Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004645295> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2004645295 abstract "This study explored the application of the alternative splicing of Bcl-2 family protein Mcl-1 in combination chemotherapy for head and neck squamous cell carcinoma (HNSCC) cell lines: meayamycin B (SF3b inhibitor) switched the pre-mRNA alternative splicing of Mcl-1 from anti-apoptotic to pro-apoptotic; the combination of meayamycin B with ABT-737 (Bcl-xL inhibitor) rapidly stimulated apoptosis in a panel of HNSCC cells, overcoming the HPV-16-mediated Mcl-1 over-expression. Western blotting and semi-quantitative RT-PCR as well as qPCR afforded evaluation of the expression of Mcl-1 splicing variants at mRNA and protein levels. Apoptosis status was monitored by using caspase 3/7 activity assays and Annexin V/7-AAD staining. siRNA was used to specifically knock down HPV-16 E6 gene. Meayamycin B exhibited 0.1-10 nM ED50 values in HNSCC cell lines that are mostly resistant to ABT-737. In HPV-16 positive HNSCC, E6 oncogene affords moderate resistance to meayamycin B. The combination of meayamycin B and ABT-737 synergistically induced apoptosis in HNSCC cell lines regardless of HPV status. These data demonstrate the potential of meayamycin B, both as a single agent and in combination with Bcl-xL inhibitors, for both research and targeted molecular therapeutic purposes for HNSCC. Citation Format: Yang Gao. SF3B1 inhibitors sensitize head and neck squamous cell carcinoma to Bcl-xL inhibition by regulating Mcl-1 alternative splicing. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2064. doi:10.1158/1538-7445.AM2013-2064" @default.
- W2004645295 created "2016-06-24" @default.
- W2004645295 creator A5022708781 @default.
- W2004645295 date "2013-04-15" @default.
- W2004645295 modified "2023-09-25" @default.
- W2004645295 title "Abstract 2064: SF3B1 inhibitors sensitize head and neck squamous cell carcinoma to Bcl-xL inhibition by regulating Mcl-1 alternative splicing." @default.
- W2004645295 doi "https://doi.org/10.1158/1538-7445.am2013-2064" @default.
- W2004645295 hasPublicationYear "2013" @default.
- W2004645295 type Work @default.
- W2004645295 sameAs 2004645295 @default.
- W2004645295 citedByCount "0" @default.
- W2004645295 crossrefType "proceedings-article" @default.
- W2004645295 hasAuthorship W2004645295A5022708781 @default.
- W2004645295 hasConcept C104317684 @default.
- W2004645295 hasConcept C105580179 @default.
- W2004645295 hasConcept C121608353 @default.
- W2004645295 hasConcept C126322002 @default.
- W2004645295 hasConcept C190283241 @default.
- W2004645295 hasConcept C194583182 @default.
- W2004645295 hasConcept C2776530083 @default.
- W2004645295 hasConcept C2776789625 @default.
- W2004645295 hasConcept C2776833033 @default.
- W2004645295 hasConcept C2781018059 @default.
- W2004645295 hasConcept C29537977 @default.
- W2004645295 hasConcept C31573885 @default.
- W2004645295 hasConcept C502942594 @default.
- W2004645295 hasConcept C54355233 @default.
- W2004645295 hasConcept C54458228 @default.
- W2004645295 hasConcept C67705224 @default.
- W2004645295 hasConcept C71924100 @default.
- W2004645295 hasConcept C86803240 @default.
- W2004645295 hasConcept C88634738 @default.
- W2004645295 hasConceptScore W2004645295C104317684 @default.
- W2004645295 hasConceptScore W2004645295C105580179 @default.
- W2004645295 hasConceptScore W2004645295C121608353 @default.
- W2004645295 hasConceptScore W2004645295C126322002 @default.
- W2004645295 hasConceptScore W2004645295C190283241 @default.
- W2004645295 hasConceptScore W2004645295C194583182 @default.
- W2004645295 hasConceptScore W2004645295C2776530083 @default.
- W2004645295 hasConceptScore W2004645295C2776789625 @default.
- W2004645295 hasConceptScore W2004645295C2776833033 @default.
- W2004645295 hasConceptScore W2004645295C2781018059 @default.
- W2004645295 hasConceptScore W2004645295C29537977 @default.
- W2004645295 hasConceptScore W2004645295C31573885 @default.
- W2004645295 hasConceptScore W2004645295C502942594 @default.
- W2004645295 hasConceptScore W2004645295C54355233 @default.
- W2004645295 hasConceptScore W2004645295C54458228 @default.
- W2004645295 hasConceptScore W2004645295C67705224 @default.
- W2004645295 hasConceptScore W2004645295C71924100 @default.
- W2004645295 hasConceptScore W2004645295C86803240 @default.
- W2004645295 hasConceptScore W2004645295C88634738 @default.
- W2004645295 hasLocation W20046452951 @default.
- W2004645295 hasOpenAccess W2004645295 @default.
- W2004645295 hasPrimaryLocation W20046452951 @default.
- W2004645295 hasRelatedWork W1417355860 @default.
- W2004645295 hasRelatedWork W1489498714 @default.
- W2004645295 hasRelatedWork W1559619181 @default.
- W2004645295 hasRelatedWork W1963776089 @default.
- W2004645295 hasRelatedWork W2028763328 @default.
- W2004645295 hasRelatedWork W2033954083 @default.
- W2004645295 hasRelatedWork W2135165501 @default.
- W2004645295 hasRelatedWork W2240367598 @default.
- W2004645295 hasRelatedWork W2337923700 @default.
- W2004645295 hasRelatedWork W2402591632 @default.
- W2004645295 hasRelatedWork W2505604536 @default.
- W2004645295 hasRelatedWork W2550174857 @default.
- W2004645295 hasRelatedWork W2575536631 @default.
- W2004645295 hasRelatedWork W2883155085 @default.
- W2004645295 hasRelatedWork W2955330936 @default.
- W2004645295 hasRelatedWork W3020377885 @default.
- W2004645295 hasRelatedWork W3037391733 @default.
- W2004645295 hasRelatedWork W3083200100 @default.
- W2004645295 hasRelatedWork W3117478370 @default.
- W2004645295 hasRelatedWork W3180766567 @default.
- W2004645295 isParatext "false" @default.
- W2004645295 isRetracted "false" @default.
- W2004645295 magId "2004645295" @default.
- W2004645295 workType "article" @default.