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• W2004952617 abstract "Sublines of hyperdiploid Ehrlich ascites tumor cells (ED-0.15 cells) growing in Eagle basal medium (total salt concentration: 0.15 M) supplemented with 10 % fetal calf serum were adapted, by stepwise addition of NaCl to the medium, to growth in hypertonic media at salt concentrations of 0.25, 0.30, 0.35, 0.40, 0.45, and 0.50 M. Progressive increases in the NaCl concentration resulted in a progressive increase in the population doubling time of these high-salt-tolerant cultures. Cells growing in isotonic control medium were predominantly ‘epithelial-like’ in contrast to the flat, extended, ‘fibroblast-like’ morphology of cells growing in hypertonic media. These alterations in cell morphology and growth pattern are interpreted as ‘adaptive’ changes in response to ionic changes in the substrate, since ‘re-adaptation’ to growth in isotonic media resulted in a reversion to the morphology and population doubling time characteristic of the original parent cells. It is suggested that such high-salt-tolerant cells may be useful as model systems for study of the effects of substrate alteration on cellular metabolism." @default.
• W2004952617 created "2016-06-24" @default.
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• W2004952617 date "1972-02-01" @default.
• W2004952617 modified "2023-09-25" @default.
• W2004952617 title "Serial cultivation of ehrlich ascites tumor cells in hypertonic media" @default.
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• W2004952617 doi "https://doi.org/10.1016/0014-4827(72)90142-5" @default.
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