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- W2004755241 abstract "A new method is suggested here for determining the amino acid sequence of proteins from the mass spectrum of a single solution. A computer simulation of the reconstruction of the sequence from ideal mass-spectrometer data is presented. The method requires first the random fragmentation of a protein, as by partial acid hydrolysis. Volatile derivatives of the fragments, still mixed together, must next be made. The mass measurements are performed on the mixture of volatile derivatives. All the required chemical treatments would be simple and of such nature that the whole analysis, beginning with a sample of purified protein and ending with the computer printout of the amino acid sequences, could be made rapidly. The required amount of volatile derivative protein sample is expected to be very small, of the order of 1 mg. This should make feasible various experiments, for example with single organisms which are impossible at present." @default.
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- W2004755241 modified "2023-09-23" @default.
- W2004755241 title "MASSPEC: A computer program for complete sequence analysis of large proteins from mass spectrometry data of a single sample" @default.
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- W2004755241 doi "https://doi.org/10.1016/0010-4825(70)90013-2" @default.
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