FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004803144> ?p ?o ?g. }

• W2004803144 endingPage "1907" @default.
• W2004803144 startingPage "1898" @default.
• W2004803144 abstract "This article describes a wide-field time-domain fluorescence lifetime imaging (FLIM) microscope with optical sectioning. The FLIM system utilizes a wide-field time-gated optical image intensifier, with a minimum gate width of 85 ps, to achieve high temporal resolution of fluorescence decays induced by ultrashort laser pulses. Different configurations, using excitation pulses of picojoule energy at 80 MHz repetition rate and of nanojoule energy at 10 kHz, are compared. The instrument has a temporal dynamic range spanning from 100 ps to tens of μs and is shown to have a temporal discrimination better than 10 ps. When applied to laser dye samples, it has produced FLIM maps demonstrating sensitivity to variations in both chemical species and local environment, e.g., viscosity. Wide-field optical sectioning is achieved using the technique of structured illumination, which is applied to remove out-of-focus light that can result in lifetime artifacts. The sectioning strength, which may be adjusted by choosing an appropriate spatial modulation frequency, is characterized and shown to be comparable to that of a confocal microscope. Practical considerations concerned with improving the quality of sectioned fluorescence lifetime maps, including using a large bit depth camera, are discussed." @default.
• W2004803144 created "2016-06-24" @default.
• W2004803144 creator A5007804636 @default.
• W2004803144 creator A5012287556 @default.
• W2004803144 creator A5020495711 @default.
• W2004803144 creator A5023702696 @default.
• W2004803144 creator A5030430194 @default.
• W2004803144 creator A5030855454 @default.
• W2004803144 creator A5035947212 @default.
• W2004803144 creator A5051388808 @default.
• W2004803144 creator A5056627546 @default.
• W2004803144 creator A5062278190 @default.
• W2004803144 creator A5071925801 @default.
• W2004803144 creator A5081508678 @default.
• W2004803144 creator A5087967694 @default.
• W2004803144 date "2002-03-27" @default.
• W2004803144 modified "2023-10-17" @default.
• W2004803144 title "A wide-field time-domain fluorescence lifetime imaging microscope with optical sectioning" @default.
• W2004803144 cites W1981564484 @default.
• W2004803144 cites W1981930358 @default.
• W2004803144 cites W1988363153 @default.
• W2004803144 cites W1991036242 @default.
• W2004803144 cites W1991565661 @default.
• W2004803144 cites W1993272328 @default.
• W2004803144 cites W2008645012 @default.
• W2004803144 cites W2017115591 @default.
• W2004803144 cites W2020556902 @default.
• W2004803144 cites W2024273569 @default.
• W2004803144 cites W2026026462 @default.
• W2004803144 cites W2038034321 @default.
• W2004803144 cites W2038042124 @default.
• W2004803144 cites W2050521993 @default.
• W2004803144 cites W2054985719 @default.
• W2004803144 cites W2060714866 @default.
• W2004803144 cites W2066736326 @default.
• W2004803144 cites W2086356224 @default.
• W2004803144 cites W2087812938 @default.
• W2004803144 cites W2104045844 @default.
• W2004803144 cites W2171196112 @default.
• W2004803144 doi "https://doi.org/10.1063/1.1458061" @default.
• W2004803144 hasPublicationYear "2002" @default.
• W2004803144 type Work @default.
• W2004803144 sameAs 2004803144 @default.
• W2004803144 citedByCount "74" @default.
• W2004803144 countsByYear W20048031442012 @default.
• W2004803144 countsByYear W20048031442013 @default.
• W2004803144 countsByYear W20048031442014 @default.
• W2004803144 countsByYear W20048031442015 @default.
• W2004803144 countsByYear W20048031442016 @default.
• W2004803144 countsByYear W20048031442017 @default.
• W2004803144 countsByYear W20048031442018 @default.
• W2004803144 countsByYear W20048031442019 @default.
• W2004803144 countsByYear W20048031442020 @default.
• W2004803144 countsByYear W20048031442021 @default.
• W2004803144 countsByYear W20048031442022 @default.
• W2004803144 countsByYear W20048031442023 @default.
• W2004803144 crossrefType "journal-article" @default.
• W2004803144 hasAuthorship W2004803144A5007804636 @default.
• W2004803144 hasAuthorship W2004803144A5012287556 @default.
• W2004803144 hasAuthorship W2004803144A5020495711 @default.
• W2004803144 hasAuthorship W2004803144A5023702696 @default.
• W2004803144 hasAuthorship W2004803144A5030430194 @default.
• W2004803144 hasAuthorship W2004803144A5030855454 @default.
• W2004803144 hasAuthorship W2004803144A5035947212 @default.
• W2004803144 hasAuthorship W2004803144A5051388808 @default.
• W2004803144 hasAuthorship W2004803144A5056627546 @default.
• W2004803144 hasAuthorship W2004803144A5062278190 @default.
• W2004803144 hasAuthorship W2004803144A5071925801 @default.
• W2004803144 hasAuthorship W2004803144A5081508678 @default.
• W2004803144 hasAuthorship W2004803144A5087967694 @default.
• W2004803144 hasConcept C119666444 @default.
• W2004803144 hasConcept C120665830 @default.
• W2004803144 hasConcept C121332964 @default.
• W2004803144 hasConcept C136009344 @default.
• W2004803144 hasConcept C147080431 @default.
• W2004803144 hasConcept C192562407 @default.
• W2004803144 hasConcept C20446124 @default.
• W2004803144 hasConcept C26771246 @default.
• W2004803144 hasConcept C520434653 @default.
• W2004803144 hasConcept C57477423 @default.
• W2004803144 hasConcept C67649825 @default.
• W2004803144 hasConcept C77017923 @default.
• W2004803144 hasConcept C91881484 @default.
• W2004803144 hasConceptScore W2004803144C119666444 @default.
• W2004803144 hasConceptScore W2004803144C120665830 @default.
• W2004803144 hasConceptScore W2004803144C121332964 @default.
• W2004803144 hasConceptScore W2004803144C136009344 @default.
• W2004803144 hasConceptScore W2004803144C147080431 @default.
• W2004803144 hasConceptScore W2004803144C192562407 @default.
• W2004803144 hasConceptScore W2004803144C20446124 @default.
• W2004803144 hasConceptScore W2004803144C26771246 @default.
• W2004803144 hasConceptScore W2004803144C520434653 @default.
• W2004803144 hasConceptScore W2004803144C57477423 @default.
• W2004803144 hasConceptScore W2004803144C67649825 @default.
• W2004803144 hasConceptScore W2004803144C77017923 @default.
• W2004803144 hasConceptScore W2004803144C91881484 @default.
• W2004803144 hasIssue "4" @default.
• W2004803144 hasLocation W20048031441 @default.

• next