FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004676978> ?p ?o ?g. }

• W2004676978 endingPage "134" @default.
• W2004676978 startingPage "123" @default.
• W2004676978 abstract "The promoter for the leuV tRNA operon of Escherichia coli has been studied. Derivatives of this promoter were examined in vivo, fused to the cat gene or to the lacZ gene. When compared to other promoters, the leu V promoter was found to be at least three times stronger than the tyrT promoter (for the tyrT tRNA operon), or the tac promoter (trp : : lac promoter fusion) and as strong as the P1,p2 promoters of the rrnB operon (a ribosomal RNA operon). Deletion analysis revealed that, while removal of sequences downstream from +11 (relative to the transcription start point) did not affect activity, removal of sequences upstream from −39 resulted in a ten-fold reduction in expression. Unlike rRNA operons which also display upstream activation, sequences responsible for this effect in the leuV promoter are separated into two regions, one between −76 and −47, and the other between −45 and −39. DNA fragments carrying the leuV promoter migrate aberrantly on polyacrylamide gels, a phenomenon usually associated with DNA bending. One sequence thought to be involved in bending is a TTTTT run centered around −71. Point mutations engineered at this T5 region resulted in a loss of activation but had no apparent effect on migration rate. Transcription efficiency of promoter derivatives was examined in vitro using supercoiled, relaxed, or linearized plasmids as templates. Upstream activation was observed only when using relaxed templates, although maximum activity was obtained using supercoiled forms. Insertion of the very efficient 16S transcription terminator between the leuV promoter and the cat gene resulted in barely detectable activities, indicating that no antitermination mechanism was present." @default.
• W2004676978 created "2016-06-24" @default.
• W2004676978 creator A5005105444 @default.
• W2004676978 creator A5028845289 @default.
• W2004676978 creator A5054178057 @default.
• W2004676978 creator A5067633027 @default.
• W2004676978 date "1988-03-01" @default.
• W2004676978 modified "2023-09-25" @default.
• W2004676978 title "Sequence determinants for promoter strength in the leuV operon of Escherichia coli" @default.
• W2004676978 cites W116193106 @default.
• W2004676978 cites W1577430927 @default.
• W2004676978 cites W1604452613 @default.
• W2004676978 cites W1606423922 @default.
• W2004676978 cites W1619411151 @default.
• W2004676978 cites W1898646959 @default.
• W2004676978 cites W1963658914 @default.
• W2004676978 cites W1974176591 @default.
• W2004676978 cites W2005037829 @default.
• W2004676978 cites W2010434777 @default.
• W2004676978 cites W2019678147 @default.
• W2004676978 cites W2020026065 @default.
• W2004676978 cites W2031121939 @default.
• W2004676978 cites W2052200343 @default.
• W2004676978 cites W2060619293 @default.
• W2004676978 cites W2071424950 @default.
• W2004676978 cites W2103010047 @default.
• W2004676978 cites W2105439186 @default.
• W2004676978 cites W2161973127 @default.
• W2004676978 cites W2170710432 @default.
• W2004676978 cites W4239844736 @default.
• W2004676978 cites W4293247451 @default.
• W2004676978 doi "https://doi.org/10.1016/0378-1119(88)90551-3" @default.
• W2004676978 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2454871" @default.
• W2004676978 hasPublicationYear "1988" @default.
• W2004676978 type Work @default.
• W2004676978 sameAs 2004676978 @default.
• W2004676978 citedByCount "53" @default.
• W2004676978 countsByYear W20046769782022 @default.
• W2004676978 crossrefType "journal-article" @default.
• W2004676978 hasAuthorship W2004676978A5005105444 @default.
• W2004676978 hasAuthorship W2004676978A5028845289 @default.
• W2004676978 hasAuthorship W2004676978A5054178057 @default.
• W2004676978 hasAuthorship W2004676978A5067633027 @default.
• W2004676978 hasConcept C101762097 @default.
• W2004676978 hasConcept C104317684 @default.
• W2004676978 hasConcept C116403925 @default.
• W2004676978 hasConcept C121332964 @default.
• W2004676978 hasConcept C1276947 @default.
• W2004676978 hasConcept C138885662 @default.
• W2004676978 hasConcept C150194340 @default.
• W2004676978 hasConcept C153911025 @default.
• W2004676978 hasConcept C158456008 @default.
• W2004676978 hasConcept C170344550 @default.
• W2004676978 hasConcept C178046730 @default.
• W2004676978 hasConcept C179926584 @default.
• W2004676978 hasConcept C192245184 @default.
• W2004676978 hasConcept C203075996 @default.
• W2004676978 hasConcept C22744801 @default.
• W2004676978 hasConcept C37150987 @default.
• W2004676978 hasConcept C41895202 @default.
• W2004676978 hasConcept C54355233 @default.
• W2004676978 hasConcept C547475151 @default.
• W2004676978 hasConcept C552990157 @default.
• W2004676978 hasConcept C73573662 @default.
• W2004676978 hasConcept C86803240 @default.
• W2004676978 hasConceptScore W2004676978C101762097 @default.
• W2004676978 hasConceptScore W2004676978C104317684 @default.
• W2004676978 hasConceptScore W2004676978C116403925 @default.
• W2004676978 hasConceptScore W2004676978C121332964 @default.
• W2004676978 hasConceptScore W2004676978C1276947 @default.
• W2004676978 hasConceptScore W2004676978C138885662 @default.
• W2004676978 hasConceptScore W2004676978C150194340 @default.
• W2004676978 hasConceptScore W2004676978C153911025 @default.
• W2004676978 hasConceptScore W2004676978C158456008 @default.
• W2004676978 hasConceptScore W2004676978C170344550 @default.
• W2004676978 hasConceptScore W2004676978C178046730 @default.
• W2004676978 hasConceptScore W2004676978C179926584 @default.
• W2004676978 hasConceptScore W2004676978C192245184 @default.
• W2004676978 hasConceptScore W2004676978C203075996 @default.
• W2004676978 hasConceptScore W2004676978C22744801 @default.
• W2004676978 hasConceptScore W2004676978C37150987 @default.
• W2004676978 hasConceptScore W2004676978C41895202 @default.
• W2004676978 hasConceptScore W2004676978C54355233 @default.
• W2004676978 hasConceptScore W2004676978C547475151 @default.
• W2004676978 hasConceptScore W2004676978C552990157 @default.
• W2004676978 hasConceptScore W2004676978C73573662 @default.
• W2004676978 hasConceptScore W2004676978C86803240 @default.
• W2004676978 hasIssue "1" @default.
• W2004676978 hasLocation W20046769781 @default.
• W2004676978 hasLocation W20046769782 @default.
• W2004676978 hasOpenAccess W2004676978 @default.
• W2004676978 hasPrimaryLocation W20046769781 @default.
• W2004676978 hasRelatedWork W1488640901 @default.
• W2004676978 hasRelatedWork W1748608254 @default.
• W2004676978 hasRelatedWork W1965117944 @default.
• W2004676978 hasRelatedWork W1976581212 @default.
• W2004676978 hasRelatedWork W1985729581 @default.
• W2004676978 hasRelatedWork W2004676978 @default.

• next