FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004642364> ?p ?o ?g. }

• W2004642364 endingPage "211" @default.
• W2004642364 startingPage "203" @default.
• W2004642364 abstract "Endo-(1,4)-beta-xylanases of plant and fungal origin play an important role in the degradation of arabinoxylans. Two distinct classes of proteinaceous endoxylanase inhibitors, the Triticum aestivum xylanase inhibitor (TAXI) and the xylanase inhibitor protein (XIP), have been identified in cereals. Engineering of proteins in conjunction with enzyme kinetics, thermodynamic, real-time interaction, and X-ray crystallographic studies has provided knowledge on the mechanism of inhibition of XIP-I towards endoxylanases. XIP-I is a 30 kDa protein which belongs to glycoside hydrolase family 18, and folds as a typical (beta/alpha)8 barrel. Although the inhibitor shows highest homology with plant chitinases, XIP-I does not hydrolyse chitin; probably due to structural differences in the XIP-I binding cleft. The inhibitor is specific for fungal xylanases from glycoside hydrolases families 10 and 11, but does not inhibit bacterial enzymes. The inhibition is competitive and, depending on the xylanase, the Ki value can be as low as 3.4 nM. Site-directed mutagenesis of a xylanase from Aspergillus niger suggested that the XIP-I binding site was the conserved hairpin loop thumb region of family 11 xylanases. Furthermore, XIP-I shows the ability to inhibit barley alpha-amylases of glycoside hydrolase family 13, providing the first example of a protein able to inhibit members of different glycoside hydrolase families (10, 11, and 13), and additionally a novel function for a protein of glycoside hydrolase family 18." @default.
• W2004642364 created "2016-06-24" @default.
• W2004642364 creator A5020427938 @default.
• W2004642364 creator A5026456663 @default.
• W2004642364 creator A5060590414 @default.
• W2004642364 date "2004-02-01" @default.
• W2004642364 modified "2023-10-05" @default.
• W2004642364 title "XIP-I, a xylanase inhibitor protein from wheat: a novel protein function" @default.
• W2004642364 cites W1497665647 @default.
• W2004642364 cites W154700294 @default.
• W2004642364 cites W1577400047 @default.
• W2004642364 cites W1607829049 @default.
• W2004642364 cites W1841761311 @default.
• W2004642364 cites W1935712031 @default.
• W2004642364 cites W1968000602 @default.
• W2004642364 cites W1973606909 @default.
• W2004642364 cites W1979966636 @default.
• W2004642364 cites W1983165243 @default.
• W2004642364 cites W1989145266 @default.
• W2004642364 cites W1990098745 @default.
• W2004642364 cites W1990513516 @default.
• W2004642364 cites W1994398297 @default.
• W2004642364 cites W1998928910 @default.
• W2004642364 cites W1999174545 @default.
• W2004642364 cites W2001081709 @default.
• W2004642364 cites W2004073572 @default.
• W2004642364 cites W2004507339 @default.
• W2004642364 cites W2004548125 @default.
• W2004642364 cites W2008871343 @default.
• W2004642364 cites W2013900027 @default.
• W2004642364 cites W2018057877 @default.
• W2004642364 cites W2020253557 @default.
• W2004642364 cites W2024076899 @default.
• W2004642364 cites W2025591974 @default.
• W2004642364 cites W2028074250 @default.
• W2004642364 cites W2029238809 @default.
• W2004642364 cites W2030133436 @default.
• W2004642364 cites W2031592614 @default.
• W2004642364 cites W2035650331 @default.
• W2004642364 cites W2036722129 @default.
• W2004642364 cites W2041957907 @default.
• W2004642364 cites W2041964108 @default.
• W2004642364 cites W2041969301 @default.
• W2004642364 cites W2045801831 @default.
• W2004642364 cites W2046319395 @default.
• W2004642364 cites W2048537871 @default.
• W2004642364 cites W2050899554 @default.
• W2004642364 cites W2057386170 @default.
• W2004642364 cites W2060100899 @default.
• W2004642364 cites W2066944813 @default.
• W2004642364 cites W2068873912 @default.
• W2004642364 cites W2070566765 @default.
• W2004642364 cites W2071532027 @default.
• W2004642364 cites W2071945275 @default.
• W2004642364 cites W2072144575 @default.
• W2004642364 cites W2075770210 @default.
• W2004642364 cites W2084858774 @default.
• W2004642364 cites W2089723680 @default.
• W2004642364 cites W2096140760 @default.
• W2004642364 cites W2107368146 @default.
• W2004642364 cites W2108671897 @default.
• W2004642364 cites W2110722566 @default.
• W2004642364 cites W2112185869 @default.
• W2004642364 cites W2138049038 @default.
• W2004642364 cites W2147799946 @default.
• W2004642364 cites W2152208548 @default.
• W2004642364 cites W2168675755 @default.
• W2004642364 cites W2232517365 @default.
• W2004642364 cites W2266642515 @default.
• W2004642364 cites W238031030 @default.
• W2004642364 cites W4237920569 @default.
• W2004642364 cites W4240247729 @default.
• W2004642364 doi "https://doi.org/10.1016/j.bbapap.2003.08.014" @default.
• W2004642364 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14871661" @default.
• W2004642364 hasPublicationYear "2004" @default.
• W2004642364 type Work @default.
• W2004642364 sameAs 2004642364 @default.
• W2004642364 citedByCount "99" @default.
• W2004642364 countsByYear W20046423642012 @default.
• W2004642364 countsByYear W20046423642013 @default.
• W2004642364 countsByYear W20046423642014 @default.
• W2004642364 countsByYear W20046423642015 @default.
• W2004642364 countsByYear W20046423642016 @default.
• W2004642364 countsByYear W20046423642017 @default.
• W2004642364 countsByYear W20046423642018 @default.
• W2004642364 countsByYear W20046423642019 @default.
• W2004642364 countsByYear W20046423642020 @default.
• W2004642364 countsByYear W20046423642021 @default.
• W2004642364 countsByYear W20046423642023 @default.
• W2004642364 crossrefType "journal-article" @default.
• W2004642364 hasAuthorship W2004642364A5020427938 @default.
• W2004642364 hasAuthorship W2004642364A5026456663 @default.
• W2004642364 hasAuthorship W2004642364A5060590414 @default.
• W2004642364 hasConcept C104317684 @default.
• W2004642364 hasConcept C171897839 @default.
• W2004642364 hasConcept C181199279 @default.
• W2004642364 hasConcept C196032511 @default.
• W2004642364 hasConcept C2775859485 @default.

• next