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- W2004644255 abstract "The effects of the compositional alterations induced in the plasmalogen-rich phospholipids of Clostridium butyricum by growth at different temperatures with biotin or at a given temperature on supplementation with exogenous fatty acids in the absence of biotin, have been studied in lipid dispersions with the spin probe 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) and with the fluorescent probe diphenylhexatriene. Plots of TEMPO partitioning vs. temperature revealed deviations from linearity at 48° and 34.5°C for the phospholipids from cells grown on biotin at 37° and at 45° and 29° C for the phospholipids from cells grown on biotin at 25°C. Diphenylhexatriene fluorescence polarization showed a broad inflection beginning at 36°C for the lipids from 37°C grown cells and at 32°C for the lipids from 25°C-grown cells. Maximum polarization was reached at −5°C in each sample. Phospholipids from cells grown on oleate at 37°C had 85% 18 : 1 plus C19 cyclopropane acyl chains and 90% 18 : 1 plus C19-cyclopropane alk-1-enyl chains. The phospholipids had decreased proportions of ethanolamine plus N-methylethanolamine plasmalogens (Khuller, G.K. and Goldfine, H. (1975) Biochemistry 14, 3642) and markedly increased levels of the glycerol acetal derivatives of these plasmalogens. Deviations from linearity were seen in TEMPO partitioning plots at 44° and 28°C and diphenylhexatriene polarization showed a broad transition beginning at 29°C and ending at −2°C. Polarization did not, however, reach a maximum, even at −15°C." @default.
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- W2004644255 date "1977-09-01" @default.
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- W2004644255 title "Effects of growth temperature and supplementation with exogenous fatty acids on some physical properties of Clostridium butyricum phospholipids" @default.
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- W2004644255 doi "https://doi.org/10.1016/0005-2760(77)90193-x" @default.
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