FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004407614> ?p ?o ?g. }

• W2004407614 endingPage "35154" @default.
• W2004407614 startingPage "35142" @default.
• W2004407614 abstract "In Saccharomyces cerevisiae, silent chromatin is formed at HMR upon the passage through S phase, yet neither the initiation of DNA replication at silencers nor the passage of a replication fork through HMR is required for silencing. Paradoxically, mutations in the DNA replication processivity factor, POL30, disrupt silencing despite this lack of requirement for DNA replication in the establishment of silencing. We tested whether pol30 mutants could establish silencing at either replicated or non-replicated HMR loci during S phase and found that pol30 mutants were defective in establishing silencing at HMR regardless of its replication status. Although previous studies tie the silencing defect of pol30 mutants to the chromatin assembly factors Asf1p and CAF-1, we found pol30 mutants did not exhibit a gross defect in packaging HMR into chromatin. Rather, the pol30 mutants exhibited defects in histone modifications linked to ASF1 and CAF-1-dependent pathways, including SAS-I- and Rtt109p-dependent acetylation events at H4-K16 and H3-K9 (plus H3-K56; Miller, A., Yang, B., Foster, T., and Kirchmaier, A. L. (2008) Genetics 179, 793–809). Additional experiments using FLIM-FRET revealed that Pol30p interacted with SAS-I and Rtt109p in the nuclei of living cells. However, these interactions were disrupted in pol30 mutants with defects linked to ASF1- and CAF-1-dependent pathways. Together, these results imply that Pol30p affects epigenetic processes by influencing the composition of chromosomal histone modifications. In Saccharomyces cerevisiae, silent chromatin is formed at HMR upon the passage through S phase, yet neither the initiation of DNA replication at silencers nor the passage of a replication fork through HMR is required for silencing. Paradoxically, mutations in the DNA replication processivity factor, POL30, disrupt silencing despite this lack of requirement for DNA replication in the establishment of silencing. We tested whether pol30 mutants could establish silencing at either replicated or non-replicated HMR loci during S phase and found that pol30 mutants were defective in establishing silencing at HMR regardless of its replication status. Although previous studies tie the silencing defect of pol30 mutants to the chromatin assembly factors Asf1p and CAF-1, we found pol30 mutants did not exhibit a gross defect in packaging HMR into chromatin. Rather, the pol30 mutants exhibited defects in histone modifications linked to ASF1 and CAF-1-dependent pathways, including SAS-I- and Rtt109p-dependent acetylation events at H4-K16 and H3-K9 (plus H3-K56; Miller, A., Yang, B., Foster, T., and Kirchmaier, A. L. (2008) Genetics 179, 793–809). Additional experiments using FLIM-FRET revealed that Pol30p interacted with SAS-I and Rtt109p in the nuclei of living cells. However, these interactions were disrupted in pol30 mutants with defects linked to ASF1- and CAF-1-dependent pathways. Together, these results imply that Pol30p affects epigenetic processes by influencing the composition of chromosomal histone modifications." @default.
• W2004407614 created "2016-06-24" @default.
• W2004407614 creator A5009385854 @default.
• W2004407614 creator A5011077455 @default.
• W2004407614 creator A5050356076 @default.
• W2004407614 creator A5053469136 @default.
• W2004407614 creator A5066925673 @default.
• W2004407614 creator A5084659336 @default.
• W2004407614 creator A5085585684 @default.
• W2004407614 date "2010-11-01" @default.
• W2004407614 modified "2023-09-30" @default.
• W2004407614 title "Proliferating Cell Nuclear Antigen (PCNA) Is Required for Cell Cycle-regulated Silent Chromatin on Replicated and Nonreplicated Genes" @default.
• W2004407614 cites W1487365152 @default.
• W2004407614 cites W1501743111 @default.
• W2004407614 cites W1512281455 @default.
• W2004407614 cites W1519860621 @default.
• W2004407614 cites W1533942477 @default.
