Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004656183> ?p ?o ?g. }
- W2004656183 endingPage "36" @default.
- W2004656183 startingPage "25" @default.
- W2004656183 abstract "Uteroferrin is an iron-binding glycoprotein, which is abundantly synthesized in porcine uterine glandular endometrium and believed to be involved in maternal/fetal iron transport. In the present study, uteroferrin has been cloned and functionally expressed using baculovirus-infected insect host cells Spodoptera frugiperda. The work also addresses the possible role of proteolytic cleavage to facilitate the release of uteroferrin-bound iron. The enzyme secreted in culture medium exhibits a molecular mass and catalytic properties similar to native porcine uteroferrin. The specific activity was estimated at 233 U/mg using p-nitrophenyl phosphate as substrate. Partial cleavage of the enzyme with trypsin resulted in a 1.7-fold enhancement in specific activity and a two-subunit polypeptide as observed in preparations of most mammalian purple acid phosphatases. Digestion with the aspartic protease pepsin resulted in a 2.5-fold enzyme inactivation correlated with the appearance of low molecular weight polypeptide fragments and the release of enzyme-bound iron." @default.
- W2004656183 created "2016-06-24" @default.
- W2004656183 creator A5023155028 @default.
- W2004656183 creator A5029562955 @default.
- W2004656183 creator A5030789591 @default.
- W2004656183 creator A5046175284 @default.
- W2004656183 creator A5051945237 @default.
- W2004656183 creator A5058185208 @default.
- W2004656183 creator A5078325680 @default.
- W2004656183 creator A5088297003 @default.
- W2004656183 date "2004-12-01" @default.
- W2004656183 modified "2023-09-23" @default.
- W2004656183 title "Porcine purple acid phosphatase: heterologous expression, characterization, and proteolytic analysis" @default.
- W2004656183 cites W135785445 @default.
- W2004656183 cites W1486803151 @default.
- W2004656183 cites W1490300323 @default.
- W2004656183 cites W1511804083 @default.
- W2004656183 cites W1515122089 @default.
- W2004656183 cites W1530428914 @default.
- W2004656183 cites W1545953723 @default.
- W2004656183 cites W1562906870 @default.
- W2004656183 cites W1571380461 @default.
- W2004656183 cites W1572506372 @default.
- W2004656183 cites W1572561340 @default.
- W2004656183 cites W1581754894 @default.
- W2004656183 cites W1582923620 @default.
- W2004656183 cites W1589594958 @default.
- W2004656183 cites W1590478241 @default.
- W2004656183 cites W1596570698 @default.
- W2004656183 cites W1605117581 @default.
- W2004656183 cites W1608282631 @default.
- W2004656183 cites W1822169786 @default.
- W2004656183 cites W1943108778 @default.
- W2004656183 cites W1966631093 @default.
- W2004656183 cites W1970569189 @default.
- W2004656183 cites W1975389760 @default.
- W2004656183 cites W1975499102 @default.
- W2004656183 cites W1984064440 @default.
- W2004656183 cites W1997304859 @default.
- W2004656183 cites W2013873050 @default.
- W2004656183 cites W2017626292 @default.
- W2004656183 cites W2018006157 @default.
- W2004656183 cites W2020280916 @default.
- W2004656183 cites W2020386376 @default.
- W2004656183 cites W2028700212 @default.
- W2004656183 cites W2034018882 @default.
- W2004656183 cites W2037471252 @default.
- W2004656183 cites W2040687623 @default.
- W2004656183 cites W2048781270 @default.
- W2004656183 cites W2073187113 @default.
- W2004656183 cites W2085153818 @default.
- W2004656183 cites W2099463774 @default.
- W2004656183 cites W2100837269 @default.
- W2004656183 cites W2109809969 @default.
- W2004656183 cites W2110842773 @default.
- W2004656183 cites W2112356012 @default.
- W2004656183 cites W2131628538 @default.
- W2004656183 cites W2165348927 @default.
- W2004656183 cites W2165539758 @default.
- W2004656183 cites W4293247451 @default.
- W2004656183 doi "https://doi.org/10.1016/j.abb.2004.08.008" @default.
- W2004656183 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15519293" @default.
- W2004656183 hasPublicationYear "2004" @default.
- W2004656183 type Work @default.
- W2004656183 sameAs 2004656183 @default.
- W2004656183 citedByCount "12" @default.
- W2004656183 countsByYear W20046561832013 @default.
- W2004656183 countsByYear W20046561832015 @default.
- W2004656183 countsByYear W20046561832018 @default.
- W2004656183 countsByYear W20046561832020 @default.
- W2004656183 countsByYear W20046561832021 @default.
- W2004656183 countsByYear W20046561832023 @default.
- W2004656183 crossrefType "journal-article" @default.
- W2004656183 hasAuthorship W2004656183A5023155028 @default.
- W2004656183 hasAuthorship W2004656183A5029562955 @default.
- W2004656183 hasAuthorship W2004656183A5030789591 @default.
- W2004656183 hasAuthorship W2004656183A5046175284 @default.
- W2004656183 hasAuthorship W2004656183A5051945237 @default.
- W2004656183 hasAuthorship W2004656183A5058185208 @default.
- W2004656183 hasAuthorship W2004656183A5078325680 @default.
- W2004656183 hasAuthorship W2004656183A5088297003 @default.
- W2004656183 hasConcept C104317684 @default.
- W2004656183 hasConcept C108625454 @default.
- W2004656183 hasConcept C151730666 @default.
- W2004656183 hasConcept C153911025 @default.
- W2004656183 hasConcept C175156509 @default.
- W2004656183 hasConcept C181199279 @default.
- W2004656183 hasConcept C182220744 @default.
- W2004656183 hasConcept C21547065 @default.
- W2004656183 hasConcept C2776714187 @default.
- W2004656183 hasConcept C2780115692 @default.
- W2004656183 hasConcept C2780340462 @default.
- W2004656183 hasConcept C41183919 @default.
- W2004656183 hasConcept C43369102 @default.
- W2004656183 hasConcept C55493867 @default.
- W2004656183 hasConcept C56856141 @default.
- W2004656183 hasConcept C86803240 @default.
- W2004656183 hasConceptScore W2004656183C104317684 @default.