FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004451962> ?p ?o ?g. }

• W2004451962 endingPage "16" @default.
• W2004451962 startingPage "1" @default.
• W2004451962 abstract "Recently steady-state and picosecond time-resolved absorption and fluorescence spectroscopy on the Green Fluorescent Protein (GFP) have been interpreted by a mechanism where the key process is an excited state deprotonation of the chromophore (M. Chattoraij, B.A. King, G.U. Bublitz and S.G. Boxer, Proc. Natl. Acad. Sci. USA, 93 (1996) 8362–8367). Such a conclusion was borne out by the mirror image of the picosecond decay of the protonated species RH∗ in the blue and the concomitant picosecond rise of the green fluorescence of the deprotonated fluorophore R−∗ as well as the significant slowing of both kinetic features upon deuteration. We report similar experiments confirming this mechanism. The results of ultrafast spectroscopy on wild-type GFP together with two important mutants combined with the recent crystal structures are shown to shed more light on the interplay between absorption and emission phenomena in GFP. Beyond some differences with previous results pertaining, for instance, to the assignment of vibronic progressions in absorption spectra and the temperature dependence of excited state deprotonation, several new features have been identified. These concern the deprotonated ground state R− in equilibrium as well as the excited state RH∗. In particular, we have studied the distributed fluorescence kinetics in the time and frequency domain, excited state absorption features observed in femtosecond time-resolution, and the dependence of excited state proton transfer kinetics on the aggregational state of the protein." @default.
• W2004451962 created "2016-06-24" @default.
• W2004451962 creator A5001541556 @default.
• W2004451962 creator A5003053308 @default.
• W2004451962 creator A5003066326 @default.
• W2004451962 creator A5006274809 @default.
• W2004451962 creator A5015433124 @default.
• W2004451962 creator A5016623498 @default.
• W2004451962 creator A5017823242 @default.
• W2004451962 creator A5022716368 @default.
• W2004451962 creator A5027728751 @default.
• W2004451962 creator A5030880894 @default.
• W2004451962 creator A5042600304 @default.
• W2004451962 date "1996-12-01" @default.
• W2004451962 modified "2023-10-12" @default.
• W2004451962 title "Time-resolved spectroscopy of wild-type and mutant Green Fluorescent Proteins reveals excited state deprotonation consistent with fluorophore-protein interactions" @default.
• W2004451962 cites W1482470973 @default.
• W2004451962 cites W1982808369 @default.
• W2004451962 cites W2001950012 @default.
• W2004451962 cites W2009451766 @default.
• W2004451962 cites W2014293907 @default.
• W2004451962 cites W2024624698 @default.
• W2004451962 cites W2030695915 @default.
• W2004451962 cites W2058254034 @default.
• W2004451962 cites W2074519855 @default.
• W2004451962 cites W2085954482 @default.
• W2004451962 cites W2090672719 @default.
• W2004451962 cites W2119612945 @default.
• W2004451962 cites W2145049033 @default.
• W2004451962 cites W2161996750 @default.
• W2004451962 cites W2315188920 @default.
• W2004451962 cites W4233808990 @default.
• W2004451962 cites W4251698042 @default.
• W2004451962 doi "https://doi.org/10.1016/s0301-0104(96)00340-0" @default.
• W2004451962 hasPublicationYear "1996" @default.
• W2004451962 type Work @default.
• W2004451962 sameAs 2004451962 @default.
• W2004451962 citedByCount "232" @default.
• W2004451962 countsByYear W20044519622012 @default.
• W2004451962 countsByYear W20044519622013 @default.
• W2004451962 countsByYear W20044519622014 @default.
• W2004451962 countsByYear W20044519622015 @default.
• W2004451962 countsByYear W20044519622016 @default.
• W2004451962 countsByYear W20044519622017 @default.
• W2004451962 countsByYear W20044519622018 @default.
• W2004451962 countsByYear W20044519622019 @default.
• W2004451962 countsByYear W20044519622020 @default.
• W2004451962 countsByYear W20044519622021 @default.
• W2004451962 countsByYear W20044519622022 @default.
• W2004451962 countsByYear W20044519622023 @default.
• W2004451962 crossrefType "journal-article" @default.
• W2004451962 hasAuthorship W2004451962A5001541556 @default.
• W2004451962 hasAuthorship W2004451962A5003053308 @default.
• W2004451962 hasAuthorship W2004451962A5003066326 @default.
• W2004451962 hasAuthorship W2004451962A5006274809 @default.
• W2004451962 hasAuthorship W2004451962A5015433124 @default.
• W2004451962 hasAuthorship W2004451962A5016623498 @default.
• W2004451962 hasAuthorship W2004451962A5017823242 @default.
• W2004451962 hasAuthorship W2004451962A5022716368 @default.
• W2004451962 hasAuthorship W2004451962A5027728751 @default.
• W2004451962 hasAuthorship W2004451962A5030880894 @default.
• W2004451962 hasAuthorship W2004451962A5042600304 @default.
• W2004451962 hasConcept C118629725 @default.
• W2004451962 hasConcept C119824511 @default.
• W2004451962 hasConcept C120665830 @default.
• W2004451962 hasConcept C121332964 @default.
• W2004451962 hasConcept C125287762 @default.
• W2004451962 hasConcept C133279788 @default.
• W2004451962 hasConcept C145148216 @default.
• W2004451962 hasConcept C155220765 @default.
• W2004451962 hasConcept C158711907 @default.
• W2004451962 hasConcept C178790620 @default.
• W2004451962 hasConcept C181500209 @default.
• W2004451962 hasConcept C184779094 @default.
• W2004451962 hasConcept C185592680 @default.
• W2004451962 hasConcept C192468462 @default.
• W2004451962 hasConcept C2778617687 @default.
• W2004451962 hasConcept C32891209 @default.
• W2004451962 hasConcept C520434653 @default.
• W2004451962 hasConcept C55005982 @default.
• W2004451962 hasConcept C62520636 @default.
• W2004451962 hasConcept C69523127 @default.
• W2004451962 hasConcept C75473681 @default.
• W2004451962 hasConcept C91881484 @default.
• W2004451962 hasConcept C95503338 @default.
• W2004451962 hasConceptScore W2004451962C118629725 @default.
• W2004451962 hasConceptScore W2004451962C119824511 @default.
• W2004451962 hasConceptScore W2004451962C120665830 @default.
• W2004451962 hasConceptScore W2004451962C121332964 @default.
• W2004451962 hasConceptScore W2004451962C125287762 @default.
• W2004451962 hasConceptScore W2004451962C133279788 @default.
• W2004451962 hasConceptScore W2004451962C145148216 @default.
• W2004451962 hasConceptScore W2004451962C155220765 @default.
• W2004451962 hasConceptScore W2004451962C158711907 @default.
• W2004451962 hasConceptScore W2004451962C178790620 @default.
• W2004451962 hasConceptScore W2004451962C181500209 @default.
• W2004451962 hasConceptScore W2004451962C184779094 @default.
• W2004451962 hasConceptScore W2004451962C185592680 @default.

• next