Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004657461> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2004657461 endingPage "207" @default.
- W2004657461 startingPage "201" @default.
- W2004657461 abstract "Microcystins are potent hepatotoxins produced by cyanobacteria, and are also tumor promoters as well as potent inhibitors of the catalytic subunits of protein phosphatases 1 and 2A. In order to establish a physicochemical method for individual detection and determination of trace amounts of microcystins, we developed a derivatization method for fluorescence (FL) and chemiluminescence (CL) detection, in which a highly fluorescent dienophile, DMEQ-TAD (4-[2-(6,7-dimethoxy-4-methyl-3-oxo-3,4-dihydroquinoxalinyl) ethyl]-1,2,4-triazoline-3,5-dione), was used as the labeling reagent. DMEQ-TAD reacted smoothly with the conjugated diene of the Adda moiety to give 2 stereoisomers of the adducts. As a result of the extensive experiments, the following reaction conditions were optimized for the labeling: sample amount, 10 micrograms; reaction solvent, DMF:acetonitrile (1:1); reaction time, 15 minutes; reaction temperature, 70 degrees C; amount of DMEQ-TAD used relative to that of microcystin, 80 equivalent. The resulting 6 adducts from microcystins-LR, -YR, and -RR can be separated from one another using the following reversed phase HPLC conditions in combination with a clean-up using ODS silica gel: column, Cosmosil 5C18-AR (150 x 4.6 I.D. mm); mobile phase, methanol:0.05M phosphate buffer (pH 3) (1:1); flow rate, 1.0 ml/min; detection, FL lambda ex 370 nm, lambda em 440 nm. The detection limits of the DMEQ-TAD derivatives were estimated to be 100 and 500 pg for LR, and 65 and 2,500 pg for RR using FL and CL detections, respectively; and the detection behavior was different from that of the Dns-Cys derivatives, which were more sensitive to CL than FL." @default.
- W2004657461 created "2016-06-24" @default.
- W2004657461 creator A5003503677 @default.
- W2004657461 creator A5014444600 @default.
- W2004657461 creator A5027108774 @default.
- W2004657461 creator A5061548200 @default.
- W2004657461 creator A5067929403 @default.
- W2004657461 creator A5070859530 @default.
- W2004657461 creator A5073852389 @default.
- W2004657461 creator A5079617397 @default.
- W2004657461 creator A5083582078 @default.
- W2004657461 date "1998-12-07" @default.
- W2004657461 modified "2023-09-25" @default.
- W2004657461 title "High-performance liquid chromatographic separation of microcystins derivatized with a highly fluorescent dienophile" @default.
- W2004657461 doi "https://doi.org/10.1002/19970505nt5" @default.
- W2004657461 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9496379" @default.
- W2004657461 hasPublicationYear "1998" @default.
- W2004657461 type Work @default.
- W2004657461 sameAs 2004657461 @default.
- W2004657461 citedByCount "20" @default.
- W2004657461 countsByYear W20046574612013 @default.
- W2004657461 countsByYear W20046574612015 @default.
- W2004657461 countsByYear W20046574612016 @default.
- W2004657461 countsByYear W20046574612021 @default.
- W2004657461 countsByYear W20046574612022 @default.
- W2004657461 crossrefType "journal-article" @default.
- W2004657461 hasAuthorship W2004657461A5003503677 @default.
- W2004657461 hasAuthorship W2004657461A5014444600 @default.
- W2004657461 hasAuthorship W2004657461A5027108774 @default.
- W2004657461 hasAuthorship W2004657461A5061548200 @default.
- W2004657461 hasAuthorship W2004657461A5067929403 @default.
- W2004657461 hasAuthorship W2004657461A5070859530 @default.
- W2004657461 hasAuthorship W2004657461A5073852389 @default.
- W2004657461 hasAuthorship W2004657461A5079617397 @default.
- W2004657461 hasAuthorship W2004657461A5083582078 @default.
- W2004657461 hasConcept C108204754 @default.
- W2004657461 hasConcept C119128265 @default.
- W2004657461 hasConcept C121332964 @default.
- W2004657461 hasConcept C178790620 @default.
- W2004657461 hasConcept C179998833 @default.
- W2004657461 hasConcept C185592680 @default.
- W2004657461 hasConcept C2776568683 @default.
- W2004657461 hasConcept C2776923573 @default.
- W2004657461 hasConcept C2777463227 @default.
- W2004657461 hasConcept C2780400988 @default.
- W2004657461 hasConcept C2780471494 @default.
- W2004657461 hasConcept C40875361 @default.
- W2004657461 hasConcept C43617362 @default.
- W2004657461 hasConcept C62520636 @default.
- W2004657461 hasConcept C71240020 @default.
- W2004657461 hasConcept C91881484 @default.
- W2004657461 hasConceptScore W2004657461C108204754 @default.
- W2004657461 hasConceptScore W2004657461C119128265 @default.
- W2004657461 hasConceptScore W2004657461C121332964 @default.
- W2004657461 hasConceptScore W2004657461C178790620 @default.
- W2004657461 hasConceptScore W2004657461C179998833 @default.
- W2004657461 hasConceptScore W2004657461C185592680 @default.
- W2004657461 hasConceptScore W2004657461C2776568683 @default.
- W2004657461 hasConceptScore W2004657461C2776923573 @default.
- W2004657461 hasConceptScore W2004657461C2777463227 @default.
- W2004657461 hasConceptScore W2004657461C2780400988 @default.
- W2004657461 hasConceptScore W2004657461C2780471494 @default.
- W2004657461 hasConceptScore W2004657461C40875361 @default.
- W2004657461 hasConceptScore W2004657461C43617362 @default.
- W2004657461 hasConceptScore W2004657461C62520636 @default.
- W2004657461 hasConceptScore W2004657461C71240020 @default.
- W2004657461 hasConceptScore W2004657461C91881484 @default.
- W2004657461 hasIssue "5" @default.
- W2004657461 hasLocation W20046574611 @default.
- W2004657461 hasLocation W20046574612 @default.
- W2004657461 hasOpenAccess W2004657461 @default.
- W2004657461 hasPrimaryLocation W20046574611 @default.
- W2004657461 hasRelatedWork W1968781128 @default.
- W2004657461 hasRelatedWork W1976198411 @default.
- W2004657461 hasRelatedWork W2026578023 @default.
- W2004657461 hasRelatedWork W2063401993 @default.
- W2004657461 hasRelatedWork W2071445639 @default.
- W2004657461 hasRelatedWork W2084062459 @default.
- W2004657461 hasRelatedWork W2089074688 @default.
- W2004657461 hasRelatedWork W2096641431 @default.
- W2004657461 hasRelatedWork W2376519927 @default.
- W2004657461 hasRelatedWork W2398201682 @default.
- W2004657461 hasVolume "5" @default.
- W2004657461 isParatext "false" @default.
- W2004657461 isRetracted "false" @default.
- W2004657461 magId "2004657461" @default.
- W2004657461 workType "article" @default.