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- W2004927691 abstract "Abstract The sensitive and specific direct analysis of urinary normetanephrine and metanephrine (both free and conjugated) was achieved utilizing reversed-phase high performance liquid chromatography and UV detection. The urine specimens were hydrolysed in a boiling water bath and treated with isoamyl alcohol:toluene to eliminate interference from urinary pigments or related chemical compounds. The methoxyamines were isolated by the passage through a small ion-exchange column and then pre-concentrated by a rapid set of solvent (ethyl acetate:acetone) extractions before being injected into the chromatographic column. Detection was achieved with UV absorbance detector at 280 nm and metanephrines were quantified by standard addition method. For both metanephrine and normetanephrine the standard curve was linear over the range of 15-300 (μmol/L and practical lower detection limits were about 100 nmol/L for actual urine samples, which are not too different from those using electrochemical deLector. Samples can be injected at 10-min intervals. The values for a patient with pheochromocytoma and for other patients are given." @default.
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- W2004927691 date "1992-09-01" @default.
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- W2004927691 title "The Determination of Urinary Metanephrine and Normetanephrine by High Performance Liquid Chromatography with Ultraviolet Absorbance Detection" @default.
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- W2004927691 doi "https://doi.org/10.1080/10826079208017202" @default.
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