FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004421590> ?p ?o ?g. }

• W2004421590 endingPage "291" @default.
• W2004421590 startingPage "280" @default.
• W2004421590 abstract "The multiparameter fluorometric analysis of intact and fixed cells often requires the use of a nuclear DNA discrimination signal with spectral separation from visible range fluorochromes. We have developed a novel deep red fluorescing bisalkylaminoanthraquinone, DRAQ5 (Ex(lambdamax) 646 nm; Em(lambdamax) 681 nm; Em(lambdarange) 665->800 nm), with high affinity for DNA and a high capacity to enter living cells. We describe here the spectral characteristics and applications of this synthetic compound, particularly in relation to cytometric analysis of the cell cycle.Cultured human tumor cells were examined for the ability to nuclear locate DRAQ5 using single and multiphoton laser scanning microscopy (LSM) and multiparameter flow cytometry.Multiparameter flow cytometry shows that the dye can rapidly report the cellular DNA content of live and fixed cells at a resolution level adequate for cell cycle analysis and the cycle-specific expression of cellular proteins (e.g., cyclin B1). The preferential excitation of DRAQ5 by laser red lines (633/647 nm) was found to offer a means of fluorescence signal discrimination by selective excitation, with greatly reduced emission overlap with UV-excitable and visible range fluophors as compared with propidium iodide. LSM reveals nuclear architecture and clearly defines chromosomal elements in live cells. DRAQ5 was found to permit multiphoton imaging of nuclei using a 1,047-nm emitting mode-locked YLF laser. The unusual spectral properties of DRAQ5 also permit live cell DNA analysis using conventional 488 nm excitation and the single-photon imaging of nuclear fluorescence using laser excitation between 488 nm and low infrared (IR; 780 nm) wavelengths. Single and multiphoton microscopy studies revealed the ability of DRAQ5 to report three-dimensional nuclear structure and location in live cells expressing endoplasmic reticulum targeted-GFP, MitoTracker-stained mitochondria, or a vital cell probe for free zinc (Zinquin).The fluorescence excitation and emission characteristics of DRAQ5 in living and fixed cells permit the incorporation of the measurement of cellular DNA content into a variety of multiparameter cytometric analyses." @default.
• W2004421590 created "2016-06-24" @default.
• W2004421590 creator A5010097977 @default.
• W2004421590 creator A5023034399 @default.
• W2004421590 creator A5024402063 @default.
• W2004421590 creator A5024864543 @default.
• W2004421590 creator A5057238208 @default.
• W2004421590 creator A5064184281 @default.
• W2004421590 creator A5069019211 @default.
• W2004421590 creator A5081902236 @default.
• W2004421590 creator A5086245744 @default.
• W2004421590 creator A5089860172 @default.
• W2004421590 date "2000-01-01" @default.
• W2004421590 modified "2023-10-11" @default.
• W2004421590 title "Characteristics of a novel deep red/infrared fluorescent cell-permeant DNA probe, DRAQ5, in intact human cells analyzed by flow cytometry, confocal and multiphoton microscopy" @default.
• W2004421590 cites W1552946383 @default.
• W2004421590 cites W1916482672 @default.
• W2004421590 cites W1972957002 @default.
• W2004421590 cites W1974131180 @default.
• W2004421590 cites W1997843453 @default.
• W2004421590 cites W2006986478 @default.
• W2004421590 cites W2019351052 @default.
• W2004421590 cites W2038512880 @default.
• W2004421590 cites W2039010642 @default.
• W2004421590 cites W2042786079 @default.
• W2004421590 cites W2046588938 @default.
• W2004421590 cites W2048212315 @default.
• W2004421590 cites W2051862853 @default.
• W2004421590 cites W2061464631 @default.
• W2004421590 cites W2067507228 @default.
• W2004421590 cites W2081407910 @default.
• W2004421590 cites W2088911899 @default.
• W2004421590 cites W2096880268 @default.
• W2004421590 cites W2127264662 @default.
