Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004455217> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2004455217 abstract "We have described that insulin induces both NADPH oxidase-dependent ROS production and Ca2+ increase in skeletal muscle cells and although insulin-regulated GLUT4 traffic has been widely explored, the role of intracellular Ca2+ handling in this process is poorly understood. We studied insulin-induced GLUT4 traffic in GLUT4myc permanently transfected L6 skeletal muscle cells.Insulin-induced exofacial exposure of myc epitope, glucose uptake and Akt activation were independent of extracellular Ca2+ levels. Insulin increased H2O2 production measured with the cytosolic molecular sensor HyPer which was inhibited by NAC, a potent antioxidant. p47 subunit of NADPHox labeled only differentiated myotubes. Antioxidant agents, NAC and trolox partly reduced the externalization of myc epitope as did a specific inhibitor of NADPHox and overexpression of catalase. Superoxide dismutase 1 appears to potentiate the insulin effect. H2O2 induced Ca2+ release, which was inhibited by pre-incubation with ryanodine. Cytosol directed parvalbumin-DsRed reduced insulin-dependent exofacial exposure of myc epitope. Moreover, both ryanodine and xestospongin B partly inhibited myc exposure with an additive effect. Insulin induced an increase in the S-glutathionylation state of RyR1, detected by proximity ligation assay. Insulin induced an increase in mitochondrial ROS and Ca2+ levels measured with ratiometric HyPer and PeriCam respectivelly. The mitochondrial potential and oxygen consumption were also increased by insulin and inhibited by pre-incubation with IP3R blockers. Insulin-dependent GLUT4myc translocation to cell surface was strongly reduced in presence of ruthenium red. These data suggest that insulin induces an increase in cytoplasmic Ca2+ levels by NADPH-dependent RyR1 modifications and IP3R-dependent mitochondrial regulation in skeletal muscle myotubes.This work was supported by FONDECYT 3110170, FONDECYT 3110105, FONDECYT 1080436, ACT1111." @default.
- W2004455217 created "2016-06-24" @default.
- W2004455217 creator A5010563191 @default.
- W2004455217 creator A5018462821 @default.
- W2004455217 creator A5022277890 @default.
- W2004455217 creator A5031748181 @default.
- W2004455217 creator A5040659996 @default.
- W2004455217 creator A5060525055 @default.
- W2004455217 creator A5064467789 @default.
- W2004455217 creator A5087808358 @default.
- W2004455217 date "2013-01-01" @default.
- W2004455217 modified "2023-10-08" @default.
- W2004455217 title "Insulin Induces both H2O2 Production and IP3-Dependent Mitochondria Ca2+ Uptake. H2O2 Oxidizes RyR to Elicit Ca2+ Release and GLUT4 Translocation in Skeletal Muscle Cells" @default.
- W2004455217 doi "https://doi.org/10.1016/j.bpj.2012.11.3414" @default.
- W2004455217 hasPublicationYear "2013" @default.
- W2004455217 type Work @default.
- W2004455217 sameAs 2004455217 @default.
- W2004455217 citedByCount "0" @default.
- W2004455217 crossrefType "journal-article" @default.
- W2004455217 hasAuthorship W2004455217A5010563191 @default.
- W2004455217 hasAuthorship W2004455217A5018462821 @default.
- W2004455217 hasAuthorship W2004455217A5022277890 @default.
- W2004455217 hasAuthorship W2004455217A5031748181 @default.
- W2004455217 hasAuthorship W2004455217A5040659996 @default.
- W2004455217 hasAuthorship W2004455217A5060525055 @default.
- W2004455217 hasAuthorship W2004455217A5064467789 @default.
- W2004455217 hasAuthorship W2004455217A5087808358 @default.
- W2004455217 hasBestOaLocation W20044552171 @default.
- W2004455217 hasConcept C113217602 @default.
- W2004455217 hasConcept C126322002 @default.
- W2004455217 hasConcept C134018914 @default.
- W2004455217 hasConcept C161573976 @default.
- W2004455217 hasConcept C185592680 @default.
- W2004455217 hasConcept C2776188179 @default.
- W2004455217 hasConcept C2777866211 @default.
- W2004455217 hasConcept C2779306644 @default.
- W2004455217 hasConcept C2779959927 @default.
- W2004455217 hasConcept C55493867 @default.
- W2004455217 hasConcept C71924100 @default.
- W2004455217 hasConcept C79879829 @default.
- W2004455217 hasConcept C86803240 @default.
- W2004455217 hasConceptScore W2004455217C113217602 @default.
- W2004455217 hasConceptScore W2004455217C126322002 @default.
- W2004455217 hasConceptScore W2004455217C134018914 @default.
- W2004455217 hasConceptScore W2004455217C161573976 @default.
- W2004455217 hasConceptScore W2004455217C185592680 @default.
- W2004455217 hasConceptScore W2004455217C2776188179 @default.
- W2004455217 hasConceptScore W2004455217C2777866211 @default.
- W2004455217 hasConceptScore W2004455217C2779306644 @default.
- W2004455217 hasConceptScore W2004455217C2779959927 @default.
- W2004455217 hasConceptScore W2004455217C55493867 @default.
- W2004455217 hasConceptScore W2004455217C71924100 @default.
- W2004455217 hasConceptScore W2004455217C79879829 @default.
- W2004455217 hasConceptScore W2004455217C86803240 @default.
- W2004455217 hasLocation W20044552171 @default.
- W2004455217 hasOpenAccess W2004455217 @default.
- W2004455217 hasPrimaryLocation W20044552171 @default.
- W2004455217 hasRelatedWork W1528650954 @default.
- W2004455217 hasRelatedWork W166370444 @default.
- W2004455217 hasRelatedWork W1984928606 @default.
- W2004455217 hasRelatedWork W1985088193 @default.
- W2004455217 hasRelatedWork W1987613834 @default.
- W2004455217 hasRelatedWork W1995893209 @default.
- W2004455217 hasRelatedWork W2001789499 @default.
- W2004455217 hasRelatedWork W2008646227 @default.
- W2004455217 hasRelatedWork W2052016639 @default.
- W2004455217 hasRelatedWork W2052167458 @default.
- W2004455217 hasRelatedWork W2074122313 @default.
- W2004455217 hasRelatedWork W2094338116 @default.
- W2004455217 hasRelatedWork W2107615542 @default.
- W2004455217 hasRelatedWork W2118449713 @default.
- W2004455217 hasRelatedWork W2122184315 @default.
- W2004455217 hasRelatedWork W2123440524 @default.
- W2004455217 hasRelatedWork W2150714767 @default.
- W2004455217 hasRelatedWork W2754346643 @default.
- W2004455217 hasRelatedWork W2795758958 @default.
- W2004455217 hasRelatedWork W68513861 @default.
- W2004455217 isParatext "false" @default.
- W2004455217 isRetracted "false" @default.
- W2004455217 magId "2004455217" @default.
- W2004455217 workType "article" @default.