooth endoplasmic reticulum and Golgi in the case of [3H]PGF2 alpha, were due to the radiation spread. For both [3H]PGE1 and [3H]PGF2 alpha the plasma membrane associated grains progressively decreased, while the intracellular organelle associated grains increased with time at 38 degrees C. There were no grains in nuclei at 5 min but they appeared at 10 min and increased until 60 or 120 min. The optimized source density following 2 h of incubation at 38 degrees C with [3H]PGE1 was Golgi greater than plasma membranes greater than lysosomes = nuclei, and for [3H]PGF2 alpha, lysosomes greater than plasma membranes greater than nuclei. In summary, the present studies demonstrate for the first time that exogenously added [3H]PGE1 and [3H]PGF2 alpha internalize in bovine luteal cells in a ligand-specific, time- and temperature-dependent manner. The observations that the internalized [3H]PGE1 does not associate with rough endoplasmic reticulum and that [3H]PGF2 alpha does not associate with rough endoplasmic reticulum and Golgi, even though they contain the binding sites, suggest the presence of mechanism(s) to direct the internalized [3H]PGs to only certain intracellular organelles of luteal cells." @default.
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• W2004403763 date "1984-12-01" @default.
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• W2004403763 title "A quantitative electron microscope autoradiographic study on [3H]prostaglandin E1 and F2α internalization in bovine luteal slices" @default.
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• W2004403763 doi "https://doi.org/10.1016/0303-7207(84)90110-2" @default.
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