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- W2004403778 abstract "The kinetics of the hydrolysis of microcrystalline cellulose (MC) by a Trichoderma reesei cellulase complex and by the individual endoglucanase (pI 4.4–5.2) and cellobiohydrolase (pI 4.0–4.2) has been studied. A flow chart for the enzymatic hydrolysis of the cellulose has been revealed, which formed a basis for a computer simulation of the kinetic regularities observed. As a result of it, the values of the catalytic rate constants for the individual stages of the enzymatic degradation of MC have been calculated. Then, the synergistic behaviour of endoglucanase and cellobiohydrolase in the hydrolysis of MC has been described both quantitatively and graphically. The relative efficiency of the individual stages for the MC hydrolysis in terms of glucose and cellobiose formation for cellulase complexes of various composition has been calculated. It was quantitatively shown that cellobiohydrolase plays the key role in the MC hydrolysis by T. reesei cellulase preparations, because it gives up to 80% glucose and up to 80–90% cellobiose in the presnce of endoglucanase which in turn plays a relatively minor role in a direct formation of both soluble products of the hydrolysis." @default.
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- W2004403778 date "1986-01-01" @default.
- W2004403778 modified "2023-09-25" @default.
- W2004403778 title "Rolle der einzelnen Komponenten des Cellulasekomplexes bei der Hydrolyse unlöslicher Cellulose" @default.
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- W2004403778 doi "https://doi.org/10.1002/abio.370060412" @default.
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