Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004954151> ?p ?o ?g. }
- W2004954151 endingPage "608" @default.
- W2004954151 startingPage "598" @default.
- W2004954151 abstract "•Gene expression and metabolites levels relative to cell size are analyzed in liver•Mitochondrial gene expression is repressed cell-autonomously in larger cells•Cell size can be modulated by targeting mitochondria functions and lipid synthesis•Lipids are negative regulators of cell size because they promote cell proliferation BackgroundRegulation of cell size requires coordination of growth and proliferation. Conditional loss of cyclin-dependent kinase 1 in mice permits hepatocyte growth without cell division, allowing us to study cell size in vivo using transcriptomics and metabolomics.ResultsLarger cells displayed increased expression of cytoskeletal genes but unexpectedly repressed expression of many genes involved in mitochondrial functions. This effect appears to be cell autonomous because cultured Drosophila cells induced to increase cell size displayed a similar gene-expression pattern. Larger hepatocytes also displayed a reduction in the expression of lipogenic transcription factors, especially sterol-regulatory element binding proteins. Inhibition of mitochondrial functions and lipid biosynthesis, which is dependent on mitochondrial metabolism, increased the cell size with reciprocal effects on cell proliferation in several cell lines.ConclusionsWe uncover that large cell-size increase is accompanied by downregulation of mitochondrial gene expression, similar to that observed in diabetic individuals. Mitochondrial metabolism and lipid synthesis are used to couple cell size and cell proliferation. This regulatory mechanism may provide a possible mechanism for sensing metazoan cell size. Regulation of cell size requires coordination of growth and proliferation. Conditional loss of cyclin-dependent kinase 1 in mice permits hepatocyte growth without cell division, allowing us to study cell size in vivo using transcriptomics and metabolomics. Larger cells displayed increased expression of cytoskeletal genes but unexpectedly repressed expression of many genes involved in mitochondrial functions. This effect appears to be cell autonomous because cultured Drosophila cells induced to increase cell size displayed a similar gene-expression pattern. Larger hepatocytes also displayed a reduction in the expression of lipogenic transcription factors, especially sterol-regulatory element binding proteins. Inhibition of mitochondrial functions and lipid biosynthesis, which is dependent on mitochondrial metabolism, increased the cell size with reciprocal effects on cell proliferation in several cell lines. We uncover that large cell-size increase is accompanied by downregulation of mitochondrial gene expression, similar to that observed in diabetic individuals. Mitochondrial metabolism and lipid synthesis are used to couple cell size and cell proliferation. This regulatory mechanism may provide a possible mechanism for sensing metazoan cell size." @default.
- W2004954151 created "2016-06-24" @default.
- W2004954151 creator A5003767811 @default.
- W2004954151 creator A5014164691 @default.
- W2004954151 creator A5020497760 @default.
- W2004954151 creator A5055174616 @default.
- W2004954151 creator A5062863261 @default.
- W2004954151 creator A5069897350 @default.
- W2004954151 creator A5083382890 @default.
- W2004954151 creator A5091208710 @default.
- W2004954151 date "2014-03-01" @default.
- W2004954151 modified "2023-10-17" @default.
- W2004954151 title "Identification of Transcriptional and Metabolic Programs Related to Mammalian Cell Size" @default.
- W2004954151 cites W1970821956 @default.
- W2004954151 cites W1971060360 @default.
- W2004954151 cites W1972126834 @default.
- W2004954151 cites W1975631246 @default.
- W2004954151 cites W1977622464 @default.
- W2004954151 cites W1985235104 @default.
- W2004954151 cites W1987522719 @default.
- W2004954151 cites W1999789975 @default.
- W2004954151 cites W2006690685 @default.
- W2004954151 cites W2006873092 @default.
- W2004954151 cites W2024391964 @default.
- W2004954151 cites W2026265704 @default.
- W2004954151 cites W2026578374 @default.
- W2004954151 cites W2034773856 @default.
- W2004954151 cites W2035091349 @default.