• W2004421590 cites W2144737712 @default.
• W2004421590 doi "https://doi.org/10.1002/1097-0320(20000801)40:4<280::aid-cyto4>3.0.co;2-7" @default.
• W2004421590 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10918279" @default.
• W2004421590 hasPublicationYear "2000" @default.
• W2004421590 type Work @default.
• W2004421590 sameAs 2004421590 @default.
• W2004421590 citedByCount "148" @default.
• W2004421590 countsByYear W20044215902012 @default.
• W2004421590 countsByYear W20044215902013 @default.
• W2004421590 countsByYear W20044215902014 @default.
• W2004421590 countsByYear W20044215902015 @default.
• W2004421590 countsByYear W20044215902016 @default.
• W2004421590 countsByYear W20044215902017 @default.
• W2004421590 countsByYear W20044215902018 @default.
• W2004421590 countsByYear W20044215902019 @default.
• W2004421590 countsByYear W20044215902020 @default.
• W2004421590 countsByYear W20044215902021 @default.
• W2004421590 countsByYear W20044215902022 @default.
• W2004421590 countsByYear W20044215902023 @default.
• W2004421590 crossrefType "journal-article" @default.
• W2004421590 hasAuthorship W2004421590A5010097977 @default.
• W2004421590 hasAuthorship W2004421590A5023034399 @default.
• W2004421590 hasAuthorship W2004421590A5024402063 @default.
• W2004421590 hasAuthorship W2004421590A5024864543 @default.
• W2004421590 hasAuthorship W2004421590A5057238208 @default.
• W2004421590 hasAuthorship W2004421590A5064184281 @default.
• W2004421590 hasAuthorship W2004421590A5069019211 @default.
• W2004421590 hasAuthorship W2004421590A5081902236 @default.
• W2004421590 hasAuthorship W2004421590A5086245744 @default.
• W2004421590 hasAuthorship W2004421590A5089860172 @default.
• W2004421590 hasBestOaLocation W20044215901 @default.
• W2004421590 hasConcept C120665830 @default.
• W2004421590 hasConcept C121332964 @default.
• W2004421590 hasConcept C12554922 @default.
• W2004421590 hasConcept C136009344 @default.
• W2004421590 hasConcept C147080431 @default.
• W2004421590 hasConcept C153911025 @default.
• W2004421590 hasConcept C169274487 @default.
• W2004421590 hasConcept C185592680 @default.
• W2004421590 hasConcept C190283241 @default.
• W2004421590 hasConcept C192562407 @default.
• W2004421590 hasConcept C2775934118 @default.
• W2004421590 hasConcept C2780339063 @default.
• W2004421590 hasConcept C31573885 @default.
• W2004421590 hasConcept C520434653 @default.
• W2004421590 hasConcept C553184892 @default.
• W2004421590 hasConcept C55493867 @default.
• W2004421590 hasConcept C86803240 @default.
• W2004421590 hasConcept C91881484 @default.
• W2004421590 hasConceptScore W2004421590C120665830 @default.
• W2004421590 hasConceptScore W2004421590C121332964 @default.
• W2004421590 hasConceptScore W2004421590C12554922 @default.
• W2004421590 hasConceptScore W2004421590C136009344 @default.
• W2004421590 hasConceptScore W2004421590C147080431 @default.
• W2004421590 hasConceptScore W2004421590C153911025 @default.
• W2004421590 hasConceptScore W2004421590C169274487 @default.
• W2004421590 hasConceptScore W2004421590C185592680 @default.
• W2004421590 hasConceptScore W2004421590C190283241 @default.
• W2004421590 hasConceptScore W2004421590C192562407 @default.
• W2004421590 hasConceptScore W2004421590C2775934118 @default.
• W2004421590 hasConceptScore W2004421590C2780339063 @default.
• W2004421590 hasConceptScore W2004421590C31573885 @default.
• W2004421590 hasConceptScore W2004421590C520434653 @default.
• W2004421590 hasConceptScore W2004421590C553184892 @default.

• next