Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004452302> ?p ?o ?g. }
- W2004452302 endingPage "7412" @default.
- W2004452302 startingPage "7406" @default.
- W2004452302 abstract "Due to their short lifespan, rapid division, and ease of genetic manipulation, yeasts are popular model organisms for studying aging in actively dividing cells. To study replicative aging over many cell divisions, individual cells must be continuously separated from their progeny via a laborious manual microdissection procedure. Microfluidics-based soft-lithography devices have recently been used to automate microdissection of the budding yeast Saccharomyces cerevisiae. However, little is known about replicative aging in Schizosaccharomyces pombe, a rod-shaped yeast that divides by binary fission and shares many conserved biological functions with higher eukaryotes. In this report, we develop a versatile multiphoton lithography method that enables rapid fabrication of three-dimensional master structures for polydimethylsiloxane (PDMS)-based microfluidics. We exploit the rapid prototyping capabilities of multiphoton lithography to create and characterize a cell-capture device that is capable of high-resolution microscopic observation of hundreds of individual S. pombe cells. By continuously removing the progeny cells, we demonstrate that cell growth and protein aggregation can be tracked in individual cells for over ∼100 h. Thus, the fission yeast lifespan microdissector (FYLM) provides a powerful on-chip microdissection platform that will enable high-throughput studies of aging in rod-shaped cells." @default.
- W2004452302 created "2016-06-24" @default.
- W2004452302 creator A5002636771 @default.
- W2004452302 creator A5065207206 @default.
- W2004452302 creator A5077327911 @default.
- W2004452302 creator A5080401389 @default.
- W2004452302 date "2014-07-17" @default.
- W2004452302 modified "2023-10-14" @default.
- W2004452302 title "3D-Printed Microfluidic Microdissector for High-Throughput Studies of Cellular Aging" @default.
- W2004452302 cites W1539010571 @default.
- W2004452302 cites W1964411676 @default.
- W2004452302 cites W1966041584 @default.
- W2004452302 cites W1966989737 @default.
- W2004452302 cites W1974632597 @default.
- W2004452302 cites W1977031005 @default.
- W2004452302 cites W1985793906 @default.
- W2004452302 cites W1992663222 @default.
- W2004452302 cites W1994980407 @default.
- W2004452302 cites W1996666978 @default.
- W2004452302 cites W1996902029 @default.
- W2004452302 cites W2005037691 @default.
- W2004452302 cites W2013182567 @default.
- W2004452302 cites W2031476233 @default.
- W2004452302 cites W2045441603 @default.
- W2004452302 cites W2049292941 @default.
- W2004452302 cites W2053404809 @default.
- W2004452302 cites W2054826729 @default.
- W2004452302 cites W2058238342 @default.
- W2004452302 cites W2058462185 @default.
- W2004452302 cites W2058484936 @default.
- W2004452302 cites W2066837250 @default.
- W2004452302 cites W2067940580 @default.
- W2004452302 cites W2068625350 @default.
- W2004452302 cites W2074467340 @default.
- W2004452302 cites W2075680988 @default.
- W2004452302 cites W2075762672 @default.
- W2004452302 cites W2080436516 @default.
- W2004452302 cites W2090572943 @default.
- W2004452302 cites W2105105960 @default.
- W2004452302 cites W2105375182 @default.
- W2004452302 cites W2106069844 @default.
- W2004452302 cites W2114040677 @default.
- W2004452302 cites W2121020843 @default.
- W2004452302 cites W2132296982 @default.
- W2004452302 cites W2134090641 @default.
- W2004452302 cites W2135532366 @default.
- W2004452302 cites W2135855342 @default.
- W2004452302 cites W2140354820 @default.
- W2004452302 cites W2149787945 @default.
- W2004452302 cites W2153051494 @default.
- W2004452302 cites W2156684470 @default.
- W2004452302 cites W2163871471 @default.
- W2004452302 cites W2168108067 @default.
- W2004452302 cites W2170940406 @default.
- W2004452302 cites W4247419735 @default.
- W2004452302 cites W4319290968 @default.
- W2004452302 doi "https://doi.org/10.1021/ac500893a" @default.
- W2004452302 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4636036" @default.
- W2004452302 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24992972" @default.
- W2004452302 hasPublicationYear "2014" @default.
- W2004452302 type Work @default.
- W2004452302 sameAs 2004452302 @default.
- W2004452302 citedByCount "48" @default.
- W2004452302 countsByYear W20044523022015 @default.
- W2004452302 countsByYear W20044523022016 @default.
- W2004452302 countsByYear W20044523022017 @default.
- W2004452302 countsByYear W20044523022018 @default.
- W2004452302 countsByYear W20044523022019 @default.
- W2004452302 countsByYear W20044523022020 @default.
- W2004452302 countsByYear W20044523022021 @default.
- W2004452302 countsByYear W20044523022022 @default.
- W2004452302 countsByYear W20044523022023 @default.
- W2004452302 crossrefType "journal-article" @default.
- W2004452302 hasAuthorship W2004452302A5002636771 @default.
- W2004452302 hasAuthorship W2004452302A5065207206 @default.
- W2004452302 hasAuthorship W2004452302A5077327911 @default.
- W2004452302 hasAuthorship W2004452302A5080401389 @default.
- W2004452302 hasBestOaLocation W20044523022 @default.
- W2004452302 hasConcept C104317684 @default.
- W2004452302 hasConcept C11242284 @default.
- W2004452302 hasConcept C157764524 @default.
- W2004452302 hasConcept C171250308 @default.
- W2004452302 hasConcept C177336024 @default.
- W2004452302 hasConcept C185592680 @default.
- W2004452302 hasConcept C192562407 @default.
- W2004452302 hasConcept C204223013 @default.
- W2004452302 hasConcept C2776519988 @default.
- W2004452302 hasConcept C2777576037 @default.
- W2004452302 hasConcept C2779222958 @default.
- W2004452302 hasConcept C2779429346 @default.
- W2004452302 hasConcept C2779849746 @default.
- W2004452302 hasConcept C2908780919 @default.
- W2004452302 hasConcept C2992884146 @default.
- W2004452302 hasConcept C41008148 @default.
- W2004452302 hasConcept C49040817 @default.
- W2004452302 hasConcept C552990157 @default.
- W2004452302 hasConcept C55493867 @default.
- W2004452302 hasConcept C555944384 @default.