Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004687265> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2004687265 abstract "A commercial use of microbial produced products, like polyhydroxyalkanotes (PHAs), in the sense of an environmental precaution appears meaningful and necessary. In order to more economically produce microbial products, this investigation was focused on suitable producers, like the yeast Schizosaccharomyces pombe. Since it is not capable of the PHA synthesis, easily cultured and they must be modified genetically. Therefore, the genes of thePHB biosynthesis pathway of Ralstonia eutropha [beta-ketothiolase (phbARe); acetoacetyl-CoA reductase (phbBRe); as well as PHB synthase (phbCRe), located onto the plasmid pBHR68 were cloned into the cohesive ended pYIplac128 integrated vector that transformed into the chromosome of the yeast Schizosaccharomyces pombe strain Q01. Under the optimized cultivation conditions, the transgenic yeast S. pombe strain Q01/PHB was able to produce PHB and accumulated up to 9.018 % PHB. The presence of heterologous DNA in the transgenic yeast was examined by means of Western blot analysis. In addition, both PHA synthase activity and kinetics were determined. The UV/Vis, 1H and 13C NMR spectral analysis have confirmed that the polymer produced by the yeast S. pombe strain Q01/PHB is a pure homopolymer of 3-hydroxybutyric acid" @default.
- W2004687265 created "2016-06-24" @default.
- W2004687265 creator A5042198381 @default.
- W2004687265 creator A5047144095 @default.
- W2004687265 creator A5079084447 @default.
- W2004687265 creator A5082684072 @default.
- W2004687265 creator A5091289682 @default.
- W2004687265 date "2010-06-01" @default.
- W2004687265 modified "2023-10-01" @default.
- W2004687265 title "Genetic engineering of <i.Schizosaccharomyces pombe</i> to produce Bacterial Polyhydroxyalkanotes" @default.
- W2004687265 doi "https://doi.org/10.4314/jasem.v11i2.54996" @default.
- W2004687265 hasPublicationYear "2010" @default.
- W2004687265 type Work @default.
- W2004687265 sameAs 2004687265 @default.
- W2004687265 citedByCount "0" @default.
- W2004687265 crossrefType "journal-article" @default.
- W2004687265 hasAuthorship W2004687265A5042198381 @default.
- W2004687265 hasAuthorship W2004687265A5047144095 @default.
- W2004687265 hasAuthorship W2004687265A5079084447 @default.
- W2004687265 hasAuthorship W2004687265A5082684072 @default.
- W2004687265 hasAuthorship W2004687265A5091289682 @default.
- W2004687265 hasBestOaLocation W20046872651 @default.
- W2004687265 hasConcept C104317684 @default.
- W2004687265 hasConcept C105702510 @default.
- W2004687265 hasConcept C108305142 @default.
- W2004687265 hasConcept C112243037 @default.
- W2004687265 hasConcept C185592680 @default.
- W2004687265 hasConcept C22744801 @default.
- W2004687265 hasConcept C2777576037 @default.
- W2004687265 hasConcept C2778022156 @default.
- W2004687265 hasConcept C2779222958 @default.
- W2004687265 hasConcept C2779429346 @default.
- W2004687265 hasConcept C2780734198 @default.
- W2004687265 hasConcept C40767141 @default.
- W2004687265 hasConcept C55493867 @default.
- W2004687265 hasConcept C86803240 @default.
- W2004687265 hasConceptScore W2004687265C104317684 @default.
- W2004687265 hasConceptScore W2004687265C105702510 @default.
- W2004687265 hasConceptScore W2004687265C108305142 @default.
- W2004687265 hasConceptScore W2004687265C112243037 @default.
- W2004687265 hasConceptScore W2004687265C185592680 @default.
- W2004687265 hasConceptScore W2004687265C22744801 @default.
- W2004687265 hasConceptScore W2004687265C2777576037 @default.
- W2004687265 hasConceptScore W2004687265C2778022156 @default.
- W2004687265 hasConceptScore W2004687265C2779222958 @default.
- W2004687265 hasConceptScore W2004687265C2779429346 @default.
- W2004687265 hasConceptScore W2004687265C2780734198 @default.
- W2004687265 hasConceptScore W2004687265C40767141 @default.
- W2004687265 hasConceptScore W2004687265C55493867 @default.
- W2004687265 hasConceptScore W2004687265C86803240 @default.
- W2004687265 hasIssue "2" @default.
- W2004687265 hasLocation W20046872651 @default.
- W2004687265 hasOpenAccess W2004687265 @default.
- W2004687265 hasPrimaryLocation W20046872651 @default.
- W2004687265 hasRelatedWork W1666948189 @default.
- W2004687265 hasRelatedWork W1917962375 @default.
- W2004687265 hasRelatedWork W1970610961 @default.
- W2004687265 hasRelatedWork W2004687265 @default.
- W2004687265 hasRelatedWork W2023700098 @default.
- W2004687265 hasRelatedWork W2037819725 @default.
- W2004687265 hasRelatedWork W2139132507 @default.
- W2004687265 hasRelatedWork W2147046886 @default.
- W2004687265 hasRelatedWork W2171263406 @default.
- W2004687265 hasRelatedWork W1971173882 @default.
- W2004687265 hasVolume "11" @default.
- W2004687265 isParatext "false" @default.
- W2004687265 isRetracted "false" @default.
- W2004687265 magId "2004687265" @default.
- W2004687265 workType "article" @default.