Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004426188> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2004426188 abstract "The hydrogenase from Paracoccus denitrificans is an integral membrane protein and has been solubilised by Triton X-100. The membrane-bound and detergent-solubilised forms of the enzyme have been compared. Both forms of the enzyme show a pH optimum for reduction of benzyl viologen at pH 8.5--9.0 and are both inhibited by concentrations of NaCl greater than 30 mM. An Arrhenius plot of the activity of hydrogenase in the membrane shows no 'break'. The form of the Arrhenius plot and the activation energy are not significantly changed on solubilisation of the enzyme. The Km and V values for benzyl viologen, methyl viologen and H2 are unaltered when the enzyme is extracted from the membrane. Therefore, solubilisation of hydrogenase from the membrane by Triton X-400 is unlikely to disrupt the native conformation of the enzyme. The detergent-solubilised hydrogenase has subsequently been purified using ammonium sulphate precipitation, sucrose density gradient centrifugation and chromatography on hydroxyapatite. The overall yield of activity is 23%, with a final purification of over 100-fold." @default.
- W2004426188 created "2016-06-24" @default.
- W2004426188 creator A5050853948 @default.
- W2004426188 creator A5071196257 @default.
- W2004426188 date "1979-09-01" @default.
- W2004426188 modified "2023-09-26" @default.
- W2004426188 title "Comparison of the membrane-bound and detergent-solubilised hydrogenase from Paracoccus denitrificans. Isolation of the hydrogenase" @default.
- W2004426188 cites W1625660792 @default.
- W2004426188 cites W171854198 @default.
- W2004426188 cites W1771014981 @default.
- W2004426188 cites W1775749144 @default.
- W2004426188 cites W1979204404 @default.
- W2004426188 cites W1983545215 @default.
- W2004426188 cites W1985212636 @default.
- W2004426188 cites W1993425013 @default.
- W2004426188 cites W2011219512 @default.
- W2004426188 cites W2012458218 @default.
- W2004426188 cites W2018464181 @default.
- W2004426188 cites W2024187309 @default.
- W2004426188 cites W2033528007 @default.
- W2004426188 cites W2039387536 @default.
- W2004426188 cites W2067489859 @default.
- W2004426188 cites W2084856012 @default.
- W2004426188 cites W2085526532 @default.
- W2004426188 cites W2090764568 @default.
- W2004426188 cites W2090846303 @default.
- W2004426188 cites W2092861677 @default.
- W2004426188 cites W2093645948 @default.
- W2004426188 cites W2134386404 @default.
- W2004426188 cites W2219009340 @default.
- W2004426188 cites W2323431806 @default.
- W2004426188 cites W3024817344 @default.
- W2004426188 cites W57615732 @default.
- W2004426188 doi "https://doi.org/10.1016/0005-2744(79)90199-2" @default.
- W2004426188 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/39613" @default.
- W2004426188 hasPublicationYear "1979" @default.
- W2004426188 type Work @default.
- W2004426188 sameAs 2004426188 @default.
- W2004426188 citedByCount "24" @default.
- W2004426188 countsByYear W20044261882019 @default.
- W2004426188 crossrefType "journal-article" @default.
- W2004426188 hasAuthorship W2004426188A5050853948 @default.
- W2004426188 hasAuthorship W2004426188A5071196257 @default.
- W2004426188 hasConcept C178790620 @default.
- W2004426188 hasConcept C181199279 @default.
- W2004426188 hasConcept C185592680 @default.
- W2004426188 hasConcept C2780640746 @default.
- W2004426188 hasConcept C41625074 @default.
- W2004426188 hasConcept C43617362 @default.
- W2004426188 hasConcept C55493867 @default.
- W2004426188 hasConcept C79115784 @default.
- W2004426188 hasConcept C8739425 @default.
- W2004426188 hasConcept C95121573 @default.
- W2004426188 hasConceptScore W2004426188C178790620 @default.
- W2004426188 hasConceptScore W2004426188C181199279 @default.
- W2004426188 hasConceptScore W2004426188C185592680 @default.
- W2004426188 hasConceptScore W2004426188C2780640746 @default.
- W2004426188 hasConceptScore W2004426188C41625074 @default.
- W2004426188 hasConceptScore W2004426188C43617362 @default.
- W2004426188 hasConceptScore W2004426188C55493867 @default.
- W2004426188 hasConceptScore W2004426188C79115784 @default.
- W2004426188 hasConceptScore W2004426188C8739425 @default.
- W2004426188 hasConceptScore W2004426188C95121573 @default.
- W2004426188 hasLocation W20044261881 @default.
- W2004426188 hasLocation W20044261882 @default.
- W2004426188 hasOpenAccess W2004426188 @default.
- W2004426188 hasPrimaryLocation W20044261881 @default.
- W2004426188 hasRelatedWork W1485511193 @default.
- W2004426188 hasRelatedWork W1976274559 @default.
- W2004426188 hasRelatedWork W1982099523 @default.
- W2004426188 hasRelatedWork W2004426188 @default.
- W2004426188 hasRelatedWork W2012311798 @default.
- W2004426188 hasRelatedWork W2054859837 @default.
- W2004426188 hasRelatedWork W2061173555 @default.
- W2004426188 hasRelatedWork W2084012522 @default.
- W2004426188 hasRelatedWork W2096265168 @default.
- W2004426188 hasRelatedWork W2324195831 @default.
- W2004426188 isParatext "false" @default.
- W2004426188 isRetracted "false" @default.
- W2004426188 magId "2004426188" @default.
- W2004426188 workType "article" @default.