FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004687635> ?p ?o ?g. }

• W2004687635 endingPage "226" @default.
• W2004687635 startingPage "216" @default.
• W2004687635 abstract "Summary Cationic liposomes are being used increasingly as efficient adjuvants for subunit vaccines but their precise mechanism of action is still unknown. Here, we investigated the adjuvant mechanism of cationic liposomes based on the synthetic amphiphile dimethyldioctadecylammonium (DDA). The liposomes did not have an effect on the maturation of murine bone‐marrow‐derived dendritic cells (BM‐DCs) related to the surface expression of major histocompatibility complex (MHC) class II, CD40, CD80 and CD86. We found that ovalbumin (OVA) readily associated with the liposomes (> 90%) when mixed in equal concentrations. This efficient adsorption onto the liposomes led to an enhanced uptake of OVA by BM‐DCs as assessed by flow cytometry and confocal fluorescence laser‐scanning microscopy. This was an active process, which was arrested at 4° and by an inhibitor of actin‐dependent endocytosis, cytochalasin D. In vivo studies confirmed the observed effect because adsorption of OVA onto DDA liposomes enhanced the uptake of the antigen by peritoneal exudate cells after intraperitoneal injection. The liposomes targeted antigen preferentially to antigen‐presenting cells because we only observed a minimal uptake by T cells in mixed splenocyte cultures. The adsorption of antigen onto the liposomes increased the efficiency of antigen presentation more than 100 times in a responder assay with MHC class II‐restricted OVA‐specific T‐cell receptor transgenic DO11.10 T cells. Our data therefore suggest that the primary adjuvant mechanism of cationic DDA liposomes is to target the cell membrane of antigen‐presenting cells, which subsequently leads to enhanced uptake and presentation of antigen." @default.
• W2004687635 created "2016-06-24" @default.
• W2004687635 creator A5028590821 @default.
• W2004687635 creator A5034593036 @default.
• W2004687635 creator A5036098449 @default.
• W2004687635 creator A5042180788 @default.
• W2004687635 creator A5060122927 @default.
• W2004687635 creator A5070337709 @default.
• W2004687635 creator A5088726380 @default.
• W2004687635 creator A5090428914 @default.
• W2004687635 date "2007-02-14" @default.
• W2004687635 modified "2023-10-15" @default.
• W2004687635 title "The adjuvant mechanism of cationic dimethyldioctadecylammonium liposomes" @default.
• W2004687635 cites W1479986126 @default.
• W2004687635 cites W1593707348 @default.
• W2004687635 cites W1635217668 @default.
• W2004687635 cites W1661544713 @default.
• W2004687635 cites W1668154572 @default.
• W2004687635 cites W1949623051 @default.
• W2004687635 cites W1967994686 @default.
• W2004687635 cites W1968113316 @default.
• W2004687635 cites W1971290197 @default.
• W2004687635 cites W1975499385 @default.
• W2004687635 cites W1975833492 @default.
• W2004687635 cites W1976102012 @default.
• W2004687635 cites W1976811878 @default.
• W2004687635 cites W2002945470 @default.
• W2004687635 cites W2007481474 @default.
• W2004687635 cites W2018289835 @default.
• W2004687635 cites W2018544077 @default.
• W2004687635 cites W2018638665 @default.
• W2004687635 cites W2036331194 @default.
• W2004687635 cites W2043560018 @default.
• W2004687635 cites W2044853699 @default.
• W2004687635 cites W2044910913 @default.
• W2004687635 cites W2049862985 @default.
• W2004687635 cites W2052211731 @default.
• W2004687635 cites W2061622709 @default.
• W2004687635 cites W2072074997 @default.
• W2004687635 cites W2074531540 @default.
• W2004687635 cites W2076037123 @default.
• W2004687635 cites W2077660894 @default.
• W2004687635 cites W2085530908 @default.
• W2004687635 cites W2089716915 @default.
• W2004687635 cites W2091955078 @default.
• W2004687635 cites W2098376804 @default.
• W2004687635 cites W2099389359 @default.
• W2004687635 cites W2100837269 @default.
• W2004687635 cites W2105277037 @default.
• W2004687635 cites W2113972541 @default.
• W2004687635 cites W2116683264 @default.
• W2004687635 cites W2118835251 @default.
• W2004687635 cites W2120226980 @default.
• W2004687635 cites W2126883459 @default.
• W2004687635 cites W2137613681 @default.
• W2004687635 cites W2143875501 @default.
• W2004687635 cites W2156122217 @default.
• W2004687635 cites W2158204548 @default.
• W2004687635 cites W2158785107 @default.
• W2004687635 cites W2160670356 @default.
• W2004687635 cites W2161929802 @default.
• W2004687635 cites W2163964526 @default.
• W2004687635 cites W2166013785 @default.
• W2004687635 cites W2168466854 @default.
• W2004687635 cites W2183579169 @default.
• W2004687635 cites W2323505647 @default.
• W2004687635 cites W4253747558 @default.
• W2004687635 cites W4256309019 @default.
• W2004687635 cites W4313341466 @default.
• W2004687635 doi "https://doi.org/10.1111/j.1365-2567.2007.02560.x" @default.
• W2004687635 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2265938" @default.
• W2004687635 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17302734" @default.
• W2004687635 hasPublicationYear "2007" @default.
• W2004687635 type Work @default.
• W2004687635 sameAs 2004687635 @default.
• W2004687635 citedByCount "162" @default.
• W2004687635 countsByYear W20046876352012 @default.
• W2004687635 countsByYear W20046876352013 @default.
• W2004687635 countsByYear W20046876352014 @default.
• W2004687635 countsByYear W20046876352015 @default.
• W2004687635 countsByYear W20046876352016 @default.
• W2004687635 countsByYear W20046876352017 @default.
• W2004687635 countsByYear W20046876352018 @default.
• W2004687635 countsByYear W20046876352019 @default.
• W2004687635 countsByYear W20046876352020 @default.
• W2004687635 countsByYear W20046876352021 @default.
• W2004687635 countsByYear W20046876352022 @default.
• W2004687635 countsByYear W20046876352023 @default.
• W2004687635 crossrefType "journal-article" @default.
• W2004687635 hasAuthorship W2004687635A5028590821 @default.
• W2004687635 hasAuthorship W2004687635A5034593036 @default.
• W2004687635 hasAuthorship W2004687635A5036098449 @default.
• W2004687635 hasAuthorship W2004687635A5042180788 @default.
• W2004687635 hasAuthorship W2004687635A5060122927 @default.
• W2004687635 hasAuthorship W2004687635A5070337709 @default.
• W2004687635 hasAuthorship W2004687635A5088726380 @default.
• W2004687635 hasAuthorship W2004687635A5090428914 @default.
• W2004687635 hasBestOaLocation W20046876352 @default.

• next