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- W2004584962 abstract "Model of study: Experimental study. Introduction: Recently, stem cell research has generated greatinterest due to its applicability in regenerative medicine. Bone marrow is considered the most importantsource of adult stem cells and the establishment of new methods towards gene expression analysisregarding stem cells has become necessary. Thus Differential Display Reverse Transcription Polymerase Chain Reaction (DDRT-PCR) may be an accessible tool to investigate small differences in the geneexpression of different stem cells in distinct situations.Aim: In the present study, we investigated the exequibility of DDRT-PCR to identify differences in globalgene expression of mice bone marrow cells under two conditions.Methods: First, bone marrow cells were isolated fresh and a part was cultivated during one week withoutmedium replacement. Afterwards, both bone marrow cells (fresh and cultivated) were submitted to geneexpression analyses by DDRT-PCR.Results: Initially, it was possible to observe in one week-cultured bone marrow cells, changes in morphology (oval cells to fibroblastic-like cells) and protein profile, which was seen through differences inband distribution in SDS-Page gels. Finally through gene expression analysis, we detected three bands(1300, 1000 and 225 bp) exclusively expressed in the fresh bone marrow group and two bands (400 and300 bp) expressed specifically in the cultivated bone marrow cell group.Conclusions: In summary, the DDRT-PCR method was proved efficient towards the identification ofsmall differences in gene expression of bone marrow cells in two defined conditions. Thus, we expectthat DDRT-PCR can be fast and efficiently designed to analyze differential gene expression in severalstem cell types under distinct conditions." @default.
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- W2004584962 date "2012-12-30" @default.
- W2004584962 modified "2023-10-16" @default.
- W2004584962 title "Exequibilidade do DDRT-PCR para análise da expressão gênica diferencial em células de medula óssea murina" @default.
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- W2004584962 doi "https://doi.org/10.11606/issn.2176-7262.v45i4p428-435" @default.
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