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• W2004725139 abstract "The optical spectroscopic properties of 1,4(and 5)-benzenedicarboxylic acid, 2-[10-(dimethylamino)-4-fluoro-3-oxo-3H-benzo[c]xanthen-7-yl] (carboxy SNARF-4F), a commercial promising fluorescent pH probe, are investigated in buffered aqueous solutions in the 5.6−8.2 pH range by steady-state and time-resolved spectroscopy. A multiexponential global analysis of the picosecond time-resolved data is performed. The nonprotonated A* species decays monoexponentially (0.73 ns), while the protonated species AH* decays following a biexponential law with time constants of 0.40 and 1.87 ns. A kinetic scheme is proposed to explain the observations, which involves AH* in equilibrium with a species denoted Y*. The nature of Y* is discussed in terms of a possible structural change in the molecule producing the lactone form, although the formation of a hydrogen-bonded complex to the solvent cannot be ruled out. Finally, the ability of the dye to probe pH at the single-molecule level is explored using fluorescence correlation spectroscopy." @default.
• W2004725139 created "2016-06-24" @default.
• W2004725139 creator A5049857216 @default.
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• W2004725139 date "2005-05-20" @default.
• W2004725139 modified "2023-10-18" @default.
• W2004725139 title "Carboxy SNARF-4F as a Fluorescent pH Probe for Ensemble and Fluorescence Correlation Spectroscopies" @default.
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• W2004725139 doi "https://doi.org/10.1021/jp0510138" @default.
• W2004725139 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16852451" @default.
• W2004725139 hasPublicationYear "2005" @default.
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