• W2004407614 cites W1550854352 @default.
• W2004407614 cites W1904065091 @default.
• W2004407614 cites W1906365906 @default.
• W2004407614 cites W1908340894 @default.
• W2004407614 cites W1908345703 @default.
• W2004407614 cites W1965787451 @default.
• W2004407614 cites W1965975821 @default.
• W2004407614 cites W1966698614 @default.
• W2004407614 cites W1968002116 @default.
• W2004407614 cites W1969543649 @default.
• W2004407614 cites W1970304959 @default.
• W2004407614 cites W1972345616 @default.
• W2004407614 cites W1975091455 @default.
• W2004407614 cites W1975425948 @default.
• W2004407614 cites W1975939844 @default.
• W2004407614 cites W1977642285 @default.
• W2004407614 cites W1985634754 @default.
• W2004407614 cites W1989849863 @default.
• W2004407614 cites W1990103859 @default.
• W2004407614 cites W1992980647 @default.
• W2004407614 cites W1994245744 @default.
• W2004407614 cites W1997859335 @default.
• W2004407614 cites W1999031485 @default.
• W2004407614 cites W2006225938 @default.
• W2004407614 cites W2006901859 @default.
• W2004407614 cites W2009238064 @default.
• W2004407614 cites W2010817679 @default.
• W2004407614 cites W2012765618 @default.
• W2004407614 cites W2015529578 @default.
• W2004407614 cites W2015669100 @default.
• W2004407614 cites W2016546870 @default.
• W2004407614 cites W2017230219 @default.
• W2004407614 cites W2020763705 @default.
• W2004407614 cites W2029440030 @default.
• W2004407614 cites W2031481332 @default.
• W2004407614 cites W2031918634 @default.
• W2004407614 cites W2037036397 @default.
• W2004407614 cites W2039564969 @default.
• W2004407614 cites W2039940539 @default.
• W2004407614 cites W2040323551 @default.
• W2004407614 cites W2041674913 @default.
• W2004407614 cites W2043035057 @default.
• W2004407614 cites W2046894450 @default.
• W2004407614 cites W2049341559 @default.
• W2004407614 cites W2049984581 @default.
• W2004407614 cites W2052389165 @default.
• W2004407614 cites W2052540561 @default.
• W2004407614 cites W2053213954 @default.
• W2004407614 cites W2053289821 @default.
• W2004407614 cites W2054881393 @default.
• W2004407614 cites W2055171335 @default.
• W2004407614 cites W2056550647 @default.
• W2004407614 cites W2056631693 @default.
• W2004407614 cites W2060496135 @default.
• W2004407614 cites W2069233620 @default.
• W2004407614 cites W2071955851 @default.
• W2004407614 cites W2072747624 @default.
• W2004407614 cites W2075230261 @default.
• W2004407614 cites W2075549762 @default.
• W2004407614 cites W2080756244 @default.
• W2004407614 cites W2080895949 @default.
• W2004407614 cites W2083366213 @default.
• W2004407614 cites W2085216777 @default.
• W2004407614 cites W2088853419 @default.
• W2004407614 cites W2093189184 @default.
• W2004407614 cites W2093220039 @default.
• W2004407614 cites W2094051937 @default.
• W2004407614 cites W2095059074 @default.
• W2004407614 cites W2098821915 @default.
• W2004407614 cites W2100057937 @default.
• W2004407614 cites W2100941051 @default.
• W2004407614 cites W2105487944 @default.
• W2004407614 cites W2107168601 @default.
• W2004407614 cites W2108116072 @default.
• W2004407614 cites W2109546612 @default.
• W2004407614 cites W2111166855 @default.
• W2004407614 cites W2111217339 @default.
• W2004407614 cites W2115838795 @default.
• W2004407614 cites W2118316527 @default.
• W2004407614 cites W2119091673 @default.
• W2004407614 cites W2119175799 @default.

• next