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- W2004703609 abstract "Abstract The complete utilization of raffinose and melibiose by Saccharomyces cerevisiae requires the synthesis and secretion of an α-galactosidase encoded by a single MEL1 gene. The influence of culture conditions on the productivity of α-galactosidase in batch growth of yeast cells has been investigated. A modulation of the MEL1 gene expression has been observed depending on growth condition, with a maximum of enzyme productivity during a transient physiological phase corresponding to the early stationary phase. Our experiments demonstrate that the highest α-galactosidase productivity observed in the early stationary phase of growth is not associated with a starvation condition, but it is coupled to a particular growth phase. The increase of specific activity of the enzyme occurs mainly for periplasmic and intracellular fractions while the amount of enzyme secreted in the medium remains fairly constant." @default.
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- W2004703609 date "1989-10-01" @default.
- W2004703609 modified "2023-09-26" @default.
- W2004703609 title "Growth phase modulation of the productivity of α-galactosidase in budding yeast cultures" @default.
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- W2004703609 doi "https://doi.org/10.1016/0168-1656(89)90130-2" @default.
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