FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004789944> ?p ?o ?g. }

• W2004789944 endingPage "124" @default.
• W2004789944 startingPage "114" @default.
• W2004789944 abstract "We describe an enzyme-linked immunosorbent assay (ELISA) for quantifying relative amounts of active caspase 3 in apoptotic cells. Covalent modification of caspase 3 active sites with a biotinylated inhibitor differentiates active from latent caspases. Capture on an ELISA plate with an antibody specific for caspase 3 makes the assay specific for caspase 3. Detection is with horseradish peroxidase (HRP)-conjugated streptavidin that binds to the biotinylated inhibitor covalently bound to caspase 3. Using the assay we detected 6.6 ng active caspase 3 per 10(6) apoptotic staurosporine-treated Jurkat cells. Specificity of the assay for caspase 3 was demonstrated by lack of signal with purified caspases 2, 7, 8, and 10 that were modified by a biotinylated inhibitor. Specificity was also demonstrated by lack of signal with apoptotic MCF-7 cells which do not express caspase 3. The ability to discriminate between active and latent caspase 3 was shown by Western blotting with HRP-streptavidin and anti-caspase 3. Although latent caspase 3 was captured it was not covalently modified with the biotinylated inhibitor. The basic principle of using a covalent inhibitor to identify active enzymes and an antibody to differentiate between enzymes with similar activities has potential for quantifying active members of many classes of enzymes." @default.
• W2004789944 created "2016-06-24" @default.
• W2004789944 creator A5004138291 @default.
• W2004789944 creator A5030672978 @default.
• W2004789944 creator A5031530963 @default.
• W2004789944 creator A5040904290 @default.
• W2004789944 creator A5054793504 @default.
• W2004789944 creator A5060310314 @default.
• W2004789944 creator A5062003137 @default.
• W2004789944 creator A5065595807 @default.
• W2004789944 creator A5067532178 @default.
• W2004789944 creator A5068310637 @default.
• W2004789944 creator A5069607817 @default.
• W2004789944 creator A5071102647 @default.
• W2004789944 creator A5074328241 @default.
• W2004789944 creator A5078144648 @default.
• W2004789944 creator A5079525915 @default.
• W2004789944 creator A5081030472 @default.
• W2004789944 creator A5082352549 @default.
• W2004789944 creator A5089189581 @default.
• W2004789944 date "2000-08-01" @default.
• W2004789944 modified "2023-09-27" @default.
• W2004789944 title "Quantification of Active Caspase 3 in Apoptotic Cells" @default.
• W2004789944 cites W1931472750 @default.
• W2004789944 cites W1977219317 @default.
• W2004789944 cites W1979842763 @default.
• W2004789944 cites W1987407537 @default.
• W2004789944 cites W1988043250 @default.
• W2004789944 cites W1997396825 @default.
• W2004789944 cites W2008261126 @default.
• W2004789944 cites W2010243701 @default.
• W2004789944 cites W2015608566 @default.
• W2004789944 cites W2022380058 @default.
• W2004789944 cites W2025044046 @default.
• W2004789944 cites W2025128734 @default.
• W2004789944 cites W2026692684 @default.
• W2004789944 cites W2028582699 @default.
• W2004789944 cites W2033240274 @default.
• W2004789944 cites W2039048150 @default.
• W2004789944 cites W2045309540 @default.
• W2004789944 cites W2045563337 @default.
• W2004789944 cites W2051658957 @default.
• W2004789944 cites W2061497413 @default.
• W2004789944 cites W2075226068 @default.
• W2004789944 cites W2077705257 @default.
• W2004789944 cites W2089154951 @default.
• W2004789944 cites W2105263387 @default.
• W2004789944 cites W2107237397 @default.
• W2004789944 cites W2119683782 @default.
• W2004789944 cites W2120939161 @default.
• W2004789944 cites W2138144052 @default.
• W2004789944 cites W2154571107 @default.
• W2004789944 cites W2156179144 @default.
• W2004789944 cites W2156483686 @default.
• W2004789944 cites W2157999078 @default.
• W2004789944 cites W2340925902 @default.
• W2004789944 doi "https://doi.org/10.1006/abio.2000.4690" @default.
• W2004789944 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10933864" @default.
• W2004789944 hasPublicationYear "2000" @default.
• W2004789944 type Work @default.
• W2004789944 sameAs 2004789944 @default.
• W2004789944 citedByCount "42" @default.
• W2004789944 countsByYear W20047899442012 @default.
• W2004789944 countsByYear W20047899442014 @default.
• W2004789944 countsByYear W20047899442015 @default.
• W2004789944 countsByYear W20047899442016 @default.
• W2004789944 countsByYear W20047899442021 @default.
• W2004789944 crossrefType "journal-article" @default.
• W2004789944 hasAuthorship W2004789944A5004138291 @default.
• W2004789944 hasAuthorship W2004789944A5030672978 @default.
• W2004789944 hasAuthorship W2004789944A5031530963 @default.
• W2004789944 hasAuthorship W2004789944A5040904290 @default.
• W2004789944 hasAuthorship W2004789944A5054793504 @default.
• W2004789944 hasAuthorship W2004789944A5060310314 @default.
• W2004789944 hasAuthorship W2004789944A5062003137 @default.
• W2004789944 hasAuthorship W2004789944A5065595807 @default.
• W2004789944 hasAuthorship W2004789944A5067532178 @default.
• W2004789944 hasAuthorship W2004789944A5068310637 @default.
• W2004789944 hasAuthorship W2004789944A5069607817 @default.
• W2004789944 hasAuthorship W2004789944A5071102647 @default.
• W2004789944 hasAuthorship W2004789944A5074328241 @default.
• W2004789944 hasAuthorship W2004789944A5078144648 @default.
• W2004789944 hasAuthorship W2004789944A5079525915 @default.
• W2004789944 hasAuthorship W2004789944A5081030472 @default.
• W2004789944 hasAuthorship W2004789944A5082352549 @default.
• W2004789944 hasAuthorship W2004789944A5089189581 @default.
• W2004789944 hasConcept C12519072 @default.
• W2004789944 hasConcept C153911025 @default.
• W2004789944 hasConcept C179464577 @default.
• W2004789944 hasConcept C181199279 @default.
• W2004789944 hasConcept C185592680 @default.
• W2004789944 hasConcept C190283241 @default.
• W2004789944 hasConcept C195794163 @default.
• W2004789944 hasConcept C203014093 @default.
• W2004789944 hasConcept C204628709 @default.
• W2004789944 hasConcept C2776090121 @default.
• W2004789944 hasConcept C2779084600 @default.
• W2004789944 hasConcept C31573885 @default.

• next