Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004681646> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2004681646 endingPage "3018" @default.
- W2004681646 startingPage "3010" @default.
- W2004681646 abstract "A new methodology for distinguishing between specific and nonspecific protein−ligand complexes in nanoelectrospray ionization mass spectrometry (nanoES-MS) is described. The method involves the addition of an appropriate reference protein (Pref), which does not bind specifically to any of the solution components, to the nanoES solution containing the protein(s) and ligand(s) of interest. The occurrence of nonspecific protein−ligand binding is monitored by the appearance of nonspecific (Pref + ligand) complexes in the nanoES mass spectrum. Furthermore, the fraction of Pref undergoing nonspecific ligand binding provides a quantitative measure of the contribution of nonspecific binding to the measured intensities of protein and specific protein−ligand complexes. As a result, errors introduced into protein−ligand association constants, Kassoc, as determined with nanoES-MS, by nonspecific ligand binding can be corrected. The principal assumptions on which this methodology is based, namely, that the fraction of proteins and protein complexes that engage in nonspecific ligand binding during the nanoES process is determined by the number of free ligand molecules in the offspring droplets leading to gaseous ions and is independent of the size and structure of the protein or protein complex, are shown to be generally valid. The application of the method for the determination of Kassocfor two protein−carbohydrate complexes, under conditions where nonspecific ligand binding is prevalent, is demonstrated." @default.
- W2004681646 created "2016-06-24" @default.
- W2004681646 creator A5028138921 @default.
- W2004681646 creator A5029998395 @default.
- W2004681646 creator A5031933210 @default.
- W2004681646 creator A5050257402 @default.
- W2004681646 date "2006-04-01" @default.
- W2004681646 modified "2023-10-18" @default.
- W2004681646 title "Method for Distinguishing Specific from Nonspecific Protein−Ligand Complexes in Nanoelectrospray Ionization Mass Spectrometry" @default.
- W2004681646 cites W1554992716 @default.
- W2004681646 cites W1721114131 @default.
- W2004681646 cites W1970401341 @default.
- W2004681646 cites W1974732006 @default.
- W2004681646 cites W1974959933 @default.
- W2004681646 cites W1990219361 @default.
- W2004681646 cites W2000006041 @default.
- W2004681646 cites W2009247981 @default.
- W2004681646 cites W2024477808 @default.
- W2004681646 cites W2072466258 @default.
- W2004681646 cites W2083635557 @default.
- W2004681646 cites W2085986709 @default.
- W2004681646 cites W2089717057 @default.
- W2004681646 cites W967969372 @default.
- W2004681646 doi "https://doi.org/10.1021/ac0522005" @default.
- W2004681646 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16642987" @default.
- W2004681646 hasPublicationYear "2006" @default.
- W2004681646 type Work @default.
- W2004681646 sameAs 2004681646 @default.
- W2004681646 citedByCount "147" @default.
- W2004681646 countsByYear W20046816462012 @default.
- W2004681646 countsByYear W20046816462013 @default.
- W2004681646 countsByYear W20046816462014 @default.
- W2004681646 countsByYear W20046816462015 @default.
- W2004681646 countsByYear W20046816462016 @default.
- W2004681646 countsByYear W20046816462017 @default.
- W2004681646 countsByYear W20046816462018 @default.
- W2004681646 countsByYear W20046816462019 @default.
- W2004681646 countsByYear W20046816462020 @default.
- W2004681646 countsByYear W20046816462021 @default.
- W2004681646 countsByYear W20046816462022 @default.
- W2004681646 countsByYear W20046816462023 @default.
- W2004681646 crossrefType "journal-article" @default.
- W2004681646 hasAuthorship W2004681646A5028138921 @default.
- W2004681646 hasAuthorship W2004681646A5029998395 @default.
- W2004681646 hasAuthorship W2004681646A5031933210 @default.
- W2004681646 hasAuthorship W2004681646A5050257402 @default.
- W2004681646 hasConcept C109095088 @default.
- W2004681646 hasConcept C116569031 @default.
- W2004681646 hasConcept C162356407 @default.
- W2004681646 hasConcept C170493617 @default.
- W2004681646 hasConcept C185592680 @default.
- W2004681646 hasConcept C43617362 @default.
- W2004681646 hasConcept C55493867 @default.
- W2004681646 hasConcept C85196483 @default.
- W2004681646 hasConceptScore W2004681646C109095088 @default.
- W2004681646 hasConceptScore W2004681646C116569031 @default.
- W2004681646 hasConceptScore W2004681646C162356407 @default.
- W2004681646 hasConceptScore W2004681646C170493617 @default.
- W2004681646 hasConceptScore W2004681646C185592680 @default.
- W2004681646 hasConceptScore W2004681646C43617362 @default.
- W2004681646 hasConceptScore W2004681646C55493867 @default.
- W2004681646 hasConceptScore W2004681646C85196483 @default.
- W2004681646 hasIssue "9" @default.
- W2004681646 hasLocation W20046816461 @default.
- W2004681646 hasLocation W20046816462 @default.
- W2004681646 hasOpenAccess W2004681646 @default.
- W2004681646 hasPrimaryLocation W20046816461 @default.
- W2004681646 hasRelatedWork W1980377213 @default.
- W2004681646 hasRelatedWork W1999223590 @default.
- W2004681646 hasRelatedWork W2003174742 @default.
- W2004681646 hasRelatedWork W2010370934 @default.
- W2004681646 hasRelatedWork W2019498801 @default.
- W2004681646 hasRelatedWork W2043901743 @default.
- W2004681646 hasRelatedWork W2050262667 @default.
- W2004681646 hasRelatedWork W2271539130 @default.
- W2004681646 hasRelatedWork W2739417245 @default.
- W2004681646 hasRelatedWork W2952244581 @default.
- W2004681646 hasVolume "78" @default.
- W2004681646 isParatext "false" @default.
- W2004681646 isRetracted "false" @default.
- W2004681646 magId "2004681646" @default.
- W2004681646 workType "article" @default.