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• W2004574865 endingPage "e113158" @default.
• W2004574865 startingPage "e113158" @default.
• W2004574865 abstract "Oxidative stress is an important mechanism of chemical toxicity, contributing to teratogenesis and to cardiovascular and neurodegenerative diseases. Developing animals may be especially sensitive to chemicals causing oxidative stress. The developmental expression and inducibility of anti-oxidant defenses through activation of NF-E2-related factor 2 (NRF2) affect susceptibility to oxidants, but the embryonic response to oxidants is not well understood. To assess the response to chemically mediated oxidative stress and how it may vary during development, zebrafish embryos, eleutheroembryos, or larvae at 1, 2, 3, 4, 5, and 6 days post fertilization (dpf) were exposed to DMSO (0.1%), tert-butylhydroquinone (tBHQ; 10 µM) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; 2 nM) for 6 hr. Transcript abundance was assessed by real-time qRT-PCR and microarray. qRT-PCR showed strong (4- to 5-fold) induction of gstp1 by tBHQ as early as 1 dpf. tBHQ also induced gclc (2 dpf), but not sod1, nqo1, or cyp1a. TCDD induced cyp1a but none of the other genes. Microarray analysis showed that 1477 probes were significantly different among the DMSO-, tBHQ-, and TCDD-treated eleutheroembryos at 4 dpf. There was substantial overlap between genes induced in developing zebrafish and a set of marker genes induced by oxidative stress in mammals. Genes induced by tBHQ in 4-dpf zebrafish included those involved in glutathione synthesis and utilization, signal transduction, and DNA damage/stress response. The strong induction of hsp70 determined by microarray was confirmed by qRT-PCR and by use of transgenic zebrafish expressing enhanced green fluorescent protein (EGFP) under control of the hsp70 promoter. Genes strongly down-regulated by tBHQ included mitfa, providing a molecular explanation for the loss of pigmentation in tBHQ-exposed embryos. These data show that zebrafish embryos are responsive to oxidative stress as early as 1 dpf, that responsiveness varies with development in a gene-specific manner, and that the oxidative stress response is substantially conserved in vertebrate animals." @default.
• W2004574865 created "2016-06-24" @default.
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• W2004574865 date "2014-11-17" @default.
• W2004574865 modified "2023-09-26" @default.
• W2004574865 title "The Transcriptional Response to Oxidative Stress during Vertebrate Development: Effects of tert-Butylhydroquinone and 2,3,7,8-Tetrachlorodibenzo-p-Dioxin" @default.
• W2004574865 cites W1516095885 @default.
• W2004574865 cites W1533811499 @default.
• W2004574865 cites W1643210644 @default.
• W2004574865 cites W182547225 @default.
• W2004574865 cites W1962982449 @default.
• W2004574865 cites W1963796435 @default.
• W2004574865 cites W1964895626 @default.
• W2004574865 cites W1966006200 @default.
• W2004574865 cites W1966299493 @default.
• W2004574865 cites W1966952812 @default.
• W2004574865 cites W1967640859 @default.
• W2004574865 cites W1969712562 @default.
• W2004574865 cites W1972762617 @default.
• W2004574865 cites W1974427398 @default.
• W2004574865 cites W1976709591 @default.
• W2004574865 cites W1977426928 @default.
• W2004574865 cites W1979209667 @default.
• W2004574865 cites W1981239255 @default.
• W2004574865 cites W1982635732 @default.
• W2004574865 cites W1985376182 @default.
• W2004574865 cites W1987122345 @default.
• W2004574865 cites W1987868031 @default.
• W2004574865 cites W1993050097 @default.
• W2004574865 cites W1993250284 @default.
• W2004574865 cites W1998276272 @default.
• W2004574865 cites W1998756807 @default.
• W2004574865 cites W1998863653 @default.
• W2004574865 cites W2005464539 @default.
• W2004574865 cites W2006398724 @default.
• W2004574865 cites W2008208329 @default.
• W2004574865 cites W2008308093 @default.
• W2004574865 cites W2011762251 @default.
• W2004574865 cites W2013292686 @default.
• W2004574865 cites W2013612914 @default.
• W2004574865 cites W2015392958 @default.
• W2004574865 cites W2015647734 @default.
• W2004574865 cites W2018027837 @default.
• W2004574865 cites W2018888672 @default.
• W2004574865 cites W2027979403 @default.
• W2004574865 cites W2030061880 @default.
• W2004574865 cites W2031748375 @default.
• W2004574865 cites W2031900188 @default.
• W2004574865 cites W2034224169 @default.
• W2004574865 cites W2034741926 @default.
• W2004574865 cites W2035338698 @default.
• W2004574865 cites W2036297991 @default.
• W2004574865 cites W2037880480 @default.
• W2004574865 cites W2038576120 @default.
• W2004574865 cites W2039242242 @default.
• W2004574865 cites W2039688487 @default.
• W2004574865 cites W2040281845 @default.
• W2004574865 cites W2044087381 @default.
• W2004574865 cites W2045787491 @default.
• W2004574865 cites W2046020232 @default.
• W2004574865 cites W2048638521 @default.
• W2004574865 cites W2053818267 @default.
• W2004574865 cites W2055238787 @default.
• W2004574865 cites W2055809088 @default.
• W2004574865 cites W2059702269 @default.
• W2004574865 cites W2060646272 @default.
• W2004574865 cites W2064197222 @default.
• W2004574865 cites W2065540551 @default.
• W2004574865 cites W2067473651 @default.
• W2004574865 cites W2067490578 @default.
• W2004574865 cites W2068884628 @default.
• W2004574865 cites W2069094807 @default.
• W2004574865 cites W2069159655 @default.
• W2004574865 cites W2071137645 @default.
• W2004574865 cites W2075757290 @default.
• W2004574865 cites W2077984495 @default.
• W2004574865 cites W2079239233 @default.
• W2004574865 cites W2084200591 @default.
• W2004574865 cites W2084310233 @default.
• W2004574865 cites W2087389434 @default.
• W2004574865 cites W2088814515 @default.
• W2004574865 cites W2093654293 @default.
• W2004574865 cites W2093695182 @default.
• W2004574865 cites W2093752294 @default.
• W2004574865 cites W2095466273 @default.
• W2004574865 cites W2097506454 @default.
• W2004574865 cites W2099589230 @default.
• W2004574865 cites W2103164463 @default.
• W2004574865 cites W2103854462 @default.

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