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• W2004510050 abstract "RationaleInduction of allergen specific IgG antibodies with capacity to block allergen-IgE interaction has been suggested as mechanism for clinical efficacy, and long term benefit of allergen specific immunotherapy. Induction of these blocking antibodies by depigmented-polymerized allergoids (Dpg-Pol) has been demonstrated previously. The objectives of this study were to investigate the epitope specificity of IgG antibodies induced by Dpg-Pol and native unmodified allergen extracts of birch pollen (Betula alba).MethodsSera from rabbits immunized with native and Dpg-Pol extracts of birch pollen were obtained, and specific IgG to birch, Bet v 1 and Bet t v 2 were measured by ELISA. Linear synthetic peptides of 12 aa, overlapping 6 aa, of Bet v 1 and Bet v 2 were covalently bound to a cellulose membrane, incubated with rabbit sera and with the secondary antibody, and developed by chemiluminiscence.ResultsSerum samples from rabbits immunized with native birch pollen extracts recognised 11 epitopes from Bet v 1, while serum samples from Dpg-Pol-immunized animals recognised 8. For Bet v 2, 8 epitopes were recognized by IgG from animals immunized with native extracts, and 9 epitopes from Dpg-Pol immunized animals. There are some epitopes recognized by both extracts, but some of them are recognized only by the Dpg-Pol extract.ConclusionsDepigmented-polymerized birch pollen allergen extract stimulates the synthesis of specific IgG antibodies which recognize common but also novel epitopes from the major allergens Bet v 1 and Bet v 2, which may be part of the mechanism of action of this treatment when used for allergen immunotherapy. RationaleInduction of allergen specific IgG antibodies with capacity to block allergen-IgE interaction has been suggested as mechanism for clinical efficacy, and long term benefit of allergen specific immunotherapy. Induction of these blocking antibodies by depigmented-polymerized allergoids (Dpg-Pol) has been demonstrated previously. The objectives of this study were to investigate the epitope specificity of IgG antibodies induced by Dpg-Pol and native unmodified allergen extracts of birch pollen (Betula alba). Induction of allergen specific IgG antibodies with capacity to block allergen-IgE interaction has been suggested as mechanism for clinical efficacy, and long term benefit of allergen specific immunotherapy. Induction of these blocking antibodies by depigmented-polymerized allergoids (Dpg-Pol) has been demonstrated previously. The objectives of this study were to investigate the epitope specificity of IgG antibodies induced by Dpg-Pol and native unmodified allergen extracts of birch pollen (Betula alba). MethodsSera from rabbits immunized with native and Dpg-Pol extracts of birch pollen were obtained, and specific IgG to birch, Bet v 1 and Bet t v 2 were measured by ELISA. Linear synthetic peptides of 12 aa, overlapping 6 aa, of Bet v 1 and Bet v 2 were covalently bound to a cellulose membrane, incubated with rabbit sera and with the secondary antibody, and developed by chemiluminiscence. Sera from rabbits immunized with native and Dpg-Pol extracts of birch pollen were obtained, and specific IgG to birch, Bet v 1 and Bet t v 2 were measured by ELISA. Linear synthetic peptides of 12 aa, overlapping 6 aa, of Bet v 1 and Bet v 2 were covalently bound to a cellulose membrane, incubated with rabbit sera and with the secondary antibody, and developed by chemiluminiscence. ResultsSerum samples from rabbits immunized with native birch pollen extracts recognised 11 epitopes from Bet v 1, while serum samples from Dpg-Pol-immunized animals recognised 8. For Bet v 2, 8 epitopes were recognized by IgG from animals immunized with native extracts, and 9 epitopes from Dpg-Pol immunized animals. There are some epitopes recognized by both extracts, but some of them are recognized only by the Dpg-Pol extract. Serum samples from rabbits immunized with native birch pollen extracts recognised 11 epitopes from Bet v 1, while serum samples from Dpg-Pol-immunized animals recognised 8. For Bet v 2, 8 epitopes were recognized by IgG from animals immunized with native extracts, and 9 epitopes from Dpg-Pol immunized animals. There are some epitopes recognized by both extracts, but some of them are recognized only by the Dpg-Pol extract. ConclusionsDepigmented-polymerized birch pollen allergen extract stimulates the synthesis of specific IgG antibodies which recognize common but also novel epitopes from the major allergens Bet v 1 and Bet v 2, which may be part of the mechanism of action of this treatment when used for allergen immunotherapy. Depigmented-polymerized birch pollen allergen extract stimulates the synthesis of specific IgG antibodies which recognize common but also novel epitopes from the major allergens Bet v 1 and Bet v 2, which may be part of the mechanism of action of this treatment when used for allergen immunotherapy." @default.
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• W2004510050 date "2013-02-01" @default.
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• W2004510050 title "Depigmented-Polymerized and Native Extracts of Birch Pollen Exhibit Different IgG Epitopes Specific to Bet v 1 and Bet v 2" @default.
• W2004510050 doi "https://doi.org/10.1016/j.jaci.2012.12.1064" @default.
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