Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004706064> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2004706064 endingPage "582" @default.
- W2004706064 startingPage "575" @default.
- W2004706064 abstract "Abstract Fluorescent Nitric Oxide Cheletropic Traps (FNOCTs) were applied to specifically trap nitric oxide (NO) with high sensitivity. The fluorescent oquinoid ?electron system of the FNOCTs (? = 460 nm, ? = 600 nm) reacts rapidly with NO to a fluorescent phenanthrene system (? = 380 nm, ? = 460 nm). The cyclic nitroxides thus formed react further to nonradical products which exhibit identical fluorescence properties. Using the acid form of the trap (FNOCT-4), NO release by spermine NONOate and by lipopolysaccharide (LPS) activated alveolar macrophages were studied. A maximum extracellular release of NO of 37.5 nmol h[-1] (10[6] cells)[-1] from the macrophages was determined at 11 h after activation. Furthermore, intracellular NO release by LPSactivated macrophages and by microvascular omentum endothelial cells stimulated by the Ca[2+] ionophore A-23187, respectively, was monitored on the single cell level by means of fluorescence microscopy. After loading the cells with the membranepermeating acetoxymethylester derivative FNOCT-5,which is hydrolyzed to a nonpermeating dicarboxylate by intracellular hydrolases, NO formation by the endothelial cells started immediately upon stimulation, whereas start of NO production by the macrophages was delayed with a variation between 4 and 8 h for individual cells. These results demonstrate that the FNOCTs can be used to monitor NO release from single cells, as well as from NOdonating compounds, with high sensitivity and with temporal and spatial resolution." @default.
- W2004706064 created "2016-06-24" @default.
- W2004706064 creator A5005896077 @default.
- W2004706064 creator A5008111110 @default.
- W2004706064 creator A5014160414 @default.
- W2004706064 creator A5019959116 @default.
- W2004706064 creator A5032840577 @default.
- W2004706064 date "2000-07-04" @default.
- W2004706064 modified "2023-10-17" @default.
- W2004706064 title "Nitric Oxide Detection and Visualization in Biological Systems. Applications of the FNOCT Method" @default.
- W2004706064 cites W1980528903 @default.
- W2004706064 cites W1983189786 @default.
- W2004706064 cites W1990802251 @default.
- W2004706064 cites W2010055809 @default.
- W2004706064 cites W2027602169 @default.
- W2004706064 cites W2042681695 @default.
- W2004706064 cites W2121198758 @default.
- W2004706064 cites W2155579775 @default.
- W2004706064 cites W2171927855 @default.
- W2004706064 doi "https://doi.org/10.1515/bc.2000.074" @default.
- W2004706064 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10987364" @default.
- W2004706064 hasPublicationYear "2000" @default.
- W2004706064 type Work @default.
- W2004706064 sameAs 2004706064 @default.
- W2004706064 citedByCount "31" @default.
- W2004706064 countsByYear W20047060642012 @default.
- W2004706064 countsByYear W20047060642013 @default.
- W2004706064 countsByYear W20047060642014 @default.
- W2004706064 countsByYear W20047060642022 @default.
- W2004706064 crossrefType "journal-article" @default.
- W2004706064 hasAuthorship W2004706064A5005896077 @default.
- W2004706064 hasAuthorship W2004706064A5008111110 @default.
- W2004706064 hasAuthorship W2004706064A5014160414 @default.
- W2004706064 hasAuthorship W2004706064A5019959116 @default.
- W2004706064 hasAuthorship W2004706064A5032840577 @default.
- W2004706064 hasConcept C121332964 @default.
- W2004706064 hasConcept C12554922 @default.
- W2004706064 hasConcept C169274487 @default.
- W2004706064 hasConcept C169760540 @default.
- W2004706064 hasConcept C178790620 @default.
- W2004706064 hasConcept C185592680 @default.
- W2004706064 hasConcept C203014093 @default.
- W2004706064 hasConcept C24998067 @default.
- W2004706064 hasConcept C2778754761 @default.
- W2004706064 hasConcept C2779014961 @default.
- W2004706064 hasConcept C28406088 @default.
- W2004706064 hasConcept C41625074 @default.
- W2004706064 hasConcept C519581460 @default.
- W2004706064 hasConcept C55493867 @default.
- W2004706064 hasConcept C62520636 @default.
- W2004706064 hasConcept C79879829 @default.
- W2004706064 hasConcept C86803240 @default.
- W2004706064 hasConcept C91881484 @default.
- W2004706064 hasConceptScore W2004706064C121332964 @default.
- W2004706064 hasConceptScore W2004706064C12554922 @default.
- W2004706064 hasConceptScore W2004706064C169274487 @default.
- W2004706064 hasConceptScore W2004706064C169760540 @default.
- W2004706064 hasConceptScore W2004706064C178790620 @default.
- W2004706064 hasConceptScore W2004706064C185592680 @default.
- W2004706064 hasConceptScore W2004706064C203014093 @default.
- W2004706064 hasConceptScore W2004706064C24998067 @default.
- W2004706064 hasConceptScore W2004706064C2778754761 @default.
- W2004706064 hasConceptScore W2004706064C2779014961 @default.
- W2004706064 hasConceptScore W2004706064C28406088 @default.
- W2004706064 hasConceptScore W2004706064C41625074 @default.
- W2004706064 hasConceptScore W2004706064C519581460 @default.
- W2004706064 hasConceptScore W2004706064C55493867 @default.
- W2004706064 hasConceptScore W2004706064C62520636 @default.
- W2004706064 hasConceptScore W2004706064C79879829 @default.
- W2004706064 hasConceptScore W2004706064C86803240 @default.
- W2004706064 hasConceptScore W2004706064C91881484 @default.
- W2004706064 hasIssue "7" @default.
- W2004706064 hasLocation W20047060641 @default.
- W2004706064 hasLocation W20047060642 @default.
- W2004706064 hasOpenAccess W2004706064 @default.
- W2004706064 hasPrimaryLocation W20047060641 @default.
- W2004706064 hasRelatedWork W1972445264 @default.
- W2004706064 hasRelatedWork W1983842579 @default.
- W2004706064 hasRelatedWork W1985900017 @default.
- W2004706064 hasRelatedWork W1997641504 @default.
- W2004706064 hasRelatedWork W1998362519 @default.
- W2004706064 hasRelatedWork W2011918669 @default.
- W2004706064 hasRelatedWork W2386408983 @default.
- W2004706064 hasRelatedWork W2565098243 @default.
- W2004706064 hasRelatedWork W616704223 @default.
- W2004706064 hasRelatedWork W940006813 @default.
- W2004706064 hasVolume "381" @default.
- W2004706064 isParatext "false" @default.
- W2004706064 isRetracted "false" @default.
- W2004706064 magId "2004706064" @default.
- W2004706064 workType "article" @default.