FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004404328> ?p ?o ?g. }

• W2004404328 endingPage "509" @default.
• W2004404328 startingPage "498" @default.
• W2004404328 abstract "Normal and premalignant mouse mammary epithelial cells can be prepared in high yields by collagenase dissociation of minced glands followed by a brief, differential centrifugation to remove contaminating fibroblasts and fat cells. The major difficulties in preparing pure cultures in quantity are 1) incomplete dissociation of gland material, and 2) cell death during enzymatic digestion. These problems are eliminated by careful selection of collagenases for dissociation. Normal and premalignant mammary epithelial cells are morphologically indistinguishable from malignant mouse mammary epithelial cells in primary monolayer cultures. In addition, the growth rates and saturation densities achieved by normal mammary epithelial cells are indistinguishable from those of malignant mammary epithelial cells in primary culture. In both cases, a monolayer of cells is preserved with no evidence of focal overgrowth. Malignant adenocarcinoma mammary cells can however be distinguished from normal mammary epithelial cells by virtue of differences in their surface interactions with concanavalin A. A hemadsorption assay using Con-A-coated erythrocytes was the most sensitive indicator for these differences. In hemadsorption assays malignant mammary epithelial cells were half-maximally reactive with 2.5 mug/ml concanavalin A, while normal cells were completely unreactive even at concanavalin A concentrations five-times higher. Premalignant mammary epithelial cells were as reactive as malignant mammary epithelial cells in the hemadsorption assays. Hemadsorption of malignant cells was observed in primary and secondary cultures of epithelium as well as in cell lines. Malignant cells forming mammary adenocarcinomas were as highly reactive as malignant cells forming scirrhous carcinomas. Malignant cells not releasing mammary tumor virus (MuMTV) were as reactive as cells releasing that virus. Adsorption of concanavalin-A-coated erythrocytes to normal mammary epithelial cells could be induced by brief treatment of cell monolayers with hyaluronidase. Exposure of active sites was not affected with either trypsin or collagenase. Our results show that while the growth of malignant cells does not serve to distinguish them from normal cells in monolayer culture, surface changes do exist which can be identified by differences in concanavalin A reactivity. Since the earliest transformants identifiable in vivo (premalignant) have undergone conversion of the surface marker, concanavalin-A-mediated hemadsorption provides a sensitive measure for mammary epithelial cell transformants in vitro." @default.
• W2004404328 created "2016-06-24" @default.
• W2004404328 creator A5029111339 @default.
• W2004404328 creator A5073951984 @default.
• W2004404328 date "1976-10-15" @default.
• W2004404328 modified "2023-09-23" @default.
• W2004404328 title "Markers to distinguish normal and neoplastic mammary epithelial cellsin vitro: comparison of saturation density, morphology and concanavalin a reactivity" @default.
• W2004404328 cites W138879610 @default.
• W2004404328 cites W1496519404 @default.
• W2004404328 cites W1562654694 @default.
• W2004404328 cites W1563931024 @default.
• W2004404328 cites W1860479281 @default.
• W2004404328 cites W1981898671 @default.
• W2004404328 cites W1985493123 @default.
• W2004404328 cites W1987921977 @default.
• W2004404328 cites W1992018922 @default.
• W2004404328 cites W2003912814 @default.
• W2004404328 cites W2021823800 @default.
• W2004404328 cites W2056250532 @default.
• W2004404328 cites W2057340503 @default.
• W2004404328 cites W2061225879 @default.
• W2004404328 cites W2071997181 @default.
• W2004404328 cites W2077069390 @default.
• W2004404328 cites W2098481231 @default.
• W2004404328 cites W37133416 @default.
• W2004404328 doi "https://doi.org/10.1002/ijc.2910180415" @default.
• W2004404328 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/185159" @default.
• W2004404328 hasPublicationYear "1976" @default.
• W2004404328 type Work @default.
• W2004404328 sameAs 2004404328 @default.
• W2004404328 citedByCount "38" @default.
• W2004404328 crossrefType "journal-article" @default.
• W2004404328 hasAuthorship W2004404328A5029111339 @default.
• W2004404328 hasAuthorship W2004404328A5073951984 @default.
• W2004404328 hasConcept C121608353 @default.
• W2004404328 hasConcept C142724271 @default.
• W2004404328 hasConcept C153911025 @default.
• W2004404328 hasConcept C181199279 @default.
• W2004404328 hasConcept C185592680 @default.
• W2004404328 hasConcept C19869320 @default.
• W2004404328 hasConcept C202751555 @default.
• W2004404328 hasConcept C2776187077 @default.
• W2004404328 hasConcept C2778001805 @default.
• W2004404328 hasConcept C2778394429 @default.
• W2004404328 hasConcept C529295009 @default.
• W2004404328 hasConcept C530470458 @default.
• W2004404328 hasConcept C54355233 @default.
• W2004404328 hasConcept C55493867 @default.
• W2004404328 hasConcept C71924100 @default.
• W2004404328 hasConcept C81885089 @default.
• W2004404328 hasConcept C86803240 @default.
• W2004404328 hasConceptScore W2004404328C121608353 @default.
• W2004404328 hasConceptScore W2004404328C142724271 @default.
• W2004404328 hasConceptScore W2004404328C153911025 @default.
• W2004404328 hasConceptScore W2004404328C181199279 @default.
• W2004404328 hasConceptScore W2004404328C185592680 @default.
• W2004404328 hasConceptScore W2004404328C19869320 @default.
• W2004404328 hasConceptScore W2004404328C202751555 @default.
• W2004404328 hasConceptScore W2004404328C2776187077 @default.
• W2004404328 hasConceptScore W2004404328C2778001805 @default.
• W2004404328 hasConceptScore W2004404328C2778394429 @default.
• W2004404328 hasConceptScore W2004404328C529295009 @default.
• W2004404328 hasConceptScore W2004404328C530470458 @default.
• W2004404328 hasConceptScore W2004404328C54355233 @default.
• W2004404328 hasConceptScore W2004404328C55493867 @default.
• W2004404328 hasConceptScore W2004404328C71924100 @default.
• W2004404328 hasConceptScore W2004404328C81885089 @default.
• W2004404328 hasConceptScore W2004404328C86803240 @default.
• W2004404328 hasIssue "4" @default.
• W2004404328 hasLocation W20044043281 @default.
• W2004404328 hasLocation W20044043282 @default.
• W2004404328 hasOpenAccess W2004404328 @default.
• W2004404328 hasPrimaryLocation W20044043281 @default.
• W2004404328 hasRelatedWork W1519012750 @default.
• W2004404328 hasRelatedWork W1531014903 @default.
• W2004404328 hasRelatedWork W2004404328 @default.
• W2004404328 hasRelatedWork W2025217354 @default.
• W2004404328 hasRelatedWork W2058562326 @default.
• W2004404328 hasRelatedWork W2354201022 @default.
• W2004404328 hasRelatedWork W2417838062 @default.
• W2004404328 hasRelatedWork W2462582443 @default.
• W2004404328 hasRelatedWork W2500314303 @default.
• W2004404328 hasRelatedWork W4289992199 @default.
• W2004404328 hasVolume "18" @default.
• W2004404328 isParatext "false" @default.
• W2004404328 isRetracted "false" @default.
• W2004404328 magId "2004404328" @default.
• W2004404328 workType "article" @default.