FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004452311> ?p ?o ?g. }

• W2004452311 endingPage "6635" @default.
• W2004452311 startingPage "6629" @default.
• W2004452311 abstract "Antibody arrays are a useful for detecting antigens and other antibodies. This technique typically requires a uniform and well-defined orientation of antibodies attached to a surface for optimal performance. A uniform orientation can be achieved by modification of antibodies to include a single site for attachment. Thus, uniformly oriented antibody arrays require a bioengineered modification for the antibodies directly immobilization on the solid surface. In this study, we describe a “sandwich-type” antibody array where unmodified antibodies are oriented through binding with regioselectively immobilized recombinant antibody-binding protein L. Recombinant proL-CVIA bearing C-terminal CVIA motif is post-translationally modified with an alkyne group by protein farnesyltransferase (PFTase) at the cysteine residue in the CVIA sequence to give proL-CVIApf, which is covalently attached to an azido-modified glass slide by a Huisgen [3 + 2] cycloaddition reaction. Slides bearing antibodies bound to slides coated with regioselectively immobilized proL-CVIApf gave stronger fluorescence outputs and those where the antibody-binding protein was immobilized in random orientations on an epoxy-modified slide. Properly selected capture and detection antibodies did not cross-react with immobilized proL-CVIApf in sandwich arrays, and the proL-CVIApf slides can be used for multiple cycles of detected over a period of several months." @default.
• W2004452311 created "2016-06-24" @default.
• W2004452311 creator A5011579919 @default.
• W2004452311 creator A5036710196 @default.
• W2004452311 date "2014-05-30" @default.
• W2004452311 modified "2023-09-29" @default.
• W2004452311 title "Sandwich Antibody Arrays Using Recombinant Antibody-Binding Protein L" @default.
• W2004452311 cites W1496699014 @default.
• W2004452311 cites W1544101325 @default.
• W2004452311 cites W1554288134 @default.
• W2004452311 cites W1972718666 @default.
• W2004452311 cites W1991966183 @default.
• W2004452311 cites W1993418815 @default.
• W2004452311 cites W1994179443 @default.
• W2004452311 cites W2002768661 @default.
• W2004452311 cites W2003071799 @default.
• W2004452311 cites W2010305352 @default.
• W2004452311 cites W2019741068 @default.
• W2004452311 cites W2023433298 @default.
• W2004452311 cites W2025692302 @default.
• W2004452311 cites W2032736963 @default.
• W2004452311 cites W2032910143 @default.
• W2004452311 cites W2035699283 @default.
• W2004452311 cites W2037217106 @default.
• W2004452311 cites W2039719273 @default.
• W2004452311 cites W2042650834 @default.
• W2004452311 cites W2045354562 @default.
• W2004452311 cites W2056034367 @default.
• W2004452311 cites W2056539144 @default.
• W2004452311 cites W2058095761 @default.
• W2004452311 cites W2061216289 @default.
• W2004452311 cites W2065304415 @default.
• W2004452311 cites W2069618571 @default.
• W2004452311 cites W2075150637 @default.
• W2004452311 cites W2075880928 @default.
• W2004452311 cites W2084855813 @default.
• W2004452311 cites W2097086637 @default.
• W2004452311 cites W2113271180 @default.
• W2004452311 cites W2116988397 @default.
• W2004452311 cites W2130255165 @default.
• W2004452311 cites W2130832525 @default.
• W2004452311 cites W2144423928 @default.
• W2004452311 cites W2144991441 @default.
• W2004452311 cites W2145662716 @default.
• W2004452311 cites W2146218061 @default.
• W2004452311 cites W2322807693 @default.
• W2004452311 cites W2464484593 @default.
• W2004452311 doi "https://doi.org/10.1021/la500822w" @default.
• W2004452311 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4059220" @default.
• W2004452311 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24841983" @default.
• W2004452311 hasPublicationYear "2014" @default.
• W2004452311 type Work @default.
• W2004452311 sameAs 2004452311 @default.
• W2004452311 citedByCount "7" @default.
• W2004452311 countsByYear W20044523112014 @default.
• W2004452311 countsByYear W20044523112018 @default.
• W2004452311 countsByYear W20044523112019 @default.
• W2004452311 countsByYear W20044523112021 @default.
• W2004452311 countsByYear W20044523112022 @default.
• W2004452311 crossrefType "journal-article" @default.
• W2004452311 hasAuthorship W2004452311A5011579919 @default.
• W2004452311 hasAuthorship W2004452311A5036710196 @default.
• W2004452311 hasBestOaLocation W20044523111 @default.
• W2004452311 hasConcept C104317684 @default.
• W2004452311 hasConcept C147483822 @default.
• W2004452311 hasConcept C147816474 @default.
• W2004452311 hasConcept C153911025 @default.
• W2004452311 hasConcept C159654299 @default.
• W2004452311 hasConcept C178790620 @default.
• W2004452311 hasConcept C180577832 @default.
• W2004452311 hasConcept C181199279 @default.
• W2004452311 hasConcept C185592680 @default.
• W2004452311 hasConcept C203014093 @default.
• W2004452311 hasConcept C2778396349 @default.
• W2004452311 hasConcept C40767141 @default.
• W2004452311 hasConcept C54355233 @default.
• W2004452311 hasConcept C55493867 @default.
• W2004452311 hasConcept C57790582 @default.
• W2004452311 hasConcept C86803240 @default.
• W2004452311 hasConceptScore W2004452311C104317684 @default.
• W2004452311 hasConceptScore W2004452311C147483822 @default.
• W2004452311 hasConceptScore W2004452311C147816474 @default.
• W2004452311 hasConceptScore W2004452311C153911025 @default.
• W2004452311 hasConceptScore W2004452311C159654299 @default.
• W2004452311 hasConceptScore W2004452311C178790620 @default.
• W2004452311 hasConceptScore W2004452311C180577832 @default.
• W2004452311 hasConceptScore W2004452311C181199279 @default.
• W2004452311 hasConceptScore W2004452311C185592680 @default.
• W2004452311 hasConceptScore W2004452311C203014093 @default.
• W2004452311 hasConceptScore W2004452311C2778396349 @default.
• W2004452311 hasConceptScore W2004452311C40767141 @default.
• W2004452311 hasConceptScore W2004452311C54355233 @default.
• W2004452311 hasConceptScore W2004452311C55493867 @default.
• W2004452311 hasConceptScore W2004452311C57790582 @default.
• W2004452311 hasConceptScore W2004452311C86803240 @default.
• W2004452311 hasFunder F4320332161 @default.
• W2004452311 hasIssue "22" @default.
• W2004452311 hasLocation W20044523111 @default.

• next