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• W2004746837 abstract "This report introduces a new analytical concept utilizing the mass transfer resistance of a liquid–liquid interface to concentrate and separate DNA samples. DNA molecules can be electrophoretically accumulated at a liquid–liquid interface of an aqueous two-phase system (ATPS) of poly(ethylene glycol) (PEG) and dextran, two polymers that form two immiscible phases in aqueous electrolyte solutions. The detachment of DNA from the interface into the other phase can be triggered by increasing the applied electric field. We experimentally study the size dependence of the detachment process for a broad spectrum of DNA fragments. In a regime where the coiling of the chains does not play a significant role, the process shows a linear dependence on the diffusion coefficient, with shorter DNA chains detaching at lower electric field strengths than larger ones. The concept may enable novel separation protocols for preparative and analytical purposes." @default.
• W2004746837 created "2016-06-24" @default.
• W2004746837 creator A5013628640 @default.
• W2004746837 creator A5039743851 @default.
• W2004746837 date "2011-06-22" @default.
• W2004746837 modified "2023-10-03" @default.
• W2004746837 title "Concentration and Size Separation of DNA Samples at Liquid–Liquid Interfaces" @default.
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• W2004746837 doi "https://doi.org/10.1021/ac201228v" @default.
• W2004746837 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21682284" @default.
• W2004746837 hasPublicationYear "2011" @default.
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