- W2004954151 cites W2037938144 @default.
- W2004954151 cites W2048439293 @default.
- W2004954151 cites W2049481764 @default.
- W2004954151 cites W2056040807 @default.
- W2004954151 cites W2062057385 @default.
- W2004954151 cites W2066704041 @default.
- W2004954151 cites W2079019214 @default.
- W2004954151 cites W2085568550 @default.
- W2004954151 cites W2088127501 @default.
- W2004954151 cites W2093629098 @default.
- W2004954151 cites W2110997472 @default.
- W2004954151 cites W2123106337 @default.
- W2004954151 cites W2125076437 @default.
- W2004954151 cites W2125408763 @default.
- W2004954151 cites W2129736201 @default.
- W2004954151 cites W2130844401 @default.
- W2004954151 cites W2131415852 @default.
- W2004954151 cites W2146791037 @default.
- W2004954151 cites W2162791825 @default.
- W2004954151 cites W2172213445 @default.
- W2004954151 doi "https://doi.org/10.1016/j.cub.2014.01.071" @default.
- W2004954151 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3991852" @default.
- W2004954151 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24613310" @default.
- W2004954151 hasPublicationYear "2014" @default.
- W2004954151 type Work @default.
- W2004954151 sameAs 2004954151 @default.
- W2004954151 citedByCount "107" @default.
- W2004954151 countsByYear W20049541512014 @default.
- W2004954151 countsByYear W20049541512015 @default.
- W2004954151 countsByYear W20049541512016 @default.
- W2004954151 countsByYear W20049541512017 @default.
- W2004954151 countsByYear W20049541512018 @default.
- W2004954151 countsByYear W20049541512019 @default.
- W2004954151 countsByYear W20049541512020 @default.
- W2004954151 countsByYear W20049541512021 @default.
- W2004954151 countsByYear W20049541512022 @default.
- W2004954151 countsByYear W20049541512023 @default.
- W2004954151 crossrefType "journal-article" @default.
- W2004954151 hasAuthorship W2004954151A5003767811 @default.
- W2004954151 hasAuthorship W2004954151A5014164691 @default.
- W2004954151 hasAuthorship W2004954151A5020497760 @default.
- W2004954151 hasAuthorship W2004954151A5055174616 @default.
- W2004954151 hasAuthorship W2004954151A5062863261 @default.
- W2004954151 hasAuthorship W2004954151A5069897350 @default.
- W2004954151 hasAuthorship W2004954151A5083382890 @default.
- W2004954151 hasAuthorship W2004954151A5091208710 @default.
- W2004954151 hasBestOaLocation W20049541511 @default.
- W2004954151 hasConcept C104317684 @default.
- W2004954151 hasConcept C1491633281 @default.
- W2004954151 hasConcept C150194340 @default.
- W2004954151 hasConcept C162317418 @default.
- W2004954151 hasConcept C202751555 @default.
- W2004954151 hasConcept C2776200302 @default.
- W2004954151 hasConcept C28859421 @default.
- W2004954151 hasConcept C4733338 @default.
- W2004954151 hasConcept C54355233 @default.
- W2004954151 hasConcept C55493867 @default.
- W2004954151 hasConcept C62112901 @default.
- W2004954151 hasConcept C85813293 @default.
- W2004954151 hasConcept C86803240 @default.
- W2004954151 hasConcept C95444343 @default.
- W2004954151 hasConceptScore W2004954151C104317684 @default.
- W2004954151 hasConceptScore W2004954151C1491633281 @default.
- W2004954151 hasConceptScore W2004954151C150194340 @default.
- W2004954151 hasConceptScore W2004954151C162317418 @default.
- W2004954151 hasConceptScore W2004954151C202751555 @default.
- W2004954151 hasConceptScore W2004954151C2776200302 @default.
- W2004954151 hasConceptScore W2004954151C28859421 @default.
- W2004954151 hasConceptScore W2004954151C4733338 @default.
- W2004954151 hasConceptScore W2004954151C54355233 @default.