Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004643787> ?p ?o ?g. }
- W2004643787 endingPage "158" @default.
- W2004643787 startingPage "151" @default.
- W2004643787 abstract "The isolation and multiple genotyping of long individual DNA fragments are needed to obtain haplotype information for diploid organisms. Limiting dilution of sample DNA followed by multiple displacement amplification is a useful technique but is restricted to short (<5 kb) DNA fragments. In the current study, a novel modification was applied to overcome these problems. A limited amount of cellular DNA was carefully released from intact cells into a mildly heated alkaline agarose solution and mixed thoroughly. The solution was then gently aliquoted and allowed to solidify while maintaining the integrity of the diluted DNA. Exogenously provided Phi29 DNA polymerase was used to perform consistent genomic amplification with random hexameric oligonucleotides within the agarose gels. Simple heat melting of the gel allowed recovery of the amplified materials in a solution of the polymerase chain reaction (PCR)-ready form. The haplotypes of seven SNPs spanning 240 kb of the DNA surrounding the human ATM gene region on chromosome 11 were determined for 10 individuals, demonstrating the feasibility of this new method." @default.
- W2004643787 created "2016-06-24" @default.
- W2004643787 creator A5005806096 @default.
- W2004643787 creator A5009938658 @default.
- W2004643787 creator A5016927893 @default.
- W2004643787 creator A5029489298 @default.
- W2004643787 creator A5048652457 @default.
- W2004643787 creator A5056466207 @default.
- W2004643787 creator A5065575216 @default.
- W2004643787 creator A5068937766 @default.
- W2004643787 creator A5080549041 @default.
- W2004643787 creator A5091577963 @default.
- W2004643787 date "2008-12-01" @default.
- W2004643787 modified "2023-09-24" @default.
- W2004643787 title "In-gel multiple displacement amplification of long DNA fragments diluted to the single molecule level" @default.
- W2004643787 cites W1583084344 @default.
- W2004643787 cites W1750162611 @default.
- W2004643787 cites W175381353 @default.
- W2004643787 cites W1946936739 @default.
- W2004643787 cites W1968762427 @default.
- W2004643787 cites W1979261770 @default.
- W2004643787 cites W1981675400 @default.
- W2004643787 cites W1984731778 @default.
- W2004643787 cites W1990403938 @default.
- W2004643787 cites W1994108584 @default.
- W2004643787 cites W2006782785 @default.
- W2004643787 cites W2031244943 @default.
- W2004643787 cites W2037325598 @default.
- W2004643787 cites W2047612868 @default.
- W2004643787 cites W2050633988 @default.
- W2004643787 cites W2050778521 @default.
- W2004643787 cites W2069574509 @default.
- W2004643787 cites W2079641200 @default.
- W2004643787 cites W2080963534 @default.
- W2004643787 cites W2086814185 @default.
- W2004643787 cites W2093166014 @default.
- W2004643787 cites W2097331522 @default.
- W2004643787 cites W2097348296 @default.
- W2004643787 cites W2105306382 @default.
- W2004643787 cites W2107233609 @default.
- W2004643787 cites W2136886129 @default.
- W2004643787 cites W2145903120 @default.
- W2004643787 cites W2147575498 @default.
- W2004643787 cites W2155307866 @default.
- W2004643787 cites W2157455890 @default.
- W2004643787 cites W2165266236 @default.
- W2004643787 cites W2166061027 @default.
- W2004643787 cites W2217809488 @default.
- W2004643787 cites W2579829632 @default.
- W2004643787 cites W4300445024 @default.
- W2004643787 cites W2120579498 @default.
- W2004643787 doi "https://doi.org/10.1016/j.ab.2008.08.011" @default.
- W2004643787 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18768135" @default.
- W2004643787 hasPublicationYear "2008" @default.
- W2004643787 type Work @default.
- W2004643787 sameAs 2004643787 @default.
- W2004643787 citedByCount "7" @default.
- W2004643787 countsByYear W20046437872012 @default.
- W2004643787 countsByYear W20046437872020 @default.
- W2004643787 crossrefType "journal-article" @default.
- W2004643787 hasAuthorship W2004643787A5005806096 @default.
- W2004643787 hasAuthorship W2004643787A5009938658 @default.
- W2004643787 hasAuthorship W2004643787A5016927893 @default.
- W2004643787 hasAuthorship W2004643787A5029489298 @default.
- W2004643787 hasAuthorship W2004643787A5048652457 @default.
- W2004643787 hasAuthorship W2004643787A5056466207 @default.
- W2004643787 hasAuthorship W2004643787A5065575216 @default.
- W2004643787 hasAuthorship W2004643787A5068937766 @default.
- W2004643787 hasAuthorship W2004643787A5080549041 @default.
- W2004643787 hasAuthorship W2004643787A5091577963 @default.
- W2004643787 hasConcept C104317684 @default.
- W2004643787 hasConcept C112456955 @default.
- W2004643787 hasConcept C129312508 @default.
- W2004643787 hasConcept C135763542 @default.
- W2004643787 hasConcept C153911025 @default.
- W2004643787 hasConcept C156385826 @default.
- W2004643787 hasConcept C17757408 @default.
- W2004643787 hasConcept C185592680 @default.
- W2004643787 hasConcept C2756471 @default.
- W2004643787 hasConcept C2778476535 @default.
- W2004643787 hasConcept C31467283 @default.
- W2004643787 hasConcept C49105822 @default.
- W2004643787 hasConcept C52001828 @default.
- W2004643787 hasConcept C54355233 @default.
- W2004643787 hasConcept C552990157 @default.
- W2004643787 hasConcept C78063203 @default.
- W2004643787 hasConcept C82381507 @default.
- W2004643787 hasConcept C86803240 @default.
- W2004643787 hasConcept C90583042 @default.
- W2004643787 hasConceptScore W2004643787C104317684 @default.
- W2004643787 hasConceptScore W2004643787C112456955 @default.
- W2004643787 hasConceptScore W2004643787C129312508 @default.
- W2004643787 hasConceptScore W2004643787C135763542 @default.
- W2004643787 hasConceptScore W2004643787C153911025 @default.
- W2004643787 hasConceptScore W2004643787C156385826 @default.
- W2004643787 hasConceptScore W2004643787C17757408 @default.
- W2004643787 hasConceptScore W2004643787C185592680 @default.
- W2004643787 hasConceptScore W2004643787C2756471 @default.