Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004809474> ?p ?o ?g. }
- W2004809474 endingPage "371" @default.
- W2004809474 startingPage "361" @default.
- W2004809474 abstract "Although the tubulysin (tub) biosynthetic gene cluster has been located in two myxobacterial strains, it appears in both cases to be incomplete as obvious candidates for acyl transfer and oxidation functions are lacking. Here, we report the engineering of a heterologous expression system for the tub biosynthetic pathway from strain Cystobacter sp. SBCb004. The entire tub core cluster was reconstituted from two cosmids using Red/ET recombineering and heterologous expression achieved in strains Pseudomonas putida and Myxococcus xanthus. Availability of the heterologous expression system and the natural producer strain SBCb004 provided a platform for the functional investigation of various biosynthetic genes by targeted inactivation. In addition, BLAST analysis of SBCb004 genome data was used to identify multiple candidate monooxygenases, whose involvement in tubulysin assembly was evaluated using a combination of knockout mutagenesis and heterologous expression." @default.
- W2004809474 created "2016-06-24" @default.
- W2004809474 creator A5006923891 @default.
- W2004809474 creator A5009171844 @default.
- W2004809474 creator A5047232406 @default.
- W2004809474 creator A5067406705 @default.
- W2004809474 creator A5077818118 @default.
- W2004809474 creator A5080964927 @default.
- W2004809474 date "2012-03-01" @default.
- W2004809474 modified "2023-10-09" @default.
- W2004809474 title "Heterologous Expression and Genetic Engineering of the Tubulysin Biosynthetic Gene Cluster Using Red/ET Recombineering and Inactivation Mutagenesis" @default.
- W2004809474 cites W1555471169 @default.
- W2004809474 cites W1580639168 @default.
- W2004809474 cites W1963944992 @default.
- W2004809474 cites W1965931924 @default.
- W2004809474 cites W1966262313 @default.
- W2004809474 cites W1974191073 @default.
- W2004809474 cites W1978514556 @default.
- W2004809474 cites W1982784580 @default.
- W2004809474 cites W1985072203 @default.
- W2004809474 cites W1999799832 @default.
- W2004809474 cites W2003514067 @default.
- W2004809474 cites W2010371815 @default.
- W2004809474 cites W2014637330 @default.
- W2004809474 cites W2015670768 @default.
- W2004809474 cites W2020692272 @default.
- W2004809474 cites W2036590099 @default.
- W2004809474 cites W2036984750 @default.
- W2004809474 cites W2046206232 @default.
- W2004809474 cites W2054805269 @default.
- W2004809474 cites W2058704252 @default.
- W2004809474 cites W2067474031 @default.
- W2004809474 cites W2072733444 @default.
- W2004809474 cites W2081228145 @default.
- W2004809474 cites W2085199146 @default.
- W2004809474 cites W2095639245 @default.
- W2004809474 cites W2110078458 @default.
- W2004809474 cites W2111198111 @default.
- W2004809474 cites W2114230622 @default.
- W2004809474 cites W2123778984 @default.
- W2004809474 cites W2128114769 @default.
- W2004809474 cites W2156721582 @default.
- W2004809474 cites W2158714788 @default.
- W2004809474 cites W2165497812 @default.
- W2004809474 cites W2166152206 @default.
- W2004809474 cites W2176108152 @default.
- W2004809474 cites W81291701 @default.
- W2004809474 doi "https://doi.org/10.1016/j.chembiol.2012.01.007" @default.
- W2004809474 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22444591" @default.
- W2004809474 hasPublicationYear "2012" @default.
- W2004809474 type Work @default.
- W2004809474 sameAs 2004809474 @default.
- W2004809474 citedByCount "58" @default.
- W2004809474 countsByYear W20048094742012 @default.
- W2004809474 countsByYear W20048094742013 @default.
- W2004809474 countsByYear W20048094742014 @default.
- W2004809474 countsByYear W20048094742015 @default.
- W2004809474 countsByYear W20048094742016 @default.
- W2004809474 countsByYear W20048094742017 @default.
- W2004809474 countsByYear W20048094742018 @default.
- W2004809474 countsByYear W20048094742019 @default.
- W2004809474 countsByYear W20048094742020 @default.
- W2004809474 countsByYear W20048094742021 @default.
- W2004809474 countsByYear W20048094742022 @default.
- W2004809474 crossrefType "journal-article" @default.
- W2004809474 hasAuthorship W2004809474A5006923891 @default.
- W2004809474 hasAuthorship W2004809474A5009171844 @default.
- W2004809474 hasAuthorship W2004809474A5047232406 @default.
- W2004809474 hasAuthorship W2004809474A5067406705 @default.
- W2004809474 hasAuthorship W2004809474A5077818118 @default.
- W2004809474 hasAuthorship W2004809474A5080964927 @default.
- W2004809474 hasBestOaLocation W20048094741 @default.
- W2004809474 hasConcept C104317684 @default.
- W2004809474 hasConcept C108305142 @default.
- W2004809474 hasConcept C141231307 @default.
- W2004809474 hasConcept C143065580 @default.
- W2004809474 hasConcept C16318435 @default.
- W2004809474 hasConcept C2777120639 @default.
- W2004809474 hasConcept C2777905271 @default.
- W2004809474 hasConcept C2780115692 @default.
- W2004809474 hasConcept C36857842 @default.
- W2004809474 hasConcept C40767141 @default.
- W2004809474 hasConcept C501734568 @default.
- W2004809474 hasConcept C523546767 @default.
- W2004809474 hasConcept C54355233 @default.
- W2004809474 hasConcept C55493867 @default.
- W2004809474 hasConcept C70239388 @default.
- W2004809474 hasConcept C70721500 @default.
- W2004809474 hasConcept C86803240 @default.
- W2004809474 hasConceptScore W2004809474C104317684 @default.
- W2004809474 hasConceptScore W2004809474C108305142 @default.
- W2004809474 hasConceptScore W2004809474C141231307 @default.
- W2004809474 hasConceptScore W2004809474C143065580 @default.
- W2004809474 hasConceptScore W2004809474C16318435 @default.
- W2004809474 hasConceptScore W2004809474C2777120639 @default.
- W2004809474 hasConceptScore W2004809474C2777905271 @default.
- W2004809474 hasConceptScore W2004809474C2780115692 @default.
- W2004809474 hasConceptScore W2004809474C36857842 @default.