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- W2004567572 abstract "Nitrous acid-induced ad-3B mutants of Neurospora crassa were tested for revertibility after treatment with hydroxylamine (HA). Mutants which reverted after the treatment were classified as having guanine-cytosine (GC) at the mutant site. A high frequency (86.5%) of nitrous acid-induced ad-3B mutants resulting from base-pair transitions have GC at the mutant site, whereas in bacteriophage T4 most nitrous acid-induced rII mutants have adenine-thymine (AT) at the mutant site. This apparent contradiction is discussed. In a random sampling of base-pair transition ad-3B mutants, we found among mutants with nonpolarized complementation pattern a lower incidence (2.3%) of AT at the mutant site than among mutants with polarized complementation pattern (25.0%) and among noncomplementing mutants (55.0%). Nonsense mutants, which specify the RNA triplets UAA, UAG, or UGA, and presumably the DNA anticodons ATT, ATC or ACT, should not be able to revert by a GC → AT transition, because by reaction with hydroxylamine the two C-containing triplets would be transformed to ATT, a nonsense triplet. The difference in the frequency of the GC pairs at mutant sites among the three different classes of complementation response indicates that nonsense mutations occur more frequently among mutants with polarized complementation patterns and among noncomplementing mutants than among mutants with nonpolarized complementation patterns." @default.
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- W2004567572 title "Correlation between base-pair transition and complementation pattern in nitrous acid-induced ad-3B mutants of neurospora crassa" @default.
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- W2004567572 doi "https://doi.org/10.1016/0027-5107(68)90006-7" @default.
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